The role of innate APOBEC3G and adaptive AID immune responses in HLA-HIV/SIV immunized SHIV infected macaques.

The AID/APOBEC family (activation induced deaminase/apolipoprotein B mRNA editing cytokine deaminase) in B cells play important roles in adaptive and innate immunity. Whereas APOBEC3G has been studied in CD4+ T cells and myeloid cells its functional potential in B cells has received little attention...

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Autores principales: Yufei Wang, Trevor Whittall, Durdana Rahman, Evelien M Bunnik, Robert Vaughan, Jørgen Schøller, Lesley A Bergmeier, David Montefiori, Mahavir Singh, Hanneke Schuitemaker, Thomas Lehner
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Publicado: Public Library of Science (PLoS) 2012
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spelling oai:doaj.org-article:ed06f658a4d04b789928d0a168119e7b2021-11-18T07:22:19ZThe role of innate APOBEC3G and adaptive AID immune responses in HLA-HIV/SIV immunized SHIV infected macaques.1932-620310.1371/journal.pone.0034433https://doaj.org/article/ed06f658a4d04b789928d0a168119e7b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22514633/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The AID/APOBEC family (activation induced deaminase/apolipoprotein B mRNA editing cytokine deaminase) in B cells play important roles in adaptive and innate immunity. Whereas APOBEC3G has been studied in CD4+ T cells and myeloid cells its functional potential in B cells has received little attention. AID combines two critical functions of antibodies, class switching and affinity maturation and may serve as a functional surrogate of protection. These functions were studied following systemic immunization of rhesus macaques with recombinant HLA constructs, linked with HIV and SIV antigens and HSP70 to dextran. The results showed significant upregulation of AID in CD20+ B cells, APOBEC 3G in CD27+ memory B cells and CD4+ effector memory T cells. After immunization the upregulated APOBEC 3G and AID were directly correlated in B cells (p<0.0001). Following challenge with SHIV SF162.P4 the viral load was inversely correlated with AID in B cells and APOBEC 3G in B and T cells, suggesting that both deaminases may have protective functions. Investigation of major interactions between DC, T cells and B cells showed significant increase in membrane associated IL-15 in DC and CD40L in CD4+ T cells. IL-15 binds the IL-15 receptor complex in CD4+ T and B cells, which may reactivate the DC, T and B cell interactions. The overall results are consistent with AID inhibiting pre-entry SHIV by eliciting IgG and IgA antibodies, whereas APOBEC 3G may contribute to the post-entry control of SHIV replication and cellular spread.Yufei WangTrevor WhittallDurdana RahmanEvelien M BunnikRobert VaughanJørgen SchøllerLesley A BergmeierDavid MontefioriMahavir SinghHanneke SchuitemakerThomas LehnerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34433 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yufei Wang
Trevor Whittall
Durdana Rahman
Evelien M Bunnik
Robert Vaughan
Jørgen Schøller
Lesley A Bergmeier
David Montefiori
Mahavir Singh
Hanneke Schuitemaker
Thomas Lehner
The role of innate APOBEC3G and adaptive AID immune responses in HLA-HIV/SIV immunized SHIV infected macaques.
description The AID/APOBEC family (activation induced deaminase/apolipoprotein B mRNA editing cytokine deaminase) in B cells play important roles in adaptive and innate immunity. Whereas APOBEC3G has been studied in CD4+ T cells and myeloid cells its functional potential in B cells has received little attention. AID combines two critical functions of antibodies, class switching and affinity maturation and may serve as a functional surrogate of protection. These functions were studied following systemic immunization of rhesus macaques with recombinant HLA constructs, linked with HIV and SIV antigens and HSP70 to dextran. The results showed significant upregulation of AID in CD20+ B cells, APOBEC 3G in CD27+ memory B cells and CD4+ effector memory T cells. After immunization the upregulated APOBEC 3G and AID were directly correlated in B cells (p<0.0001). Following challenge with SHIV SF162.P4 the viral load was inversely correlated with AID in B cells and APOBEC 3G in B and T cells, suggesting that both deaminases may have protective functions. Investigation of major interactions between DC, T cells and B cells showed significant increase in membrane associated IL-15 in DC and CD40L in CD4+ T cells. IL-15 binds the IL-15 receptor complex in CD4+ T and B cells, which may reactivate the DC, T and B cell interactions. The overall results are consistent with AID inhibiting pre-entry SHIV by eliciting IgG and IgA antibodies, whereas APOBEC 3G may contribute to the post-entry control of SHIV replication and cellular spread.
format article
author Yufei Wang
Trevor Whittall
Durdana Rahman
Evelien M Bunnik
Robert Vaughan
Jørgen Schøller
Lesley A Bergmeier
David Montefiori
Mahavir Singh
Hanneke Schuitemaker
Thomas Lehner
author_facet Yufei Wang
Trevor Whittall
Durdana Rahman
Evelien M Bunnik
Robert Vaughan
Jørgen Schøller
Lesley A Bergmeier
David Montefiori
Mahavir Singh
Hanneke Schuitemaker
Thomas Lehner
author_sort Yufei Wang
title The role of innate APOBEC3G and adaptive AID immune responses in HLA-HIV/SIV immunized SHIV infected macaques.
title_short The role of innate APOBEC3G and adaptive AID immune responses in HLA-HIV/SIV immunized SHIV infected macaques.
title_full The role of innate APOBEC3G and adaptive AID immune responses in HLA-HIV/SIV immunized SHIV infected macaques.
title_fullStr The role of innate APOBEC3G and adaptive AID immune responses in HLA-HIV/SIV immunized SHIV infected macaques.
title_full_unstemmed The role of innate APOBEC3G and adaptive AID immune responses in HLA-HIV/SIV immunized SHIV infected macaques.
title_sort role of innate apobec3g and adaptive aid immune responses in hla-hiv/siv immunized shiv infected macaques.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/ed06f658a4d04b789928d0a168119e7b
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