Reduced Beige Adipogenic Potential in Subcutaneous Adipocytes Derived from Obese Chinese Individuals

Han Li,1,* Lin Shen,2,* Lei Zhang,3 Bing Yan,3 Tao Sun,3 Feng Guo,3 Xiao Yin1 1Department of Endocrinology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, People’s Republic of China; 2Shandong First Medical University, Taian, Shandong 271000, People&r...

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Autores principales:	Li H, Shen L, Zhang L, Yan B, Sun T, Guo F, Yin X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	obesity beige adipocyte subcutaneous fat chinese Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/eccf1719495841ac9f7f44764327b102
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spelling	oai:doaj.org-article:eccf1719495841ac9f7f44764327b1022021-12-02T13:11:04ZReduced Beige Adipogenic Potential in Subcutaneous Adipocytes Derived from Obese Chinese Individuals1178-7007https://doaj.org/article/eccf1719495841ac9f7f44764327b1022020-07-01T00:00:00Zhttps://www.dovepress.com/reduced-beige-adipogenic-potential-in-subcutaneous-adipocytes-derived--peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Han Li,1,* Lin Shen,2,* Lei Zhang,3 Bing Yan,3 Tao Sun,3 Feng Guo,3 Xiao Yin1 1Department of Endocrinology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, People’s Republic of China; 2Shandong First Medical University, Taian, Shandong 271000, People’s Republic of China; 3Department of Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiao Yin Email yinxiao@sdu.edu.cnPurpose: Thermogenesis function has made brown/beige adipocyte an attractive target for obesity. Human brown adipose tissue activity is impaired in obesity in vivo. The present study aims to compare the differences in beige adipocyte differentiation potential of subcutaneous adipose tissue derived from normal weight and obese Chinese individuals in vitro.Methods: Adipose-derived stem cells (ADSCs) isolated from subcutaneous fat tissues of normal weight (NW) and obese (OB) groups were induced to differentiate into mature adipocyte with white adipocyte (WA)- and beige adipocyte (BA)-induction treatment. The expression of beige adipocyte marker protein UCP-1 and specific thermogenic genes was detected in differentiated adipocytes via Western blot and rt PCR, and the adipocyte mitochondrial function and lipolysis ability were also measured by oxygen consumption rate (OCR) and glycerol release rate, respectively.Results: Either with WA-induction or BA-induction, the expression of UCP-1 and beige adipocyte-specific thermogenic genes in differentiated adipocytes was higher in the NW compared to the OB group, followed by higher OCR and lipolysis ability in NW group than OB group. With BA-induction, expression of UCP-1 and thermogenic genes increased significantly, followed by the increasement in adipocytes OCR and lipolysis rate in NW group compared with WA-induction treatment, but no significant difference was observed in OB group.Conclusion: Compromised beige adipocyte differentiation plasticity was found in subcutaneous white adipose tissue derived from obese Chinese individuals, which may be due part to the downregulation of β 3-adrenergic receptor expression in adipocytes. Discovery of therapeutic agents to active brown adipose tissue through specific pathways could provide a promising approach for treating obesity in the future.Keywords: obesity, beige adipocyte, subcutaneous fat, ChineseLi HShen LZhang LYan BSun TGuo FYin XDove Medical Pressarticleobesitybeige adipocytesubcutaneous fatchineseSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 2551-2562 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	obesity beige adipocyte subcutaneous fat chinese Specialties of internal medicine RC581-951
spellingShingle	obesity beige adipocyte subcutaneous fat chinese Specialties of internal medicine RC581-951 Li H Shen L Zhang L Yan B Sun T Guo F Yin X Reduced Beige Adipogenic Potential in Subcutaneous Adipocytes Derived from Obese Chinese Individuals
description	Han Li,1,* Lin Shen,2,* Lei Zhang,3 Bing Yan,3 Tao Sun,3 Feng Guo,3 Xiao Yin1 1Department of Endocrinology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, People’s Republic of China; 2Shandong First Medical University, Taian, Shandong 271000, People’s Republic of China; 3Department of Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong 250013, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiao Yin Email yinxiao@sdu.edu.cnPurpose: Thermogenesis function has made brown/beige adipocyte an attractive target for obesity. Human brown adipose tissue activity is impaired in obesity in vivo. The present study aims to compare the differences in beige adipocyte differentiation potential of subcutaneous adipose tissue derived from normal weight and obese Chinese individuals in vitro.Methods: Adipose-derived stem cells (ADSCs) isolated from subcutaneous fat tissues of normal weight (NW) and obese (OB) groups were induced to differentiate into mature adipocyte with white adipocyte (WA)- and beige adipocyte (BA)-induction treatment. The expression of beige adipocyte marker protein UCP-1 and specific thermogenic genes was detected in differentiated adipocytes via Western blot and rt PCR, and the adipocyte mitochondrial function and lipolysis ability were also measured by oxygen consumption rate (OCR) and glycerol release rate, respectively.Results: Either with WA-induction or BA-induction, the expression of UCP-1 and beige adipocyte-specific thermogenic genes in differentiated adipocytes was higher in the NW compared to the OB group, followed by higher OCR and lipolysis ability in NW group than OB group. With BA-induction, expression of UCP-1 and thermogenic genes increased significantly, followed by the increasement in adipocytes OCR and lipolysis rate in NW group compared with WA-induction treatment, but no significant difference was observed in OB group.Conclusion: Compromised beige adipocyte differentiation plasticity was found in subcutaneous white adipose tissue derived from obese Chinese individuals, which may be due part to the downregulation of β 3-adrenergic receptor expression in adipocytes. Discovery of therapeutic agents to active brown adipose tissue through specific pathways could provide a promising approach for treating obesity in the future.Keywords: obesity, beige adipocyte, subcutaneous fat, Chinese
format	article
author	Li H Shen L Zhang L Yan B Sun T Guo F Yin X
author_facet	Li H Shen L Zhang L Yan B Sun T Guo F Yin X
author_sort	Li H
title	Reduced Beige Adipogenic Potential in Subcutaneous Adipocytes Derived from Obese Chinese Individuals
title_short	Reduced Beige Adipogenic Potential in Subcutaneous Adipocytes Derived from Obese Chinese Individuals
title_full	Reduced Beige Adipogenic Potential in Subcutaneous Adipocytes Derived from Obese Chinese Individuals
title_fullStr	Reduced Beige Adipogenic Potential in Subcutaneous Adipocytes Derived from Obese Chinese Individuals
title_full_unstemmed	Reduced Beige Adipogenic Potential in Subcutaneous Adipocytes Derived from Obese Chinese Individuals
title_sort	reduced beige adipogenic potential in subcutaneous adipocytes derived from obese chinese individuals
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/eccf1719495841ac9f7f44764327b102
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Reduced Beige Adipogenic Potential in Subcutaneous Adipocytes Derived from Obese Chinese Individuals

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