In vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome.

<h4>Background</h4>The current understanding of the functional characteristics of circulating endothelial progenitor cells (EPC) is limited, especially in patients affected by cardiovascular diseases. In this study, we have analyzed the in vitro clonogenic capacity of circulating EPC, al...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Diana Campioni, Giorgio Zauli, Stefania Gambetti, Gianluca Campo, Antonio Cuneo, Roberto Ferrari, Paola Secchiero
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ece3a50618924c04910f4f9b2904c8a6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ece3a50618924c04910f4f9b2904c8a6
record_format	dspace
spelling	oai:doaj.org-article:ece3a50618924c04910f4f9b2904c8a62021-11-18T07:58:00ZIn vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome.1932-620310.1371/journal.pone.0056377https://doaj.org/article/ece3a50618924c04910f4f9b2904c8a62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23409178/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The current understanding of the functional characteristics of circulating endothelial progenitor cells (EPC) is limited, especially in patients affected by cardiovascular diseases. In this study, we have analyzed the in vitro clonogenic capacity of circulating EPC, also known as endothelial colony-forming cells (ECFC), in patients with acute coronary syndrome (ACS), in comparison to the colony forming unit-endothelial-like cells (CFU-EC) of hematopoietic/monocytic origin.<h4>Methodology/principal findings</h4>By culturing peripheral blood mononuclear cells (PBMC) of patients with ACS (n = 70), CFU-EC were frequently isolated (from 77% of ACS patients), while EPC/ECFC were obtained only in a small subset (13%) of PBMC samples, all harvested between 7-14 days after the acute cardiovascular event. Notably, ex-vivo generation of EPC/ECFC was correlated to a higher in vitro release of PDGF-AA by the corresponding ACS patient PBMC. By using specific endothelial culture media, EPC/ECFC displayed in vitro expansion capacity, allowing the phenotypic and functional characterization of the cells. Indeed, after expansion, EPC/ECFC exhibited a normal diploid chromosomal setting by FISH analysis and an immunophenotype characterized by: i) uniform positivity for the expression of CD105, CD31, CD146 and Factor VIII, i) variable expression of the CD34, CD106 and CD184 markers, and iii) negativity for CD45, CD90, CD117 and CD133. Of interest, in single-cell replanting assays EPC/ECFC exhibited clonogenic expansion capacity, forming secondary colonies characterized by variable proliferation capacities.<h4>Conclusion/significance</h4>Our data indicate that a careful characterization of true EPC is needed in order to design future studies in the clinical autologous setting of patients with ACS.Diana CampioniGiorgio ZauliStefania GambettiGianluca CampoAntonio CuneoRoberto FerrariPaola SecchieroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e56377 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diana Campioni Giorgio Zauli Stefania Gambetti Gianluca Campo Antonio Cuneo Roberto Ferrari Paola Secchiero In vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome.
description	<h4>Background</h4>The current understanding of the functional characteristics of circulating endothelial progenitor cells (EPC) is limited, especially in patients affected by cardiovascular diseases. In this study, we have analyzed the in vitro clonogenic capacity of circulating EPC, also known as endothelial colony-forming cells (ECFC), in patients with acute coronary syndrome (ACS), in comparison to the colony forming unit-endothelial-like cells (CFU-EC) of hematopoietic/monocytic origin.<h4>Methodology/principal findings</h4>By culturing peripheral blood mononuclear cells (PBMC) of patients with ACS (n = 70), CFU-EC were frequently isolated (from 77% of ACS patients), while EPC/ECFC were obtained only in a small subset (13%) of PBMC samples, all harvested between 7-14 days after the acute cardiovascular event. Notably, ex-vivo generation of EPC/ECFC was correlated to a higher in vitro release of PDGF-AA by the corresponding ACS patient PBMC. By using specific endothelial culture media, EPC/ECFC displayed in vitro expansion capacity, allowing the phenotypic and functional characterization of the cells. Indeed, after expansion, EPC/ECFC exhibited a normal diploid chromosomal setting by FISH analysis and an immunophenotype characterized by: i) uniform positivity for the expression of CD105, CD31, CD146 and Factor VIII, i) variable expression of the CD34, CD106 and CD184 markers, and iii) negativity for CD45, CD90, CD117 and CD133. Of interest, in single-cell replanting assays EPC/ECFC exhibited clonogenic expansion capacity, forming secondary colonies characterized by variable proliferation capacities.<h4>Conclusion/significance</h4>Our data indicate that a careful characterization of true EPC is needed in order to design future studies in the clinical autologous setting of patients with ACS.
format	article
author	Diana Campioni Giorgio Zauli Stefania Gambetti Gianluca Campo Antonio Cuneo Roberto Ferrari Paola Secchiero
author_facet	Diana Campioni Giorgio Zauli Stefania Gambetti Gianluca Campo Antonio Cuneo Roberto Ferrari Paola Secchiero
author_sort	Diana Campioni
title	In vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome.
title_short	In vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome.
title_full	In vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome.
title_fullStr	In vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome.
title_full_unstemmed	In vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome.
title_sort	in vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ece3a50618924c04910f4f9b2904c8a6
work_keys_str_mv	AT dianacampioni invitrocharacterizationofcirculatingendothelialprogenitorcellsisolatedfrompatientswithacutecoronarysyndrome AT giorgiozauli invitrocharacterizationofcirculatingendothelialprogenitorcellsisolatedfrompatientswithacutecoronarysyndrome AT stefaniagambetti invitrocharacterizationofcirculatingendothelialprogenitorcellsisolatedfrompatientswithacutecoronarysyndrome AT gianlucacampo invitrocharacterizationofcirculatingendothelialprogenitorcellsisolatedfrompatientswithacutecoronarysyndrome AT antoniocuneo invitrocharacterizationofcirculatingendothelialprogenitorcellsisolatedfrompatientswithacutecoronarysyndrome AT robertoferrari invitrocharacterizationofcirculatingendothelialprogenitorcellsisolatedfrompatientswithacutecoronarysyndrome AT paolasecchiero invitrocharacterizationofcirculatingendothelialprogenitorcellsisolatedfrompatientswithacutecoronarysyndrome
_version_	1718422765410713600

In vitro characterization of circulating endothelial progenitor cells isolated from patients with acute coronary syndrome.

Ejemplares similares