The effect of sodium carboxymethyl starch with high degree of substitution on defecation.

Sodium carboxymethyl starch (CMS-Na), a kind of food additive with high degree of substitution, is also known as a prebiotic. The aim of this study was to determine the effect of CMS-Na on defecation. Constipated mouse model was prepared by loperamide. Normal rats were also used in the study. Short-...

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Autores principales:	Wu-Dang Lu, Man-Li Wu, Jun-Xia Zhang, Ting-Ting Huang, Shuai-Shuai Du, Yong-Xiao Cao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ecc8658c2a6b4130b22158ac22b09299
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spelling	oai:doaj.org-article:ecc8658c2a6b4130b22158ac22b092992021-12-02T20:14:49ZThe effect of sodium carboxymethyl starch with high degree of substitution on defecation.1932-620310.1371/journal.pone.0257012https://doaj.org/article/ecc8658c2a6b4130b22158ac22b092992021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257012https://doaj.org/toc/1932-6203Sodium carboxymethyl starch (CMS-Na), a kind of food additive with high degree of substitution, is also known as a prebiotic. The aim of this study was to determine the effect of CMS-Na on defecation. Constipated mouse model was prepared by loperamide. Normal rats were also used in the study. Short-chain fatty acids in rat feces were detected by gas chromatography. The bacterial communities in rat feces were identified by 16S rDNA gene sequencing. 5-hydroxytryptamine (5-HT) and tryptophan hydroxylase 1 (Tph1) were measured by ELISA. The results showed that CMS-Na increased the fecal granule counts and intestinal propulsion rate in constipated mice. The contents of water, acetic acid, propionic acid and n-butyrate in feces, Tph1 in colon and 5-HT in serum of rats were increased. In addition, CMS-Na shortened the colonic transport time in rats. The 16S rDNA gene sequencing results indicated that CMS-Na increased the relative abundance of Alloprevotella and decreased the proportion of Lactobacillus. However, the biodiversity of the normal intestinal flora was not altered. In conclusion, CMS-Na can promote defecation in constipated mice. The mechanism may be related to the regulation of Alloprevotella and Lactobacillus in colon, the increase of short-chain fatty acids, and the promotion of the synthesis of Tph1 and 5-HT.Wu-Dang LuMan-Li WuJun-Xia ZhangTing-Ting HuangShuai-Shuai DuYong-Xiao CaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257012 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wu-Dang Lu Man-Li Wu Jun-Xia Zhang Ting-Ting Huang Shuai-Shuai Du Yong-Xiao Cao The effect of sodium carboxymethyl starch with high degree of substitution on defecation.
description	Sodium carboxymethyl starch (CMS-Na), a kind of food additive with high degree of substitution, is also known as a prebiotic. The aim of this study was to determine the effect of CMS-Na on defecation. Constipated mouse model was prepared by loperamide. Normal rats were also used in the study. Short-chain fatty acids in rat feces were detected by gas chromatography. The bacterial communities in rat feces were identified by 16S rDNA gene sequencing. 5-hydroxytryptamine (5-HT) and tryptophan hydroxylase 1 (Tph1) were measured by ELISA. The results showed that CMS-Na increased the fecal granule counts and intestinal propulsion rate in constipated mice. The contents of water, acetic acid, propionic acid and n-butyrate in feces, Tph1 in colon and 5-HT in serum of rats were increased. In addition, CMS-Na shortened the colonic transport time in rats. The 16S rDNA gene sequencing results indicated that CMS-Na increased the relative abundance of Alloprevotella and decreased the proportion of Lactobacillus. However, the biodiversity of the normal intestinal flora was not altered. In conclusion, CMS-Na can promote defecation in constipated mice. The mechanism may be related to the regulation of Alloprevotella and Lactobacillus in colon, the increase of short-chain fatty acids, and the promotion of the synthesis of Tph1 and 5-HT.
format	article
author	Wu-Dang Lu Man-Li Wu Jun-Xia Zhang Ting-Ting Huang Shuai-Shuai Du Yong-Xiao Cao
author_facet	Wu-Dang Lu Man-Li Wu Jun-Xia Zhang Ting-Ting Huang Shuai-Shuai Du Yong-Xiao Cao
author_sort	Wu-Dang Lu
title	The effect of sodium carboxymethyl starch with high degree of substitution on defecation.
title_short	The effect of sodium carboxymethyl starch with high degree of substitution on defecation.
title_full	The effect of sodium carboxymethyl starch with high degree of substitution on defecation.
title_fullStr	The effect of sodium carboxymethyl starch with high degree of substitution on defecation.
title_full_unstemmed	The effect of sodium carboxymethyl starch with high degree of substitution on defecation.
title_sort	effect of sodium carboxymethyl starch with high degree of substitution on defecation.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ecc8658c2a6b4130b22158ac22b09299
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The effect of sodium carboxymethyl starch with high degree of substitution on defecation.

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