Cluster size distribution of cells disseminating from a primary tumor.

The first stage of the metastatic cascade often involves motile cells emerging from a primary tumor either as single cells or as clusters. These cells enter the circulation, transit to other parts of the body and finally are responsible for growth of secondary tumors in distant organs. The mode of d...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mrinmoy Mukherjee, Herbert Levine
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/eca0ef45b8b143309ae61e93059c9399
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:eca0ef45b8b143309ae61e93059c9399
record_format	dspace
spelling	oai:doaj.org-article:eca0ef45b8b143309ae61e93059c93992021-12-02T19:57:57ZCluster size distribution of cells disseminating from a primary tumor.1553-734X1553-735810.1371/journal.pcbi.1009011https://doaj.org/article/eca0ef45b8b143309ae61e93059c93992021-11-01T00:00:00Zhttps://doi.org/10.1371/journal.pcbi.1009011https://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358The first stage of the metastatic cascade often involves motile cells emerging from a primary tumor either as single cells or as clusters. These cells enter the circulation, transit to other parts of the body and finally are responsible for growth of secondary tumors in distant organs. The mode of dissemination is believed to depend on the EMT nature (epithelial, hybrid or mesenchymal) of the cells. Here, we calculate the cluster size distribution of these migrating cells, using a mechanistic computational model, in presence of different degree of EMT-ness of the cells; EMT is treated as given rise to changes in their active motile forces (μ) and cell-medium surface tension (Γ). We find that, for (μ > μmin, Γ > 1), when the cells are hybrid in nature, the mean cluster size, [Formula: see text], where μmin increases with increase in Γ. For Γ ≤ 0, [Formula: see text], the cells behave as completely mesenchymal. In presence of spectrum of hybrid states with different degree of EMT-ness (motility) in primary tumor, the cells which are relatively more mesenchymal (higher μ) in nature, form larger clusters, whereas the smaller clusters are relatively more epithelial (lower μ). Moreover, the heterogeneity in μ is comparatively higher for smaller clusters with respect to that for larger clusters. We also observe that more extended cell shapes promote the formation of smaller clusters. Overall, this study establishes a framework which connects the nature and size of migrating clusters disseminating from a primary tumor with the phenotypic composition of the tumor, and can lead to the better understanding of metastasis.Mrinmoy MukherjeeHerbert LevinePublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 17, Iss 11, p e1009011 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Mrinmoy Mukherjee Herbert Levine Cluster size distribution of cells disseminating from a primary tumor.
description	The first stage of the metastatic cascade often involves motile cells emerging from a primary tumor either as single cells or as clusters. These cells enter the circulation, transit to other parts of the body and finally are responsible for growth of secondary tumors in distant organs. The mode of dissemination is believed to depend on the EMT nature (epithelial, hybrid or mesenchymal) of the cells. Here, we calculate the cluster size distribution of these migrating cells, using a mechanistic computational model, in presence of different degree of EMT-ness of the cells; EMT is treated as given rise to changes in their active motile forces (μ) and cell-medium surface tension (Γ). We find that, for (μ > μmin, Γ > 1), when the cells are hybrid in nature, the mean cluster size, [Formula: see text], where μmin increases with increase in Γ. For Γ ≤ 0, [Formula: see text], the cells behave as completely mesenchymal. In presence of spectrum of hybrid states with different degree of EMT-ness (motility) in primary tumor, the cells which are relatively more mesenchymal (higher μ) in nature, form larger clusters, whereas the smaller clusters are relatively more epithelial (lower μ). Moreover, the heterogeneity in μ is comparatively higher for smaller clusters with respect to that for larger clusters. We also observe that more extended cell shapes promote the formation of smaller clusters. Overall, this study establishes a framework which connects the nature and size of migrating clusters disseminating from a primary tumor with the phenotypic composition of the tumor, and can lead to the better understanding of metastasis.
format	article
author	Mrinmoy Mukherjee Herbert Levine
author_facet	Mrinmoy Mukherjee Herbert Levine
author_sort	Mrinmoy Mukherjee
title	Cluster size distribution of cells disseminating from a primary tumor.
title_short	Cluster size distribution of cells disseminating from a primary tumor.
title_full	Cluster size distribution of cells disseminating from a primary tumor.
title_fullStr	Cluster size distribution of cells disseminating from a primary tumor.
title_full_unstemmed	Cluster size distribution of cells disseminating from a primary tumor.
title_sort	cluster size distribution of cells disseminating from a primary tumor.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/eca0ef45b8b143309ae61e93059c9399
work_keys_str_mv	AT mrinmoymukherjee clustersizedistributionofcellsdisseminatingfromaprimarytumor AT herbertlevine clustersizedistributionofcellsdisseminatingfromaprimarytumor
_version_	1718375813933432832

Cluster size distribution of cells disseminating from a primary tumor.

Ejemplares similares