Rapid signal enhancement method for nanoprobe-based biosensing

Abstract The introduction of nanomaterials as detection reagents has enabled improved sensitivity and facilitated detection in a variety of bioanalytical assays. However, high nanoprobe densities are typically needed for colorimetric detection and to circumvent this limitation several enhancement pr...

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Autores principales:	Jorge T. Dias, Gustav Svedberg, Mats Nystrand, Helene Andersson-Svahn, Jesper Gantelius
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ecfb0547b3644fdd9fe4ff19a477168a
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spelling	oai:doaj.org-article:ecfb0547b3644fdd9fe4ff19a477168a2021-12-02T12:32:34ZRapid signal enhancement method for nanoprobe-based biosensing10.1038/s41598-017-07030-02045-2322https://doaj.org/article/ecfb0547b3644fdd9fe4ff19a477168a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07030-0https://doaj.org/toc/2045-2322Abstract The introduction of nanomaterials as detection reagents has enabled improved sensitivity and facilitated detection in a variety of bioanalytical assays. However, high nanoprobe densities are typically needed for colorimetric detection and to circumvent this limitation several enhancement protocols have been reported. Nevertheless, there is currently a lack of universal, enzyme-free and versatile methods that can be readily applied to existing as well as new biosensing strategies. The novel method presented here is shown to enhance the signal of gold nanoparticles enabling visual detection of a spot containing <10 nanoparticles. Detection of Protein G on paper arrays was improved by a 100-fold amplification factor in under five minutes of assay time, using IgG-labelled gold, silver, silica and iron oxide nanoprobes. Furthermore, we show that the presented protocol can be applied to a commercial allergen microarray assay, ImmunoCAP ISAC sIgE 112, attaining a good agreement with fluorescent detection when analysing human clinical samples.Jorge T. DiasGustav SvedbergMats NystrandHelene Andersson-SvahnJesper GanteliusNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jorge T. Dias Gustav Svedberg Mats Nystrand Helene Andersson-Svahn Jesper Gantelius Rapid signal enhancement method for nanoprobe-based biosensing
description	Abstract The introduction of nanomaterials as detection reagents has enabled improved sensitivity and facilitated detection in a variety of bioanalytical assays. However, high nanoprobe densities are typically needed for colorimetric detection and to circumvent this limitation several enhancement protocols have been reported. Nevertheless, there is currently a lack of universal, enzyme-free and versatile methods that can be readily applied to existing as well as new biosensing strategies. The novel method presented here is shown to enhance the signal of gold nanoparticles enabling visual detection of a spot containing <10 nanoparticles. Detection of Protein G on paper arrays was improved by a 100-fold amplification factor in under five minutes of assay time, using IgG-labelled gold, silver, silica and iron oxide nanoprobes. Furthermore, we show that the presented protocol can be applied to a commercial allergen microarray assay, ImmunoCAP ISAC sIgE 112, attaining a good agreement with fluorescent detection when analysing human clinical samples.
format	article
author	Jorge T. Dias Gustav Svedberg Mats Nystrand Helene Andersson-Svahn Jesper Gantelius
author_facet	Jorge T. Dias Gustav Svedberg Mats Nystrand Helene Andersson-Svahn Jesper Gantelius
author_sort	Jorge T. Dias
title	Rapid signal enhancement method for nanoprobe-based biosensing
title_short	Rapid signal enhancement method for nanoprobe-based biosensing
title_full	Rapid signal enhancement method for nanoprobe-based biosensing
title_fullStr	Rapid signal enhancement method for nanoprobe-based biosensing
title_full_unstemmed	Rapid signal enhancement method for nanoprobe-based biosensing
title_sort	rapid signal enhancement method for nanoprobe-based biosensing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ecfb0547b3644fdd9fe4ff19a477168a
work_keys_str_mv	AT jorgetdias rapidsignalenhancementmethodfornanoprobebasedbiosensing AT gustavsvedberg rapidsignalenhancementmethodfornanoprobebasedbiosensing AT matsnystrand rapidsignalenhancementmethodfornanoprobebasedbiosensing AT heleneanderssonsvahn rapidsignalenhancementmethodfornanoprobebasedbiosensing AT jespergantelius rapidsignalenhancementmethodfornanoprobebasedbiosensing
_version_	1718394071596138496

Rapid signal enhancement method for nanoprobe-based biosensing

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