Effect of clinical parameters on the ocular surface microbiome in children and adults

Kara M Cavuoto,1,* Roberto Mendez,2,* Darlene Miller,1 Anat Galor,1,3 Santanu Banerjee2 1Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami, Miami, FL, 2Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL, 3Department of Ophthalmology, Miami V...

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Autores principales:	Cavuoto KM, Mendez R, Miller D, Galor A, Banerjee S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Ocular microbiome conjunctival microbiome eyelid margin microbiome skin microbiome 16s rRNA children adult Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/eccefdc53d4642389a4dabfd3a5d3a09
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spelling	oai:doaj.org-article:eccefdc53d4642389a4dabfd3a5d3a092021-12-02T07:26:14ZEffect of clinical parameters on the ocular surface microbiome in children and adults1177-5483https://doaj.org/article/eccefdc53d4642389a4dabfd3a5d3a092018-07-01T00:00:00Zhttps://www.dovepress.com/effect-of-clinical-parameters-on-the-ocular-surface-microbiome-in-chil-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Kara M Cavuoto,1,* Roberto Mendez,2,* Darlene Miller,1 Anat Galor,1,3 Santanu Banerjee2 1Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami, Miami, FL, 2Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL, 3Department of Ophthalmology, Miami Veterans Administration Medical Center, Miami, FL, USA *These authors contributed equally to this work Purpose: To perform a pilot study to characterize the effect of clinical parameters on the ocular surface microbiome (OSM) in children and adults using 16s ribosomal RNA sequencing.Methods: Prospective, cross-sectional study using 16s sequencing to evaluate the OSM. Comparisons were made in bacterial composition by 1) age, 2) gender, 3) sampling location of the ocular and periocular surfaces, and 4) topical drop use. 16s sequencing was performed using Illumina MiSeq 250 and analyzed using Qiime.Results: Thirty patients (15 children [mean 3.7 years], 15 adults [mean 60.4 years]) were sampled. Both principal coordinate analysis and unifrac distance analysis showed significant differences in the composition between the pediatric and adult OSMs (both p=0.001). The eyelid margin microbiota did not show any distinct clustering compared to conjunctiva within the pediatric samples but tended to show a distinction between anatomic sites in adult samples. No differences in OSM were noted by topical drop use.Conclusion: 16s sequencing is a useful tool in evaluating the OSM in patients of all ages, showing a distinct difference between pediatric and adult microbiomes. Keywords: ocular microbiome, conjunctival microbiome, eyelid margin microbiome, skin microbiome, 16s rRNA, paucibacterial, QiimeCavuoto KMMendez RMiller DGalor ABanerjee SDove Medical PressarticleOcular microbiomeconjunctival microbiomeeyelid margin microbiomeskin microbiome16s rRNAchildrenadultOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 12, Pp 1189-1197 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ocular microbiome conjunctival microbiome eyelid margin microbiome skin microbiome 16s rRNA children adult Ophthalmology RE1-994
spellingShingle	Ocular microbiome conjunctival microbiome eyelid margin microbiome skin microbiome 16s rRNA children adult Ophthalmology RE1-994 Cavuoto KM Mendez R Miller D Galor A Banerjee S Effect of clinical parameters on the ocular surface microbiome in children and adults
description	Kara M Cavuoto,1,* Roberto Mendez,2,* Darlene Miller,1 Anat Galor,1,3 Santanu Banerjee2 1Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami, Miami, FL, 2Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL, 3Department of Ophthalmology, Miami Veterans Administration Medical Center, Miami, FL, USA *These authors contributed equally to this work Purpose: To perform a pilot study to characterize the effect of clinical parameters on the ocular surface microbiome (OSM) in children and adults using 16s ribosomal RNA sequencing.Methods: Prospective, cross-sectional study using 16s sequencing to evaluate the OSM. Comparisons were made in bacterial composition by 1) age, 2) gender, 3) sampling location of the ocular and periocular surfaces, and 4) topical drop use. 16s sequencing was performed using Illumina MiSeq 250 and analyzed using Qiime.Results: Thirty patients (15 children [mean 3.7 years], 15 adults [mean 60.4 years]) were sampled. Both principal coordinate analysis and unifrac distance analysis showed significant differences in the composition between the pediatric and adult OSMs (both p=0.001). The eyelid margin microbiota did not show any distinct clustering compared to conjunctiva within the pediatric samples but tended to show a distinction between anatomic sites in adult samples. No differences in OSM were noted by topical drop use.Conclusion: 16s sequencing is a useful tool in evaluating the OSM in patients of all ages, showing a distinct difference between pediatric and adult microbiomes. Keywords: ocular microbiome, conjunctival microbiome, eyelid margin microbiome, skin microbiome, 16s rRNA, paucibacterial, Qiime
format	article
author	Cavuoto KM Mendez R Miller D Galor A Banerjee S
author_facet	Cavuoto KM Mendez R Miller D Galor A Banerjee S
author_sort	Cavuoto KM
title	Effect of clinical parameters on the ocular surface microbiome in children and adults
title_short	Effect of clinical parameters on the ocular surface microbiome in children and adults
title_full	Effect of clinical parameters on the ocular surface microbiome in children and adults
title_fullStr	Effect of clinical parameters on the ocular surface microbiome in children and adults
title_full_unstemmed	Effect of clinical parameters on the ocular surface microbiome in children and adults
title_sort	effect of clinical parameters on the ocular surface microbiome in children and adults
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/eccefdc53d4642389a4dabfd3a5d3a09
work_keys_str_mv	AT cavuotokm effectofclinicalparametersontheocularsurfacemicrobiomeinchildrenandadults AT mendezr effectofclinicalparametersontheocularsurfacemicrobiomeinchildrenandadults AT millerd effectofclinicalparametersontheocularsurfacemicrobiomeinchildrenandadults AT galora effectofclinicalparametersontheocularsurfacemicrobiomeinchildrenandadults AT banerjees effectofclinicalparametersontheocularsurfacemicrobiomeinchildrenandadults
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Effect of clinical parameters on the ocular surface microbiome in children and adults

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