Brucella β 1,2 cyclic glucan is an activator of human and mouse dendritic cells.
Bacterial cyclic glucans are glucose polymers that concentrate within the periplasm of alpha-proteobacteria. These molecules are necessary to maintain the homeostasis of the cell envelope by contributing to the osmolarity of Gram negative bacteria. Here, we demonstrate that Brucella β 1,2 cyclic glu...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/ed1affcc153040f3a4afe99dbcf2e92d |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:ed1affcc153040f3a4afe99dbcf2e92d |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:ed1affcc153040f3a4afe99dbcf2e92d2021-11-18T06:06:22ZBrucella β 1,2 cyclic glucan is an activator of human and mouse dendritic cells.1553-73661553-737410.1371/journal.ppat.1002983https://doaj.org/article/ed1affcc153040f3a4afe99dbcf2e92d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23166489/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Bacterial cyclic glucans are glucose polymers that concentrate within the periplasm of alpha-proteobacteria. These molecules are necessary to maintain the homeostasis of the cell envelope by contributing to the osmolarity of Gram negative bacteria. Here, we demonstrate that Brucella β 1,2 cyclic glucans are potent activators of human and mouse dendritic cells. Dendritic cells activation by Brucella β 1,2 cyclic glucans requires TLR4, MyD88 and TRIF, but not CD14. The Brucella cyclic glucans showed neither toxicity nor immunogenicity compared to LPS and triggered antigen-specific CD8(+) T cell responses in vivo. These cyclic glucans also enhanced antigen-specific CD4(+) and CD8(+) T cell responses including cross-presentation by different human DC subsets. Brucella β 1,2 cyclic glucans increased the memory CD4(+) T cell responses of blood mononuclear cells exposed to recombinant fusion proteins composed of anti-CD40 antibody and antigens from both hepatitis C virus and Mycobacterium tuberculosis. Thus cyclic glucans represent a new class of adjuvants, which might contribute to the development of effective antimicrobial therapies.Anna MartirosyanCamino Pérez-GutierrezRomain BanchereauHélène DutartrePatrick LecineMelissa DullaersMarielle MelloSuzana Pinto SalcedoAlexandre MullerLee LesermanYves LevyGerard ZurawskiSandy ZurawskiEdgardo MorenoIgnacio MoriyónEynav KlechevskyJacques BanchereauSangkon OhJean-Pierre GorvelPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 11, p e1002983 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Anna Martirosyan Camino Pérez-Gutierrez Romain Banchereau Hélène Dutartre Patrick Lecine Melissa Dullaers Marielle Mello Suzana Pinto Salcedo Alexandre Muller Lee Leserman Yves Levy Gerard Zurawski Sandy Zurawski Edgardo Moreno Ignacio Moriyón Eynav Klechevsky Jacques Banchereau Sangkon Oh Jean-Pierre Gorvel Brucella β 1,2 cyclic glucan is an activator of human and mouse dendritic cells. |
| description |
Bacterial cyclic glucans are glucose polymers that concentrate within the periplasm of alpha-proteobacteria. These molecules are necessary to maintain the homeostasis of the cell envelope by contributing to the osmolarity of Gram negative bacteria. Here, we demonstrate that Brucella β 1,2 cyclic glucans are potent activators of human and mouse dendritic cells. Dendritic cells activation by Brucella β 1,2 cyclic glucans requires TLR4, MyD88 and TRIF, but not CD14. The Brucella cyclic glucans showed neither toxicity nor immunogenicity compared to LPS and triggered antigen-specific CD8(+) T cell responses in vivo. These cyclic glucans also enhanced antigen-specific CD4(+) and CD8(+) T cell responses including cross-presentation by different human DC subsets. Brucella β 1,2 cyclic glucans increased the memory CD4(+) T cell responses of blood mononuclear cells exposed to recombinant fusion proteins composed of anti-CD40 antibody and antigens from both hepatitis C virus and Mycobacterium tuberculosis. Thus cyclic glucans represent a new class of adjuvants, which might contribute to the development of effective antimicrobial therapies. |
| format |
article |
| author |
Anna Martirosyan Camino Pérez-Gutierrez Romain Banchereau Hélène Dutartre Patrick Lecine Melissa Dullaers Marielle Mello Suzana Pinto Salcedo Alexandre Muller Lee Leserman Yves Levy Gerard Zurawski Sandy Zurawski Edgardo Moreno Ignacio Moriyón Eynav Klechevsky Jacques Banchereau Sangkon Oh Jean-Pierre Gorvel |
| author_facet |
Anna Martirosyan Camino Pérez-Gutierrez Romain Banchereau Hélène Dutartre Patrick Lecine Melissa Dullaers Marielle Mello Suzana Pinto Salcedo Alexandre Muller Lee Leserman Yves Levy Gerard Zurawski Sandy Zurawski Edgardo Moreno Ignacio Moriyón Eynav Klechevsky Jacques Banchereau Sangkon Oh Jean-Pierre Gorvel |
| author_sort |
Anna Martirosyan |
| title |
Brucella β 1,2 cyclic glucan is an activator of human and mouse dendritic cells. |
| title_short |
Brucella β 1,2 cyclic glucan is an activator of human and mouse dendritic cells. |
| title_full |
Brucella β 1,2 cyclic glucan is an activator of human and mouse dendritic cells. |
| title_fullStr |
Brucella β 1,2 cyclic glucan is an activator of human and mouse dendritic cells. |
| title_full_unstemmed |
Brucella β 1,2 cyclic glucan is an activator of human and mouse dendritic cells. |
| title_sort |
brucella β 1,2 cyclic glucan is an activator of human and mouse dendritic cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/ed1affcc153040f3a4afe99dbcf2e92d |
| work_keys_str_mv |
AT annamartirosyan brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT caminoperezgutierrez brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT romainbanchereau brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT helenedutartre brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT patricklecine brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT melissadullaers brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT mariellemello brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT suzanapintosalcedo brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT alexandremuller brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT leeleserman brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT yveslevy brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT gerardzurawski brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT sandyzurawski brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT edgardomoreno brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT ignaciomoriyon brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT eynavklechevsky brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT jacquesbanchereau brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT sangkonoh brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells AT jeanpierregorvel brucellab12cyclicglucanisanactivatorofhumanandmousedendriticcells |
| _version_ |
1718424575372427264 |