Freezing of cell sheets using a 3D freezer produces high cell viability after thawing

In cell therapy, transplanting an appropriate number of cells to the target site is crucial. One way to achieve this is to transplant cell sheets. Transplantation of cell sheets has already been utilized for various diseases in clinical practice. However, reducing the cost of cell sheet utilization...

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Autores principales:	Koji Ueno, Soichi Ike, Naohiro Yamamoto, Yutaro Matsuno, Hiroshi Kurazumi, Ryo Suzuki, Shunsaku Katsura, Bungo Shirasawa, Kimikazu Hamano
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell sheet Freezing 3D freezer Survival Regenerative medicine Temperature Biology (General) QH301-705.5 Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/eca82acbfb9042ee80bc703f33e41577
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spelling	oai:doaj.org-article:eca82acbfb9042ee80bc703f33e415772021-11-10T04:29:25ZFreezing of cell sheets using a 3D freezer produces high cell viability after thawing2405-580810.1016/j.bbrep.2021.101169https://doaj.org/article/eca82acbfb9042ee80bc703f33e415772021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405580821002636https://doaj.org/toc/2405-5808In cell therapy, transplanting an appropriate number of cells to the target site is crucial. One way to achieve this is to transplant cell sheets. Transplantation of cell sheets has already been utilized for various diseases in clinical practice. However, reducing the cost of cell sheet utilization is essential so as to facilitate the spread of regenerative medicine. Several ways to reduce costs are available, one of which is the use of allogenic cells. Another alternative is the use of cell sheets, which necessitates the development of methods for freezing cell sheets. This is the first study to report the use of a 3D Freezer for freezing cells. 3D Freezers have been used in the field of food processing and technology for a long time. The 3D Freezer freezes objects using cold air at a uniform temperature from all directions. In this study, we analyzed the cooling speed of human fibroblast sheets in 11 cell preservation solutions using a 3D Freezer and a Program Freezer. The cooling speed was −2 °C per min in the 3D Freezer. Supercooling in 10 cell preservation solutions was lower in the 3D Freezer than in the Program Freezer. Cell viability after freeze–thaw of the cell sheets using 3D Freezer was more than 70% in five cell preservation solutions. The levels of hepatocyte growth factor and transforming growth factor-β1 were the same not only in the fibroblast sheets frozen using the five cell preservation solutions but also in the non-frozen fibroblast sheets. These results suggest that the 3D Freezer can freeze implantable cell sheets immediately after thawing.Koji UenoSoichi IkeNaohiro YamamotoYutaro MatsunoHiroshi KurazumiRyo SuzukiShunsaku KatsuraBungo ShirasawaKimikazu HamanoElsevierarticleCell sheetFreezing3D freezerSurvivalRegenerative medicineTemperatureBiology (General)QH301-705.5BiochemistryQD415-436ENBiochemistry and Biophysics Reports, Vol 28, Iss , Pp 101169- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell sheet Freezing 3D freezer Survival Regenerative medicine Temperature Biology (General) QH301-705.5 Biochemistry QD415-436
spellingShingle	Cell sheet Freezing 3D freezer Survival Regenerative medicine Temperature Biology (General) QH301-705.5 Biochemistry QD415-436 Koji Ueno Soichi Ike Naohiro Yamamoto Yutaro Matsuno Hiroshi Kurazumi Ryo Suzuki Shunsaku Katsura Bungo Shirasawa Kimikazu Hamano Freezing of cell sheets using a 3D freezer produces high cell viability after thawing
description	In cell therapy, transplanting an appropriate number of cells to the target site is crucial. One way to achieve this is to transplant cell sheets. Transplantation of cell sheets has already been utilized for various diseases in clinical practice. However, reducing the cost of cell sheet utilization is essential so as to facilitate the spread of regenerative medicine. Several ways to reduce costs are available, one of which is the use of allogenic cells. Another alternative is the use of cell sheets, which necessitates the development of methods for freezing cell sheets. This is the first study to report the use of a 3D Freezer for freezing cells. 3D Freezers have been used in the field of food processing and technology for a long time. The 3D Freezer freezes objects using cold air at a uniform temperature from all directions. In this study, we analyzed the cooling speed of human fibroblast sheets in 11 cell preservation solutions using a 3D Freezer and a Program Freezer. The cooling speed was −2 °C per min in the 3D Freezer. Supercooling in 10 cell preservation solutions was lower in the 3D Freezer than in the Program Freezer. Cell viability after freeze–thaw of the cell sheets using 3D Freezer was more than 70% in five cell preservation solutions. The levels of hepatocyte growth factor and transforming growth factor-β1 were the same not only in the fibroblast sheets frozen using the five cell preservation solutions but also in the non-frozen fibroblast sheets. These results suggest that the 3D Freezer can freeze implantable cell sheets immediately after thawing.
format	article
author	Koji Ueno Soichi Ike Naohiro Yamamoto Yutaro Matsuno Hiroshi Kurazumi Ryo Suzuki Shunsaku Katsura Bungo Shirasawa Kimikazu Hamano
author_facet	Koji Ueno Soichi Ike Naohiro Yamamoto Yutaro Matsuno Hiroshi Kurazumi Ryo Suzuki Shunsaku Katsura Bungo Shirasawa Kimikazu Hamano
author_sort	Koji Ueno
title	Freezing of cell sheets using a 3D freezer produces high cell viability after thawing
title_short	Freezing of cell sheets using a 3D freezer produces high cell viability after thawing
title_full	Freezing of cell sheets using a 3D freezer produces high cell viability after thawing
title_fullStr	Freezing of cell sheets using a 3D freezer produces high cell viability after thawing
title_full_unstemmed	Freezing of cell sheets using a 3D freezer produces high cell viability after thawing
title_sort	freezing of cell sheets using a 3d freezer produces high cell viability after thawing
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/eca82acbfb9042ee80bc703f33e41577
work_keys_str_mv	AT kojiueno freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT soichiike freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT naohiroyamamoto freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT yutaromatsuno freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT hiroshikurazumi freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT ryosuzuki freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT shunsakukatsura freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT bungoshirasawa freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing AT kimikazuhamano freezingofcellsheetsusinga3dfreezerproduceshighcellviabilityafterthawing
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Freezing of cell sheets using a 3D freezer produces high cell viability after thawing

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