A novel plant E3 ligase stabilizes Escherichia coli heat shock factor σ32

Abstract The heat shock response is crucial for organisms against heat-damaged proteins and maintaining homeostasis at a high temperature. Heterologous expression of eukaryotic molecular chaperones protects Escherichia coli from heat stress. Here we report that expression of the plant E3 ligase BnTR...

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Autores principales: Yulong Niu, Xibing Xu, Chengcheng Liu, Tao Wang, Ke Liang, Jianmei Wang, Zhibin Liu, Xufeng Li, Yi Yang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/ecc2883457aa4e63b817f314909fe81f
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spelling oai:doaj.org-article:ecc2883457aa4e63b817f314909fe81f2021-12-02T12:32:27ZA novel plant E3 ligase stabilizes Escherichia coli heat shock factor σ3210.1038/s41598-017-03056-62045-2322https://doaj.org/article/ecc2883457aa4e63b817f314909fe81f2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03056-6https://doaj.org/toc/2045-2322Abstract The heat shock response is crucial for organisms against heat-damaged proteins and maintaining homeostasis at a high temperature. Heterologous expression of eukaryotic molecular chaperones protects Escherichia coli from heat stress. Here we report that expression of the plant E3 ligase BnTR1 significantly increases the thermotolerance of E. coli. Different from eukaryotic chaperones, BnTR1 expression induces the accumulation of heat shock factor σ32 and heat shock proteins. The active site of BnTR1 in E. coli is the zinc fingers of the RING domain, which interacts with DnaK resulting in stabilizing σ32. Our findings indicate the expression of BnTR1 confers thermoprotective effects on E. coli cells, and it may provide useful clues to engineer thermophilic bacterial strains.Yulong NiuXibing XuChengcheng LiuTao WangKe LiangJianmei WangZhibin LiuXufeng LiYi YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yulong Niu
Xibing Xu
Chengcheng Liu
Tao Wang
Ke Liang
Jianmei Wang
Zhibin Liu
Xufeng Li
Yi Yang
A novel plant E3 ligase stabilizes Escherichia coli heat shock factor σ32
description Abstract The heat shock response is crucial for organisms against heat-damaged proteins and maintaining homeostasis at a high temperature. Heterologous expression of eukaryotic molecular chaperones protects Escherichia coli from heat stress. Here we report that expression of the plant E3 ligase BnTR1 significantly increases the thermotolerance of E. coli. Different from eukaryotic chaperones, BnTR1 expression induces the accumulation of heat shock factor σ32 and heat shock proteins. The active site of BnTR1 in E. coli is the zinc fingers of the RING domain, which interacts with DnaK resulting in stabilizing σ32. Our findings indicate the expression of BnTR1 confers thermoprotective effects on E. coli cells, and it may provide useful clues to engineer thermophilic bacterial strains.
format article
author Yulong Niu
Xibing Xu
Chengcheng Liu
Tao Wang
Ke Liang
Jianmei Wang
Zhibin Liu
Xufeng Li
Yi Yang
author_facet Yulong Niu
Xibing Xu
Chengcheng Liu
Tao Wang
Ke Liang
Jianmei Wang
Zhibin Liu
Xufeng Li
Yi Yang
author_sort Yulong Niu
title A novel plant E3 ligase stabilizes Escherichia coli heat shock factor σ32
title_short A novel plant E3 ligase stabilizes Escherichia coli heat shock factor σ32
title_full A novel plant E3 ligase stabilizes Escherichia coli heat shock factor σ32
title_fullStr A novel plant E3 ligase stabilizes Escherichia coli heat shock factor σ32
title_full_unstemmed A novel plant E3 ligase stabilizes Escherichia coli heat shock factor σ32
title_sort novel plant e3 ligase stabilizes escherichia coli heat shock factor σ32
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/ecc2883457aa4e63b817f314909fe81f
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