Nociceptive DRG neurons express muscle lim protein upon axonal injury

Abstract Muscle lim protein (MLP) has long been regarded as a cytosolic and nuclear muscular protein. Here, we show that MLP is also expressed in a subpopulation of adult rat dorsal root ganglia (DRG) neurons in response to axonal injury, while the protein was not detectable in naïve cells. Detailed...

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Autores principales:	Evgeny Levin, Anastasia Andreadaki, Philipp Gobrecht, Frank Bosse, Dietmar Fischer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ecc4ac228ffb400ab40fff9b4b8d38f9
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spelling	oai:doaj.org-article:ecc4ac228ffb400ab40fff9b4b8d38f92021-12-02T11:53:00ZNociceptive DRG neurons express muscle lim protein upon axonal injury10.1038/s41598-017-00590-12045-2322https://doaj.org/article/ecc4ac228ffb400ab40fff9b4b8d38f92017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00590-1https://doaj.org/toc/2045-2322Abstract Muscle lim protein (MLP) has long been regarded as a cytosolic and nuclear muscular protein. Here, we show that MLP is also expressed in a subpopulation of adult rat dorsal root ganglia (DRG) neurons in response to axonal injury, while the protein was not detectable in naïve cells. Detailed immunohistochemical analysis of L4/L5 DRG revealed ~3% of MLP-positive neurons 2 days after complete sciatic nerve crush and maximum ~10% after 4–14 days. Similarly, in mixed cultures from cervical, thoracic, lumbar and sacral DRG ~6% of neurons were MLP-positive after 2 days and maximal 17% after 3 days. In both, histological sections and cell cultures, the protein was detected in the cytosol and axons of small diameter cells, while the nucleus remained devoid. Moreover, the vast majority could not be assigned to any of the well characterized canonical DRG subpopulations at 7 days after nerve injury. However, further analysis in cell culture revealed that the largest population of MLP expressing cells originated from non-peptidergic IB4-positive nociceptive neurons, which lose their ability to bind the lectin upon axotomy. Thus, MLP is mostly expressed in a subset of axotomized nociceptive neurons and can be used as a novel marker for this population of cells.Evgeny LevinAnastasia AndreadakiPhilipp GobrechtFrank BosseDietmar FischerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Evgeny Levin Anastasia Andreadaki Philipp Gobrecht Frank Bosse Dietmar Fischer Nociceptive DRG neurons express muscle lim protein upon axonal injury
description	Abstract Muscle lim protein (MLP) has long been regarded as a cytosolic and nuclear muscular protein. Here, we show that MLP is also expressed in a subpopulation of adult rat dorsal root ganglia (DRG) neurons in response to axonal injury, while the protein was not detectable in naïve cells. Detailed immunohistochemical analysis of L4/L5 DRG revealed ~3% of MLP-positive neurons 2 days after complete sciatic nerve crush and maximum ~10% after 4–14 days. Similarly, in mixed cultures from cervical, thoracic, lumbar and sacral DRG ~6% of neurons were MLP-positive after 2 days and maximal 17% after 3 days. In both, histological sections and cell cultures, the protein was detected in the cytosol and axons of small diameter cells, while the nucleus remained devoid. Moreover, the vast majority could not be assigned to any of the well characterized canonical DRG subpopulations at 7 days after nerve injury. However, further analysis in cell culture revealed that the largest population of MLP expressing cells originated from non-peptidergic IB4-positive nociceptive neurons, which lose their ability to bind the lectin upon axotomy. Thus, MLP is mostly expressed in a subset of axotomized nociceptive neurons and can be used as a novel marker for this population of cells.
format	article
author	Evgeny Levin Anastasia Andreadaki Philipp Gobrecht Frank Bosse Dietmar Fischer
author_facet	Evgeny Levin Anastasia Andreadaki Philipp Gobrecht Frank Bosse Dietmar Fischer
author_sort	Evgeny Levin
title	Nociceptive DRG neurons express muscle lim protein upon axonal injury
title_short	Nociceptive DRG neurons express muscle lim protein upon axonal injury
title_full	Nociceptive DRG neurons express muscle lim protein upon axonal injury
title_fullStr	Nociceptive DRG neurons express muscle lim protein upon axonal injury
title_full_unstemmed	Nociceptive DRG neurons express muscle lim protein upon axonal injury
title_sort	nociceptive drg neurons express muscle lim protein upon axonal injury
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ecc4ac228ffb400ab40fff9b4b8d38f9
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_version_	1718394959800827904

Nociceptive DRG neurons express muscle lim protein upon axonal injury

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