Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling

Abstract Cells mechanical property assessment has been a promising label-free method for cell differentiation. Several methods have been proposed for single-cell mechanical properties analysis. Dielectrophoresis (DEP) is one method used for single-cell mechanical property assessment, cell separation...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Imman I. Hosseini, Mahdi Moghimi Zand, Amir Ali Ebadi, Morteza Fathipour
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
R
Q
Acceso en línea:https://doaj.org/article/ecf1c1a308a047fcb620ca7d9c13251c
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:ecf1c1a308a047fcb620ca7d9c13251c
record_format dspace
spelling oai:doaj.org-article:ecf1c1a308a047fcb620ca7d9c13251c2021-12-02T13:57:25ZCell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling10.1038/s41598-020-78411-12045-2322https://doaj.org/article/ecf1c1a308a047fcb620ca7d9c13251c2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78411-1https://doaj.org/toc/2045-2322Abstract Cells mechanical property assessment has been a promising label-free method for cell differentiation. Several methods have been proposed for single-cell mechanical properties analysis. Dielectrophoresis (DEP) is one method used for single-cell mechanical property assessment, cell separation, and sorting. DEP method has overcome weaknesses of other techniques, including compatibility with microfluidics, high throughput assessment, and high accuracy. However, due to the lack of a general and explicit model for this method, it has not been known as an ideal cell mechanical property evaluation method. Here we present an explicit model using the most general electromagnetic equation (Maxwell Stress Tensor) for single-cell mechanical evaluation based on the DEP method. For proof of concept, we used the proposed model for differentiation between three different types of cells, namely erythrocytes, peripheral blood mononuclear cells (PBMC), and an epithelial breast cancer cells line (T-47D). The results show that, by a lumped parameter that depends on cells' mechanical and electrical properties, the proposed model can successfully distinguish between the mentioned cell types that can be in a single blood sample. The proposed model would open up the chance to use a mechanical assessment method for cell searching in parallel with other methods.Imman I. HosseiniMahdi Moghimi ZandAmir Ali EbadiMorteza FathipourNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Imman I. Hosseini
Mahdi Moghimi Zand
Amir Ali Ebadi
Morteza Fathipour
Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
description Abstract Cells mechanical property assessment has been a promising label-free method for cell differentiation. Several methods have been proposed for single-cell mechanical properties analysis. Dielectrophoresis (DEP) is one method used for single-cell mechanical property assessment, cell separation, and sorting. DEP method has overcome weaknesses of other techniques, including compatibility with microfluidics, high throughput assessment, and high accuracy. However, due to the lack of a general and explicit model for this method, it has not been known as an ideal cell mechanical property evaluation method. Here we present an explicit model using the most general electromagnetic equation (Maxwell Stress Tensor) for single-cell mechanical evaluation based on the DEP method. For proof of concept, we used the proposed model for differentiation between three different types of cells, namely erythrocytes, peripheral blood mononuclear cells (PBMC), and an epithelial breast cancer cells line (T-47D). The results show that, by a lumped parameter that depends on cells' mechanical and electrical properties, the proposed model can successfully distinguish between the mentioned cell types that can be in a single blood sample. The proposed model would open up the chance to use a mechanical assessment method for cell searching in parallel with other methods.
format article
author Imman I. Hosseini
Mahdi Moghimi Zand
Amir Ali Ebadi
Morteza Fathipour
author_facet Imman I. Hosseini
Mahdi Moghimi Zand
Amir Ali Ebadi
Morteza Fathipour
author_sort Imman I. Hosseini
title Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
title_short Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
title_full Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
title_fullStr Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
title_full_unstemmed Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
title_sort cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/ecf1c1a308a047fcb620ca7d9c13251c
work_keys_str_mv AT immanihosseini cellpropertiesassessmentusingoptimizeddielectrophoresisbasedcellstretchingandlumpedmechanicalmodeling
AT mahdimoghimizand cellpropertiesassessmentusingoptimizeddielectrophoresisbasedcellstretchingandlumpedmechanicalmodeling
AT amiraliebadi cellpropertiesassessmentusingoptimizeddielectrophoresisbasedcellstretchingandlumpedmechanicalmodeling
AT mortezafathipour cellpropertiesassessmentusingoptimizeddielectrophoresisbasedcellstretchingandlumpedmechanicalmodeling
_version_ 1718392355714760704