Epistatic QTLsPlay a Major Role in Nitrogen Use Efficiency and Its Component Traits in Indian Spring Wheat

Nitrogen use efficiency (NUE) in wheat may significantly reduce the excessive use of N fertilizers. However, being a quantitative trait, understanding its genetic basis is required for efficient wheat breeding. The present study was carried out to dissect the complex trait through the mapping of qua...

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Autores principales:	Rumesh Ranjan, Rajbir Yadav, Neelu Jain, Nivedita Sinha, Naresh Kumar Bainsla, Kiran B. Gaikwad, Manjeet Kumar
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	QTL mapping epistasis root dry weight nitrogen use efficiency wheat Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/ece97626f8a6476aba308e302d29adc4
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id	oai:doaj.org-article:ece97626f8a6476aba308e302d29adc4
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spelling	oai:doaj.org-article:ece97626f8a6476aba308e302d29adc42021-11-25T16:00:48ZEpistatic QTLsPlay a Major Role in Nitrogen Use Efficiency and Its Component Traits in Indian Spring Wheat10.3390/agriculture111111492077-0472https://doaj.org/article/ece97626f8a6476aba308e302d29adc42021-11-01T00:00:00Zhttps://www.mdpi.com/2077-0472/11/11/1149https://doaj.org/toc/2077-0472Nitrogen use efficiency (NUE) in wheat may significantly reduce the excessive use of N fertilizers. However, being a quantitative trait, understanding its genetic basis is required for efficient wheat breeding. The present study was carried out to dissect the complex trait through the mapping of quantitative trait locus (QTLs) related to NUE component traits in Indian wheat. A linkage map was constructed using F<sub>2</sub> population derived from two parents contrasting for nitrogen-responsive traits using simple sequence repeat (SSR) markers. Phenotyping for root dry weight, N uptake and utilization were carried out under a high nitrogen environment. Twenty-seven main effect QTLs for eight traits and 26 interaction QTLs for three traits were detected. The main effect QTLs explained a significant amount of phenotypic variance up to the extent of 11.18%. The QTLs were also found to have significant epistatic interactions governed by both additive and non-additive gene action. In particular, chromosome 2A harbours QTLs for many traits <i>viz.</i> SDW, RDW, TDW, R:S, %N, NUtE, and NUpE, including epistasis and interaction QTLs that were flanked by markers X<i>wmc</i>728-X<i>wmc</i>473 and X<i>wmc</i>779-X<i>gwm</i>249. Taken together, the genomic regions on 2A, 4A, and 7A were found to contain QTLs for a majority of the studied NUE traits that can be potentially exploited in future wheat breeding programmes.Rumesh RanjanRajbir YadavNeelu JainNivedita SinhaNaresh Kumar BainslaKiran B. GaikwadManjeet KumarMDPI AGarticleQTL mappingepistasisroot dry weightnitrogen use efficiencywheatAgriculture (General)S1-972ENAgriculture, Vol 11, Iss 1149, p 1149 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	QTL mapping epistasis root dry weight nitrogen use efficiency wheat Agriculture (General) S1-972
spellingShingle	QTL mapping epistasis root dry weight nitrogen use efficiency wheat Agriculture (General) S1-972 Rumesh Ranjan Rajbir Yadav Neelu Jain Nivedita Sinha Naresh Kumar Bainsla Kiran B. Gaikwad Manjeet Kumar Epistatic QTLsPlay a Major Role in Nitrogen Use Efficiency and Its Component Traits in Indian Spring Wheat
description	Nitrogen use efficiency (NUE) in wheat may significantly reduce the excessive use of N fertilizers. However, being a quantitative trait, understanding its genetic basis is required for efficient wheat breeding. The present study was carried out to dissect the complex trait through the mapping of quantitative trait locus (QTLs) related to NUE component traits in Indian wheat. A linkage map was constructed using F<sub>2</sub> population derived from two parents contrasting for nitrogen-responsive traits using simple sequence repeat (SSR) markers. Phenotyping for root dry weight, N uptake and utilization were carried out under a high nitrogen environment. Twenty-seven main effect QTLs for eight traits and 26 interaction QTLs for three traits were detected. The main effect QTLs explained a significant amount of phenotypic variance up to the extent of 11.18%. The QTLs were also found to have significant epistatic interactions governed by both additive and non-additive gene action. In particular, chromosome 2A harbours QTLs for many traits <i>viz.</i> SDW, RDW, TDW, R:S, %N, NUtE, and NUpE, including epistasis and interaction QTLs that were flanked by markers X<i>wmc</i>728-X<i>wmc</i>473 and X<i>wmc</i>779-X<i>gwm</i>249. Taken together, the genomic regions on 2A, 4A, and 7A were found to contain QTLs for a majority of the studied NUE traits that can be potentially exploited in future wheat breeding programmes.
format	article
author	Rumesh Ranjan Rajbir Yadav Neelu Jain Nivedita Sinha Naresh Kumar Bainsla Kiran B. Gaikwad Manjeet Kumar
author_facet	Rumesh Ranjan Rajbir Yadav Neelu Jain Nivedita Sinha Naresh Kumar Bainsla Kiran B. Gaikwad Manjeet Kumar
author_sort	Rumesh Ranjan
title	Epistatic QTLsPlay a Major Role in Nitrogen Use Efficiency and Its Component Traits in Indian Spring Wheat
title_short	Epistatic QTLsPlay a Major Role in Nitrogen Use Efficiency and Its Component Traits in Indian Spring Wheat
title_full	Epistatic QTLsPlay a Major Role in Nitrogen Use Efficiency and Its Component Traits in Indian Spring Wheat
title_fullStr	Epistatic QTLsPlay a Major Role in Nitrogen Use Efficiency and Its Component Traits in Indian Spring Wheat
title_full_unstemmed	Epistatic QTLsPlay a Major Role in Nitrogen Use Efficiency and Its Component Traits in Indian Spring Wheat
title_sort	epistatic qtlsplay a major role in nitrogen use efficiency and its component traits in indian spring wheat
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ece97626f8a6476aba308e302d29adc4
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Epistatic QTLsPlay a Major Role in Nitrogen Use Efficiency and Its Component Traits in Indian Spring Wheat

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