Microgravity inhibits decidualization via decreasing Akt activity and FOXO3a expression in human endometrial stromal cells

Abstract Decidualization is characterized by the differentiation of endometrial stromal cells (eSCs), which is critical for embryo implantation and maintenance of pregnancy. In the present study, we investigated the possible effect of simulated microgravity (SM) on the process of proliferation and i...

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Autores principales:	Hye-Jeong Cho, Mi-Ock Baek, Sana Abdul Khaliq, Seung Joo Chon, Kuk Hui Son, Sung Ho Lee, Mee-Sup Yoon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed19c6a8f0e14d60a5e6b2f4d31f9b39
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spelling	oai:doaj.org-article:ed19c6a8f0e14d60a5e6b2f4d31f9b392021-12-02T15:08:20ZMicrogravity inhibits decidualization via decreasing Akt activity and FOXO3a expression in human endometrial stromal cells10.1038/s41598-019-48580-92045-2322https://doaj.org/article/ed19c6a8f0e14d60a5e6b2f4d31f9b392019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48580-9https://doaj.org/toc/2045-2322Abstract Decidualization is characterized by the differentiation of endometrial stromal cells (eSCs), which is critical for embryo implantation and maintenance of pregnancy. In the present study, we investigated the possible effect of simulated microgravity (SM) on the process of proliferation and in vitro decidualization using primary human eSCs. Exposure to SM for 36 h decreased the proliferation and migration of eSCs significantly, without inducing cell death and changes in cell cycle progression. The phosphorylation of Akt decreased under SM conditions in human eSCs, accompanied by a simultaneous decrease in the level of matrix metalloproteinase (MMP)-2 and FOXO3a. Treatment with Akti, an Akt inhibitor, decreased MMP-2 expression, but not FOXO3a expression. The decreased level of FOXO3a under SM conditions impeded autophagic flux by reducing the levels of autophagy-related genes. In addition, pre-exposure of eSCs to SM significantly inhibited 8-Br-cAMP induced decidualization, whereas restoration of the growth status under SM conditions by removing 8-Br-cAMP remained unchanged. Treatment of human eSCs with SC-79, an Akt activator, restored the reduced migration of eSCs and decidualization under SM conditions. In conclusion, exposure to SM inhibited decidualization in eSCs by decreasing proliferation and migration through Akt/MMP and FOXO3a/autophagic flux.Hye-Jeong ChoMi-Ock BaekSana Abdul KhaliqSeung Joo ChonKuk Hui SonSung Ho LeeMee-Sup YoonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hye-Jeong Cho Mi-Ock Baek Sana Abdul Khaliq Seung Joo Chon Kuk Hui Son Sung Ho Lee Mee-Sup Yoon Microgravity inhibits decidualization via decreasing Akt activity and FOXO3a expression in human endometrial stromal cells
description	Abstract Decidualization is characterized by the differentiation of endometrial stromal cells (eSCs), which is critical for embryo implantation and maintenance of pregnancy. In the present study, we investigated the possible effect of simulated microgravity (SM) on the process of proliferation and in vitro decidualization using primary human eSCs. Exposure to SM for 36 h decreased the proliferation and migration of eSCs significantly, without inducing cell death and changes in cell cycle progression. The phosphorylation of Akt decreased under SM conditions in human eSCs, accompanied by a simultaneous decrease in the level of matrix metalloproteinase (MMP)-2 and FOXO3a. Treatment with Akti, an Akt inhibitor, decreased MMP-2 expression, but not FOXO3a expression. The decreased level of FOXO3a under SM conditions impeded autophagic flux by reducing the levels of autophagy-related genes. In addition, pre-exposure of eSCs to SM significantly inhibited 8-Br-cAMP induced decidualization, whereas restoration of the growth status under SM conditions by removing 8-Br-cAMP remained unchanged. Treatment of human eSCs with SC-79, an Akt activator, restored the reduced migration of eSCs and decidualization under SM conditions. In conclusion, exposure to SM inhibited decidualization in eSCs by decreasing proliferation and migration through Akt/MMP and FOXO3a/autophagic flux.
format	article
author	Hye-Jeong Cho Mi-Ock Baek Sana Abdul Khaliq Seung Joo Chon Kuk Hui Son Sung Ho Lee Mee-Sup Yoon
author_facet	Hye-Jeong Cho Mi-Ock Baek Sana Abdul Khaliq Seung Joo Chon Kuk Hui Son Sung Ho Lee Mee-Sup Yoon
author_sort	Hye-Jeong Cho
title	Microgravity inhibits decidualization via decreasing Akt activity and FOXO3a expression in human endometrial stromal cells
title_short	Microgravity inhibits decidualization via decreasing Akt activity and FOXO3a expression in human endometrial stromal cells
title_full	Microgravity inhibits decidualization via decreasing Akt activity and FOXO3a expression in human endometrial stromal cells
title_fullStr	Microgravity inhibits decidualization via decreasing Akt activity and FOXO3a expression in human endometrial stromal cells
title_full_unstemmed	Microgravity inhibits decidualization via decreasing Akt activity and FOXO3a expression in human endometrial stromal cells
title_sort	microgravity inhibits decidualization via decreasing akt activity and foxo3a expression in human endometrial stromal cells
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ed19c6a8f0e14d60a5e6b2f4d31f9b39
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_version_	1718388225640235008

Microgravity inhibits decidualization via decreasing Akt activity and FOXO3a expression in human endometrial stromal cells

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