Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling

Abstract Cells mechanical property assessment has been a promising label-free method for cell differentiation. Several methods have been proposed for single-cell mechanical properties analysis. Dielectrophoresis (DEP) is one method used for single-cell mechanical property assessment, cell separation...

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Autores principales:	Imman I. Hosseini, Mahdi Moghimi Zand, Amir Ali Ebadi, Morteza Fathipour
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ecf1c1a308a047fcb620ca7d9c13251c
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spelling	oai:doaj.org-article:ecf1c1a308a047fcb620ca7d9c13251c2021-12-02T13:57:25ZCell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling10.1038/s41598-020-78411-12045-2322https://doaj.org/article/ecf1c1a308a047fcb620ca7d9c13251c2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78411-1https://doaj.org/toc/2045-2322Abstract Cells mechanical property assessment has been a promising label-free method for cell differentiation. Several methods have been proposed for single-cell mechanical properties analysis. Dielectrophoresis (DEP) is one method used for single-cell mechanical property assessment, cell separation, and sorting. DEP method has overcome weaknesses of other techniques, including compatibility with microfluidics, high throughput assessment, and high accuracy. However, due to the lack of a general and explicit model for this method, it has not been known as an ideal cell mechanical property evaluation method. Here we present an explicit model using the most general electromagnetic equation (Maxwell Stress Tensor) for single-cell mechanical evaluation based on the DEP method. For proof of concept, we used the proposed model for differentiation between three different types of cells, namely erythrocytes, peripheral blood mononuclear cells (PBMC), and an epithelial breast cancer cells line (T-47D). The results show that, by a lumped parameter that depends on cells' mechanical and electrical properties, the proposed model can successfully distinguish between the mentioned cell types that can be in a single blood sample. The proposed model would open up the chance to use a mechanical assessment method for cell searching in parallel with other methods.Imman I. HosseiniMahdi Moghimi ZandAmir Ali EbadiMorteza FathipourNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Imman I. Hosseini Mahdi Moghimi Zand Amir Ali Ebadi Morteza Fathipour Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
description	Abstract Cells mechanical property assessment has been a promising label-free method for cell differentiation. Several methods have been proposed for single-cell mechanical properties analysis. Dielectrophoresis (DEP) is one method used for single-cell mechanical property assessment, cell separation, and sorting. DEP method has overcome weaknesses of other techniques, including compatibility with microfluidics, high throughput assessment, and high accuracy. However, due to the lack of a general and explicit model for this method, it has not been known as an ideal cell mechanical property evaluation method. Here we present an explicit model using the most general electromagnetic equation (Maxwell Stress Tensor) for single-cell mechanical evaluation based on the DEP method. For proof of concept, we used the proposed model for differentiation between three different types of cells, namely erythrocytes, peripheral blood mononuclear cells (PBMC), and an epithelial breast cancer cells line (T-47D). The results show that, by a lumped parameter that depends on cells' mechanical and electrical properties, the proposed model can successfully distinguish between the mentioned cell types that can be in a single blood sample. The proposed model would open up the chance to use a mechanical assessment method for cell searching in parallel with other methods.
format	article
author	Imman I. Hosseini Mahdi Moghimi Zand Amir Ali Ebadi Morteza Fathipour
author_facet	Imman I. Hosseini Mahdi Moghimi Zand Amir Ali Ebadi Morteza Fathipour
author_sort	Imman I. Hosseini
title	Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
title_short	Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
title_full	Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
title_fullStr	Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
title_full_unstemmed	Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
title_sort	cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ecf1c1a308a047fcb620ca7d9c13251c
work_keys_str_mv	AT immanihosseini cellpropertiesassessmentusingoptimizeddielectrophoresisbasedcellstretchingandlumpedmechanicalmodeling AT mahdimoghimizand cellpropertiesassessmentusingoptimizeddielectrophoresisbasedcellstretchingandlumpedmechanicalmodeling AT amiraliebadi cellpropertiesassessmentusingoptimizeddielectrophoresisbasedcellstretchingandlumpedmechanicalmodeling AT mortezafathipour cellpropertiesassessmentusingoptimizeddielectrophoresisbasedcellstretchingandlumpedmechanicalmodeling
_version_	1718392355714760704

Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling

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