Anti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa

Background: Acanthamoeba is a globally dispersed protozoan that can cause different clinical manifestations in infected individuals. Various drugs have been proposed against its drug-resistant forms. Objective: The present study examined silver nanoparticles (NPs) with a good anti-parasitic backgro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Amir Karimipour-Saryazdi, Fatemeh Ghaffarifar, Pooya Tavakoli, Yeganeh Karimipour-Saryazdi, Leila Zaki, Saeed Bahadory
Formato:	article
Lenguaje:	EN
Publicado:	Alborz University of Medical Sciences 2020
Materias:	acanthamoeba silver nanoparticles anti-parasitic mtt flow cytometry Diseases of the digestive system. Gastroenterology RC799-869
Acceso en línea:	https://doaj.org/article/ed1e425078bb452f83bf8d176adabeb4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ed1e425078bb452f83bf8d176adabeb4
record_format	dspace
spelling	oai:doaj.org-article:ed1e425078bb452f83bf8d176adabeb42021-11-18T06:56:46ZAnti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa2345-33622322-586610.34172/ijep.2020.19https://doaj.org/article/ed1e425078bb452f83bf8d176adabeb42020-08-01T00:00:00Zhttp://enterpathog.abzums.ac.ir/PDF/ijep-4344https://doaj.org/toc/2345-3362https://doaj.org/toc/2322-5866Background: Acanthamoeba is a globally dispersed protozoan that can cause different clinical manifestations in infected individuals. Various drugs have been proposed against its drug-resistant forms. Objective: The present study examined silver nanoparticles (NPs) with a good anti-parasitic background. More precisely, the study focused on evaluating the anti-parasitic effect of silver nano-scale particles on protozoan trophozoite and cysts by microscopic counting and flow cytometry after exposure to different concentrations. Methods: To this end, MTT assay and IC50 were used to assess the macrophage toxicity and cysts/trophozoites, respectively. Results: Based on the results, 100 ppm silver NPs had better anti-parasitic effects than 80 ppm concentration and even the standard treatment of Acanthamoeba on both trophozoite and cystic phases. Macrophages toxicity at 100 ppm concentration was similar to the control group. Conclusion: In general, further studies should be conducted to confirm the present results given the significant effects of silver NPs against trophozoite and parasite cysts.Amir Karimipour-SaryazdiFatemeh GhaffarifarPooya TavakoliYeganeh Karimipour-SaryazdiLeila ZakiSaeed BahadoryAlborz University of Medical Sciencesarticleacanthamoeba silver nanoparticles anti-parasitic mtt flow cytometry Diseases of the digestive system. GastroenterologyRC799-869ENInternational Journal of Enteric Pathogens, Vol 8, Iss 3, Pp 84-88 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	acanthamoeba silver nanoparticles anti-parasitic mtt flow cytometry Diseases of the digestive system. Gastroenterology RC799-869
spellingShingle	acanthamoeba silver nanoparticles anti-parasitic mtt flow cytometry Diseases of the digestive system. Gastroenterology RC799-869 Amir Karimipour-Saryazdi Fatemeh Ghaffarifar Pooya Tavakoli Yeganeh Karimipour-Saryazdi Leila Zaki Saeed Bahadory Anti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa
description	Background: Acanthamoeba is a globally dispersed protozoan that can cause different clinical manifestations in infected individuals. Various drugs have been proposed against its drug-resistant forms. Objective: The present study examined silver nanoparticles (NPs) with a good anti-parasitic background. More precisely, the study focused on evaluating the anti-parasitic effect of silver nano-scale particles on protozoan trophozoite and cysts by microscopic counting and flow cytometry after exposure to different concentrations. Methods: To this end, MTT assay and IC50 were used to assess the macrophage toxicity and cysts/trophozoites, respectively. Results: Based on the results, 100 ppm silver NPs had better anti-parasitic effects than 80 ppm concentration and even the standard treatment of Acanthamoeba on both trophozoite and cystic phases. Macrophages toxicity at 100 ppm concentration was similar to the control group. Conclusion: In general, further studies should be conducted to confirm the present results given the significant effects of silver NPs against trophozoite and parasite cysts.
format	article
author	Amir Karimipour-Saryazdi Fatemeh Ghaffarifar Pooya Tavakoli Yeganeh Karimipour-Saryazdi Leila Zaki Saeed Bahadory
author_facet	Amir Karimipour-Saryazdi Fatemeh Ghaffarifar Pooya Tavakoli Yeganeh Karimipour-Saryazdi Leila Zaki Saeed Bahadory
author_sort	Amir Karimipour-Saryazdi
title	Anti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa
title_short	Anti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa
title_full	Anti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa
title_fullStr	Anti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa
title_full_unstemmed	Anti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa
title_sort	anti-parasitic effects of herbal extract-based silver nanoparticles on the trophozoite and cystic forms of acanthamoeba protozoa
publisher	Alborz University of Medical Sciences
publishDate	2020
url	https://doaj.org/article/ed1e425078bb452f83bf8d176adabeb4
work_keys_str_mv	AT amirkarimipoursaryazdi antiparasiticeffectsofherbalextractbasedsilvernanoparticlesonthetrophozoiteandcysticformsofacanthamoebaprotozoa AT fatemehghaffarifar antiparasiticeffectsofherbalextractbasedsilvernanoparticlesonthetrophozoiteandcysticformsofacanthamoebaprotozoa AT pooyatavakoli antiparasiticeffectsofherbalextractbasedsilvernanoparticlesonthetrophozoiteandcysticformsofacanthamoebaprotozoa AT yeganehkarimipoursaryazdi antiparasiticeffectsofherbalextractbasedsilvernanoparticlesonthetrophozoiteandcysticformsofacanthamoebaprotozoa AT leilazaki antiparasiticeffectsofherbalextractbasedsilvernanoparticlesonthetrophozoiteandcysticformsofacanthamoebaprotozoa AT saeedbahadory antiparasiticeffectsofherbalextractbasedsilvernanoparticlesonthetrophozoiteandcysticformsofacanthamoebaprotozoa
_version_	1718424203746607104

Anti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa

Ejemplares similares