Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia

Abstract. Wibisono FJ, Sumiarto B, Untari T, Effendi MH, Permatasari DA, Witaningrum AM. 2020. Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia. Biodiversitas 21: 4631-4635. The aims of thi...

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Autores principales:	FRESHINTA JELLIA WIBISONO, BAMBANG SUMIARTO, TRI UNTARI, Mustofa Helmi Effendi, DIAN AYU PERMATASARI, ADIANA MUTAMSARI WITANINGRUM
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	antimicrobial resistance esbl genes escherichia coli food safety Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ece68201aa37482d97cf78ca58af69c7
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spelling	oai:doaj.org-article:ece68201aa37482d97cf78ca58af69c72021-11-22T00:51:09ZShort Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia1412-033X2085-472210.13057/biodiv/d211022https://doaj.org/article/ece68201aa37482d97cf78ca58af69c72020-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6483https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Wibisono FJ, Sumiarto B, Untari T, Effendi MH, Permatasari DA, Witaningrum AM. 2020. Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia. Biodiversitas 21: 4631-4635. The aims of this study were to determine the susceptibility pattern of phenotypic antibiotics on extended-spectrum beta-lactamases (ESBL) genes and genotype profiles of ESBL producing Escherichia coli strains isolated from cloacal samples of laying hens in Blitar. A total of 165 cloacal swab samples were successfully isolated 145 E. coli strains during the study taken from 5 subdistricts in Blitar. All the strains were examined for antibiotic resistance patterns by disk diffusion method with double-disk synergy test (DDST), followed testing with VITEK® 2 methods, molecular identification of ESBL coding genes using PCR. The results of this study showed that the characterization of nucleotide analysis from PCR amplification of ESBL-producing E. coli bacteria isolated from laying hens in Blitar showed that eight isolates were the dominant of CTX gene, followed by the TEM encoding gene of two isolates, and the SHV coding gene as much as one isolate. The presence of more than 1 encoding genes in the E. coli bacterial isolate was seen in 1 isolate, where the isolate carried the CTX gene and the SHV gene as well. All ESBL producing E. coli isolates were resistant to amoxicillin, ampicillin, cefazolin, cefotaxime, and ceftriaxone, and these ESBL isolates were more than 70% resistant to gentamicin, aztreonam, and trimethoprim/sulfamethoxazole. These results indicated that poultry is a potential reservoir for ESBL-producing E. coli. The presence of ESBL-producing E. coli in poultry requires strengthening antibiotic policy. This is important because the regulation of antibiotic use in poultry is gaining momentum to increase animal productivity and food safety in Blitar, Indonesia.FRESHINTA JELLIA WIBISONOBAMBANG SUMIARTOTRI UNTARIMustofa Helmi EffendiDIAN AYU PERMATASARIADIANA MUTAMSARI WITANINGRUMMBI & UNS Soloarticleantimicrobial resistanceesbl genesescherichia colifood safetyBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial resistance esbl genes escherichia coli food safety Biology (General) QH301-705.5
spellingShingle	antimicrobial resistance esbl genes escherichia coli food safety Biology (General) QH301-705.5 FRESHINTA JELLIA WIBISONO BAMBANG SUMIARTO TRI UNTARI Mustofa Helmi Effendi DIAN AYU PERMATASARI ADIANA MUTAMSARI WITANINGRUM Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia
description	Abstract. Wibisono FJ, Sumiarto B, Untari T, Effendi MH, Permatasari DA, Witaningrum AM. 2020. Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia. Biodiversitas 21: 4631-4635. The aims of this study were to determine the susceptibility pattern of phenotypic antibiotics on extended-spectrum beta-lactamases (ESBL) genes and genotype profiles of ESBL producing Escherichia coli strains isolated from cloacal samples of laying hens in Blitar. A total of 165 cloacal swab samples were successfully isolated 145 E. coli strains during the study taken from 5 subdistricts in Blitar. All the strains were examined for antibiotic resistance patterns by disk diffusion method with double-disk synergy test (DDST), followed testing with VITEK® 2 methods, molecular identification of ESBL coding genes using PCR. The results of this study showed that the characterization of nucleotide analysis from PCR amplification of ESBL-producing E. coli bacteria isolated from laying hens in Blitar showed that eight isolates were the dominant of CTX gene, followed by the TEM encoding gene of two isolates, and the SHV coding gene as much as one isolate. The presence of more than 1 encoding genes in the E. coli bacterial isolate was seen in 1 isolate, where the isolate carried the CTX gene and the SHV gene as well. All ESBL producing E. coli isolates were resistant to amoxicillin, ampicillin, cefazolin, cefotaxime, and ceftriaxone, and these ESBL isolates were more than 70% resistant to gentamicin, aztreonam, and trimethoprim/sulfamethoxazole. These results indicated that poultry is a potential reservoir for ESBL-producing E. coli. The presence of ESBL-producing E. coli in poultry requires strengthening antibiotic policy. This is important because the regulation of antibiotic use in poultry is gaining momentum to increase animal productivity and food safety in Blitar, Indonesia.
format	article
author	FRESHINTA JELLIA WIBISONO BAMBANG SUMIARTO TRI UNTARI Mustofa Helmi Effendi DIAN AYU PERMATASARI ADIANA MUTAMSARI WITANINGRUM
author_facet	FRESHINTA JELLIA WIBISONO BAMBANG SUMIARTO TRI UNTARI Mustofa Helmi Effendi DIAN AYU PERMATASARI ADIANA MUTAMSARI WITANINGRUM
author_sort	FRESHINTA JELLIA WIBISONO
title	Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia
title_short	Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia
title_full	Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia
title_fullStr	Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia
title_full_unstemmed	Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia
title_sort	short communication: pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing escherichia coli on laying hens in blitar, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/ece68201aa37482d97cf78ca58af69c7
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Short Communication: Pattern of antibiotic resistance on extended-spectrum beta-lactamases genes producing Escherichia coli on laying hens in Blitar, Indonesia

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