Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection

Yanyan Yu, Gaoxing Su, Hongyan Zhu, Qing Zhu, Yong Chen, Bohui Xu, Yuqin Li, Wei Zhang School of Pharmacy, Nantong University, Nantong, People’s Republic of China Abstract: In this study, we fabricated a novel electrochemical biosensing platform on the basis of target-triggered proximity...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yu Y, Su G, Zhu H, Zhu Q, Chen Y, Xu B, Li Y, Zhang W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Electrochemical biosensor Proximity hybridization PDGF-BB Isothermal exponential amplification G-quadruplex Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ecf2bc84226148769818ff43caa8409e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ecf2bc84226148769818ff43caa8409e
record_format	dspace
spelling	oai:doaj.org-article:ecf2bc84226148769818ff43caa8409e2021-12-02T02:06:52ZProximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection1178-2013https://doaj.org/article/ecf2bc84226148769818ff43caa8409e2017-08-01T00:00:00Zhttps://www.dovepress.com/proximity-hybridization-mediated-isothermal-exponential-amplification--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yanyan Yu, Gaoxing Su, Hongyan Zhu, Qing Zhu, Yong Chen, Bohui Xu, Yuqin Li, Wei Zhang School of Pharmacy, Nantong University, Nantong, People’s Republic of China Abstract: In this study, we fabricated a novel electrochemical biosensing platform on the basis of target-triggered proximity hybridization-mediated isothermal exponential amplification reaction (EXPAR) for ultrasensitive protein analysis. Through rational design, the aptamers for protein recognition were integrated within two DNA probes. Via proximity hybridization principle, the affinity protein-binding event was converted into DNA assembly process. The recognition of protein by aptamers can trigger the strand displacement through the increase of the local concentrations of the involved probes. As a consequence, the output DNA was displaced, which can hybridize with the duplex probes immobilized on the electrode surface subsequently, leading to the initiation of the EXPAR as well as the cleavage of duplex probes. Each cleavage will release the gold nanoparticles (AuNPs) binding sequence. With the modification of G-quadruplex sequence, electrochemical signals were yielded by the AuNPs through oxidizing 3,3',5,5'-tetramethylbenzidine in the presence of H2O2. The study we proposed exhibited high sensitivity toward platelet-derived growth factor BB (PDGF-BB) with the detection limit of 52 fM. And, this method also showed great selectivity among the PDGF isoforms and performed well in spiked human serum samples. Keywords: electrochemical biosensor, proximity hybridization, PDGF-BB, isothermal exponential amplification, G-quadruplex Yu YSu GZhu HZhu QChen YXu BLi YZhang WDove Medical PressarticleElectrochemical biosensorProximity hybridizationPDGF-BBIsothermal exponential amplificationG-quadruplexMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 5903-5914 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Electrochemical biosensor Proximity hybridization PDGF-BB Isothermal exponential amplification G-quadruplex Medicine (General) R5-920
spellingShingle	Electrochemical biosensor Proximity hybridization PDGF-BB Isothermal exponential amplification G-quadruplex Medicine (General) R5-920 Yu Y Su G Zhu H Zhu Q Chen Y Xu B Li Y Zhang W Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection
description	Yanyan Yu, Gaoxing Su, Hongyan Zhu, Qing Zhu, Yong Chen, Bohui Xu, Yuqin Li, Wei Zhang School of Pharmacy, Nantong University, Nantong, People’s Republic of China Abstract: In this study, we fabricated a novel electrochemical biosensing platform on the basis of target-triggered proximity hybridization-mediated isothermal exponential amplification reaction (EXPAR) for ultrasensitive protein analysis. Through rational design, the aptamers for protein recognition were integrated within two DNA probes. Via proximity hybridization principle, the affinity protein-binding event was converted into DNA assembly process. The recognition of protein by aptamers can trigger the strand displacement through the increase of the local concentrations of the involved probes. As a consequence, the output DNA was displaced, which can hybridize with the duplex probes immobilized on the electrode surface subsequently, leading to the initiation of the EXPAR as well as the cleavage of duplex probes. Each cleavage will release the gold nanoparticles (AuNPs) binding sequence. With the modification of G-quadruplex sequence, electrochemical signals were yielded by the AuNPs through oxidizing 3,3',5,5'-tetramethylbenzidine in the presence of H2O2. The study we proposed exhibited high sensitivity toward platelet-derived growth factor BB (PDGF-BB) with the detection limit of 52 fM. And, this method also showed great selectivity among the PDGF isoforms and performed well in spiked human serum samples. Keywords: electrochemical biosensor, proximity hybridization, PDGF-BB, isothermal exponential amplification, G-quadruplex
format	article
author	Yu Y Su G Zhu H Zhu Q Chen Y Xu B Li Y Zhang W
author_facet	Yu Y Su G Zhu H Zhu Q Chen Y Xu B Li Y Zhang W
author_sort	Yu Y
title	Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection
title_short	Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection
title_full	Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection
title_fullStr	Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection
title_full_unstemmed	Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection
title_sort	proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/ecf2bc84226148769818ff43caa8409e
work_keys_str_mv	AT yuy proximityhybridizationmediatedisothermalexponentialamplificationforultrasensitiveelectrochemicalproteindetection AT sug proximityhybridizationmediatedisothermalexponentialamplificationforultrasensitiveelectrochemicalproteindetection AT zhuh proximityhybridizationmediatedisothermalexponentialamplificationforultrasensitiveelectrochemicalproteindetection AT zhuq proximityhybridizationmediatedisothermalexponentialamplificationforultrasensitiveelectrochemicalproteindetection AT cheny proximityhybridizationmediatedisothermalexponentialamplificationforultrasensitiveelectrochemicalproteindetection AT xub proximityhybridizationmediatedisothermalexponentialamplificationforultrasensitiveelectrochemicalproteindetection AT liy proximityhybridizationmediatedisothermalexponentialamplificationforultrasensitiveelectrochemicalproteindetection AT zhangw proximityhybridizationmediatedisothermalexponentialamplificationforultrasensitiveelectrochemicalproteindetection
_version_	1718402746576535552

Proximity hybridization-mediated isothermal exponential amplification for ultrasensitive electrochemical protein detection

Ejemplares similares