Tetracyclines Diminish In Vitro IFN-γ and IL-17-Producing Adaptive and Innate Immune Cells in Multiple Sclerosis

IntroductionLimited data from clinical trials in multiple sclerosis (MS) reported that minocycline, a widely used antibiotic belonging to the family of tetracyclines (TCs), exerts a beneficial short-lived clinical effect A similar anti-inflammatory effect of minocycline attributed to a deviation fro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Despoina T. Florou, Athanasios Mavropoulos, Efthymios Dardiotis, Vana Tsimourtou, Vasileios Siokas, Athina-Maria Aloizou, Christos Liaskos, Christina Tsigalou, Christina Katsiari, Lazaros I. Sakkas, Georgios Hadjigeorgiou, Dimitrios P. Bogdanos
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	multiple sclerosis doxycycline minocycline proinflammatory interferon-γ interleukin-17 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ed1c5b548c154cf3805e7e12acf07aed
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ed1c5b548c154cf3805e7e12acf07aed
record_format	dspace
spelling	oai:doaj.org-article:ed1c5b548c154cf3805e7e12acf07aed2021-12-01T08:18:47ZTetracyclines Diminish In Vitro IFN-γ and IL-17-Producing Adaptive and Innate Immune Cells in Multiple Sclerosis1664-322410.3389/fimmu.2021.739186https://doaj.org/article/ed1c5b548c154cf3805e7e12acf07aed2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.739186/fullhttps://doaj.org/toc/1664-3224IntroductionLimited data from clinical trials in multiple sclerosis (MS) reported that minocycline, a widely used antibiotic belonging to the family of tetracyclines (TCs), exerts a beneficial short-lived clinical effect A similar anti-inflammatory effect of minocycline attributed to a deviation from Th1 to Th2 immune response has been reported in experimental models of MS. Whether such an immunomodulatory mechanism is operated in the human disease remains largely unknown.AimTo assess the in vitro immunomodulatory effect of tetracyclines, and in particular minocycline and doxycycline, in naïve and treated patients with MS.Material and MethodsPeripheral blood mononuclear cells from 45 individuals (35 MS patients, amongst which 15 naïve patients and 10 healthy controls, HCs) were cultured with minocycline or doxycycline and conventional stimulants (PMA/Ionomycin or IL-12/IL-18). IFN-γ and IL-17 producing T-, NK- and NKT cells were assessed by flow cytometry. The effect of TCs on cell viability and apoptosis was further assessed by flow cytometry with Annexin V staining.ResultsBoth tetracyclines significantly decreased, in a dose dependent manner, IFN-γ production in NKT and CD4+ T lymphocytes from MS patients (naïve or treated) stimulated with IL-12/IL-18 but did not decrease IFN-γ producing CD8+ T cells from naive MS or treated RRMS patients. They also decreased IL-17+ T and NKT cells following PMA and Ionomycin-stimulation. Tetracyclines did not affect the viability of cell subsets.ConclusionTetracyclines can in vitro suppress IFN-γ and IL-17- producing cells from MS patients, and this may explain their potential therapeutic effect in vivo.Despoina T. FlorouDespoina T. FlorouAthanasios MavropoulosEfthymios DardiotisVana TsimourtouVasileios SiokasAthina-Maria AloizouChristos LiaskosChristina TsigalouChristina KatsiariLazaros I. SakkasGeorgios HadjigeorgiouGeorgios HadjigeorgiouDimitrios P. BogdanosFrontiers Media S.A.articlemultiple sclerosisdoxycyclineminocyclineproinflammatoryinterferon-γinterleukin-17Immunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	multiple sclerosis doxycycline minocycline proinflammatory interferon-γ interleukin-17 Immunologic diseases. Allergy RC581-607
spellingShingle	multiple sclerosis doxycycline minocycline proinflammatory interferon-γ interleukin-17 Immunologic diseases. Allergy RC581-607 Despoina T. Florou Despoina T. Florou Athanasios Mavropoulos Efthymios Dardiotis Vana Tsimourtou Vasileios Siokas Athina-Maria Aloizou Christos Liaskos Christina Tsigalou Christina Katsiari Lazaros I. Sakkas Georgios Hadjigeorgiou Georgios Hadjigeorgiou Dimitrios P. Bogdanos Tetracyclines Diminish In Vitro IFN-γ and IL-17-Producing Adaptive and Innate Immune Cells in Multiple Sclerosis
description	IntroductionLimited data from clinical trials in multiple sclerosis (MS) reported that minocycline, a widely used antibiotic belonging to the family of tetracyclines (TCs), exerts a beneficial short-lived clinical effect A similar anti-inflammatory effect of minocycline attributed to a deviation from Th1 to Th2 immune response has been reported in experimental models of MS. Whether such an immunomodulatory mechanism is operated in the human disease remains largely unknown.AimTo assess the in vitro immunomodulatory effect of tetracyclines, and in particular minocycline and doxycycline, in naïve and treated patients with MS.Material and MethodsPeripheral blood mononuclear cells from 45 individuals (35 MS patients, amongst which 15 naïve patients and 10 healthy controls, HCs) were cultured with minocycline or doxycycline and conventional stimulants (PMA/Ionomycin or IL-12/IL-18). IFN-γ and IL-17 producing T-, NK- and NKT cells were assessed by flow cytometry. The effect of TCs on cell viability and apoptosis was further assessed by flow cytometry with Annexin V staining.ResultsBoth tetracyclines significantly decreased, in a dose dependent manner, IFN-γ production in NKT and CD4+ T lymphocytes from MS patients (naïve or treated) stimulated with IL-12/IL-18 but did not decrease IFN-γ producing CD8+ T cells from naive MS or treated RRMS patients. They also decreased IL-17+ T and NKT cells following PMA and Ionomycin-stimulation. Tetracyclines did not affect the viability of cell subsets.ConclusionTetracyclines can in vitro suppress IFN-γ and IL-17- producing cells from MS patients, and this may explain their potential therapeutic effect in vivo.
format	article
author	Despoina T. Florou Despoina T. Florou Athanasios Mavropoulos Efthymios Dardiotis Vana Tsimourtou Vasileios Siokas Athina-Maria Aloizou Christos Liaskos Christina Tsigalou Christina Katsiari Lazaros I. Sakkas Georgios Hadjigeorgiou Georgios Hadjigeorgiou Dimitrios P. Bogdanos
author_facet	Despoina T. Florou Despoina T. Florou Athanasios Mavropoulos Efthymios Dardiotis Vana Tsimourtou Vasileios Siokas Athina-Maria Aloizou Christos Liaskos Christina Tsigalou Christina Katsiari Lazaros I. Sakkas Georgios Hadjigeorgiou Georgios Hadjigeorgiou Dimitrios P. Bogdanos
author_sort	Despoina T. Florou
title	Tetracyclines Diminish In Vitro IFN-γ and IL-17-Producing Adaptive and Innate Immune Cells in Multiple Sclerosis
title_short	Tetracyclines Diminish In Vitro IFN-γ and IL-17-Producing Adaptive and Innate Immune Cells in Multiple Sclerosis
title_full	Tetracyclines Diminish In Vitro IFN-γ and IL-17-Producing Adaptive and Innate Immune Cells in Multiple Sclerosis
title_fullStr	Tetracyclines Diminish In Vitro IFN-γ and IL-17-Producing Adaptive and Innate Immune Cells in Multiple Sclerosis
title_full_unstemmed	Tetracyclines Diminish In Vitro IFN-γ and IL-17-Producing Adaptive and Innate Immune Cells in Multiple Sclerosis
title_sort	tetracyclines diminish in vitro ifn-γ and il-17-producing adaptive and innate immune cells in multiple sclerosis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/ed1c5b548c154cf3805e7e12acf07aed
work_keys_str_mv	AT despoinatflorou tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT despoinatflorou tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT athanasiosmavropoulos tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT efthymiosdardiotis tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT vanatsimourtou tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT vasileiossiokas tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT athinamariaaloizou tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT christosliaskos tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT christinatsigalou tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT christinakatsiari tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT lazarosisakkas tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT georgioshadjigeorgiou tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT georgioshadjigeorgiou tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis AT dimitriospbogdanos tetracyclinesdiminishinvitroifngandil17producingadaptiveandinnateimmunecellsinmultiplesclerosis
_version_	1718405389103398912

Tetracyclines Diminish In Vitro IFN-γ and IL-17-Producing Adaptive and Innate Immune Cells in Multiple Sclerosis

Ejemplares similares