Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells

Punicalagin, a major ellagitannin isolated from pomegranate, is proved to have various pharmacological activities with an undefined therapy mechanism. The objective of this research was to demonstrate the effect of punicalagin on anti-inflammatory and angiogenic activation in human umbilical vein en...

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Autores principales:	Wei Liu, Yanghui Ou, Yumeng Yang, Xuemei Zhang, Liqi Huang, Xiaohua Wang, Buling Wu, Mingcheng Huang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	punicalagin inflammation endothelial cells angiogenesis NF-κB Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ed14c9e54510419fa29a08e83edfbf3d
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spelling	oai:doaj.org-article:ed14c9e54510419fa29a08e83edfbf3d2021-11-16T07:34:21ZInhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells1663-981210.3389/fphar.2021.727920https://doaj.org/article/ed14c9e54510419fa29a08e83edfbf3d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.727920/fullhttps://doaj.org/toc/1663-9812Punicalagin, a major ellagitannin isolated from pomegranate, is proved to have various pharmacological activities with an undefined therapy mechanism. The objective of this research was to demonstrate the effect of punicalagin on anti-inflammatory and angiogenic activation in human umbilical vein endothelial cells (HUVECs) and their potential mechanisms. Endothelial-leukocyte adhesion assay was applied to evaluate primary cultures of HUVECs activation following tumor necrosis factor alpha (TNF-α) treatment. The endothelial cell proliferation, migration, permeability and tube formation were assessed by EdU assay, wound migration assay, trans-endothelial electrical resistances (TEER) assay, and capillary-like tube formation assay, respectively. In addition, the expression of relevant proteins was assessed using Western blot analysis. We confirmed that punicalagin could reduce the adhesion of human monocyte cells to HUVECs in vitro and in vivo. Further, punicalagin decreased the expression of mRNA and proteins of ICAM-1 and VCAM-1 in HUVECs. Moreover, punicalagin inhibited permeability, proliferation, migration, and tube formation in VEGF-induced HUVECs, suppressed IKK-mediated activation of NF-κB signaling in TNF-α-induced endothelial cells, and inhibited vascular endothelial growth factor receptor 2 (VEGFR2) activation and downstream p-PAK1. Our findings indicated that punicalagin might have a protective effect on HUVECs activation, which suggested that punicalagin functions through an endothelial mediated mechanism for treating various disorders such as, cancer, rheumatoid arthritis, and cardiovascular disease.Wei LiuYanghui OuYumeng YangXuemei ZhangLiqi HuangXiaohua WangBuling WuBuling WuMingcheng HuangFrontiers Media S.A.articlepunicalagininflammationendothelial cellsangiogenesisNF-κBTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	punicalagin inflammation endothelial cells angiogenesis NF-κB Therapeutics. Pharmacology RM1-950
spellingShingle	punicalagin inflammation endothelial cells angiogenesis NF-κB Therapeutics. Pharmacology RM1-950 Wei Liu Yanghui Ou Yumeng Yang Xuemei Zhang Liqi Huang Xiaohua Wang Buling Wu Buling Wu Mingcheng Huang Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells
description	Punicalagin, a major ellagitannin isolated from pomegranate, is proved to have various pharmacological activities with an undefined therapy mechanism. The objective of this research was to demonstrate the effect of punicalagin on anti-inflammatory and angiogenic activation in human umbilical vein endothelial cells (HUVECs) and their potential mechanisms. Endothelial-leukocyte adhesion assay was applied to evaluate primary cultures of HUVECs activation following tumor necrosis factor alpha (TNF-α) treatment. The endothelial cell proliferation, migration, permeability and tube formation were assessed by EdU assay, wound migration assay, trans-endothelial electrical resistances (TEER) assay, and capillary-like tube formation assay, respectively. In addition, the expression of relevant proteins was assessed using Western blot analysis. We confirmed that punicalagin could reduce the adhesion of human monocyte cells to HUVECs in vitro and in vivo. Further, punicalagin decreased the expression of mRNA and proteins of ICAM-1 and VCAM-1 in HUVECs. Moreover, punicalagin inhibited permeability, proliferation, migration, and tube formation in VEGF-induced HUVECs, suppressed IKK-mediated activation of NF-κB signaling in TNF-α-induced endothelial cells, and inhibited vascular endothelial growth factor receptor 2 (VEGFR2) activation and downstream p-PAK1. Our findings indicated that punicalagin might have a protective effect on HUVECs activation, which suggested that punicalagin functions through an endothelial mediated mechanism for treating various disorders such as, cancer, rheumatoid arthritis, and cardiovascular disease.
format	article
author	Wei Liu Yanghui Ou Yumeng Yang Xuemei Zhang Liqi Huang Xiaohua Wang Buling Wu Buling Wu Mingcheng Huang
author_facet	Wei Liu Yanghui Ou Yumeng Yang Xuemei Zhang Liqi Huang Xiaohua Wang Buling Wu Buling Wu Mingcheng Huang
author_sort	Wei Liu
title	Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells
title_short	Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells
title_full	Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells
title_fullStr	Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells
title_full_unstemmed	Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells
title_sort	inhibitory effect of punicalagin on inflammatory and angiogenic activation of human umbilical vein endothelial cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ed14c9e54510419fa29a08e83edfbf3d
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Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells

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