Evolutionary Dynamics of <named-content content-type="genus-species">Vibrio cholerae</named-content> O1 following a Single-Source Introduction to Haiti

ABSTRACT Prior to the epidemic that emerged in Haiti in October of 2010, cholera had not been documented in this country. After its introduction, a strain of Vibrio cholerae O1 spread rapidly throughout Haiti, where it caused over 600,000 cases of disease and >7,500 deaths in the first two years...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Lee S. Katz, Aaron Petkau, John Beaulaurier, Shaun Tyler, Elena S. Antonova, Maryann A. Turnsek, Yan Guo, Susana Wang, Ellen E. Paxinos, Fabini Orata, Lori M. Gladney, Steven Stroika, Jason P. Folster, Lori Rowe, Molly M. Freeman, Natalie Knox, Mike Frace, Jacques Boncy, Morag Graham, Brian K. Hammer, Yan Boucher, Ali Bashir, William P. Hanage, Gary Van Domselaar, Cheryl L. Tarr
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2013
Materias:
Acceso en línea:https://doaj.org/article/eca35f0f8b1a42409063d3f3f98b9e45
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:eca35f0f8b1a42409063d3f3f98b9e45
record_format dspace
spelling oai:doaj.org-article:eca35f0f8b1a42409063d3f3f98b9e452021-11-15T15:43:08ZEvolutionary Dynamics of <named-content content-type="genus-species">Vibrio cholerae</named-content> O1 following a Single-Source Introduction to Haiti10.1128/mBio.00398-132150-7511https://doaj.org/article/eca35f0f8b1a42409063d3f3f98b9e452013-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00398-13https://doaj.org/toc/2150-7511ABSTRACT Prior to the epidemic that emerged in Haiti in October of 2010, cholera had not been documented in this country. After its introduction, a strain of Vibrio cholerae O1 spread rapidly throughout Haiti, where it caused over 600,000 cases of disease and >7,500 deaths in the first two years of the epidemic. We applied whole-genome sequencing to a temporal series of V. cholerae isolates from Haiti to gain insight into the mode and tempo of evolution in this isolated population of V. cholerae O1. Phylogenetic and Bayesian analyses supported the hypothesis that all isolates in the sample set diverged from a common ancestor within a time frame that is consistent with epidemiological observations. A pangenome analysis showed nearly homogeneous genomic content, with no evidence of gene acquisition among Haiti isolates. Nine nearly closed genomes assembled from continuous-long-read data showed evidence of genome rearrangements and supported the observation of no gene acquisition among isolates. Thus, intrinsic mutational processes can account for virtually all of the observed genetic polymorphism, with no demonstrable contribution from horizontal gene transfer (HGT). Consistent with this, the 12 Haiti isolates tested by laboratory HGT assays were severely impaired for transformation, although unlike previously characterized noncompetent V. cholerae isolates, each expressed hapR and possessed a functional quorum-sensing system. Continued monitoring of V. cholerae in Haiti will illuminate the processes influencing the origin and fate of genome variants, which will facilitate interpretation of genetic variation in future epidemics. IMPORTANCE Vibrio cholerae is the cause of substantial morbidity and mortality worldwide, with over three million cases of disease each year. An understanding of the mode and rate of evolutionary change is critical for proper interpretation of genome sequence data and attribution of outbreak sources. The Haiti epidemic provides an unprecedented opportunity to study an isolated, single-source outbreak of Vibrio cholerae O1 over an established time frame. By using multiple approaches to assay genetic variation, we found no evidence that the Haiti strain has acquired any genes by horizontal gene transfer, an observation that led us to discover that it is also poorly transformable. We have found no evidence that environmental strains have played a role in the evolution of the outbreak strain.Lee S. KatzAaron PetkauJohn BeaulaurierShaun TylerElena S. AntonovaMaryann A. TurnsekYan GuoSusana WangEllen E. PaxinosFabini OrataLori M. GladneySteven StroikaJason P. FolsterLori RoweMolly M. FreemanNatalie KnoxMike FraceJacques BoncyMorag GrahamBrian K. HammerYan BoucherAli BashirWilliam P. HanageGary Van DomselaarCheryl L. TarrAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 4 (2013)
institution DOAJ
collection DOAJ
language EN
topic Microbiology
QR1-502
spellingShingle Microbiology
QR1-502
Lee S. Katz
Aaron Petkau
John Beaulaurier
Shaun Tyler
Elena S. Antonova
Maryann A. Turnsek
Yan Guo
Susana Wang
Ellen E. Paxinos
Fabini Orata
Lori M. Gladney
Steven Stroika
Jason P. Folster
Lori Rowe
Molly M. Freeman
Natalie Knox
Mike Frace
Jacques Boncy
Morag Graham
Brian K. Hammer
Yan Boucher
Ali Bashir
William P. Hanage
Gary Van Domselaar
Cheryl L. Tarr
Evolutionary Dynamics of <named-content content-type="genus-species">Vibrio cholerae</named-content> O1 following a Single-Source Introduction to Haiti
description ABSTRACT Prior to the epidemic that emerged in Haiti in October of 2010, cholera had not been documented in this country. After its introduction, a strain of Vibrio cholerae O1 spread rapidly throughout Haiti, where it caused over 600,000 cases of disease and >7,500 deaths in the first two years of the epidemic. We applied whole-genome sequencing to a temporal series of V. cholerae isolates from Haiti to gain insight into the mode and tempo of evolution in this isolated population of V. cholerae O1. Phylogenetic and Bayesian analyses supported the hypothesis that all isolates in the sample set diverged from a common ancestor within a time frame that is consistent with epidemiological observations. A pangenome analysis showed nearly homogeneous genomic content, with no evidence of gene acquisition among Haiti isolates. Nine nearly closed genomes assembled from continuous-long-read data showed evidence of genome rearrangements and supported the observation of no gene acquisition among isolates. Thus, intrinsic mutational processes can account for virtually all of the observed genetic polymorphism, with no demonstrable contribution from horizontal gene transfer (HGT). Consistent with this, the 12 Haiti isolates tested by laboratory HGT assays were severely impaired for transformation, although unlike previously characterized noncompetent V. cholerae isolates, each expressed hapR and possessed a functional quorum-sensing system. Continued monitoring of V. cholerae in Haiti will illuminate the processes influencing the origin and fate of genome variants, which will facilitate interpretation of genetic variation in future epidemics. IMPORTANCE Vibrio cholerae is the cause of substantial morbidity and mortality worldwide, with over three million cases of disease each year. An understanding of the mode and rate of evolutionary change is critical for proper interpretation of genome sequence data and attribution of outbreak sources. The Haiti epidemic provides an unprecedented opportunity to study an isolated, single-source outbreak of Vibrio cholerae O1 over an established time frame. By using multiple approaches to assay genetic variation, we found no evidence that the Haiti strain has acquired any genes by horizontal gene transfer, an observation that led us to discover that it is also poorly transformable. We have found no evidence that environmental strains have played a role in the evolution of the outbreak strain.
format article
author Lee S. Katz
Aaron Petkau
John Beaulaurier
Shaun Tyler
Elena S. Antonova
Maryann A. Turnsek
Yan Guo
Susana Wang
Ellen E. Paxinos
Fabini Orata
Lori M. Gladney
Steven Stroika
Jason P. Folster
Lori Rowe
Molly M. Freeman
Natalie Knox
Mike Frace
Jacques Boncy
Morag Graham
Brian K. Hammer
Yan Boucher
Ali Bashir
William P. Hanage
Gary Van Domselaar
Cheryl L. Tarr
author_facet Lee S. Katz
Aaron Petkau
John Beaulaurier
Shaun Tyler
Elena S. Antonova
Maryann A. Turnsek
Yan Guo
Susana Wang
Ellen E. Paxinos
Fabini Orata
Lori M. Gladney
Steven Stroika
Jason P. Folster
Lori Rowe
Molly M. Freeman
Natalie Knox
Mike Frace
Jacques Boncy
Morag Graham
Brian K. Hammer
Yan Boucher
Ali Bashir
William P. Hanage
Gary Van Domselaar
Cheryl L. Tarr
author_sort Lee S. Katz
title Evolutionary Dynamics of <named-content content-type="genus-species">Vibrio cholerae</named-content> O1 following a Single-Source Introduction to Haiti
title_short Evolutionary Dynamics of <named-content content-type="genus-species">Vibrio cholerae</named-content> O1 following a Single-Source Introduction to Haiti
title_full Evolutionary Dynamics of <named-content content-type="genus-species">Vibrio cholerae</named-content> O1 following a Single-Source Introduction to Haiti
title_fullStr Evolutionary Dynamics of <named-content content-type="genus-species">Vibrio cholerae</named-content> O1 following a Single-Source Introduction to Haiti
title_full_unstemmed Evolutionary Dynamics of <named-content content-type="genus-species">Vibrio cholerae</named-content> O1 following a Single-Source Introduction to Haiti
title_sort evolutionary dynamics of <named-content content-type="genus-species">vibrio cholerae</named-content> o1 following a single-source introduction to haiti
publisher American Society for Microbiology
publishDate 2013
url https://doaj.org/article/eca35f0f8b1a42409063d3f3f98b9e45
work_keys_str_mv AT leeskatz evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT aaronpetkau evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT johnbeaulaurier evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT shauntyler evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT elenasantonova evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT maryannaturnsek evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT yanguo evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT susanawang evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT ellenepaxinos evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT fabiniorata evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT lorimgladney evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT stevenstroika evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT jasonpfolster evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT lorirowe evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT mollymfreeman evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT natalieknox evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT mikefrace evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT jacquesboncy evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT moraggraham evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT briankhammer evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT yanboucher evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT alibashir evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT williamphanage evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT garyvandomselaar evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
AT cherylltarr evolutionarydynamicsofnamedcontentcontenttypegenusspeciesvibriocholeraenamedcontento1followingasinglesourceintroductiontohaiti
_version_ 1718427618530820096