Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria

Abstract. Apsari PP, Budiarti S, Wahyudi AT. 2019. Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria. Biodiversitas 20: 1259-1265. Based on the ability of actinomycetes as an antibacterial compounds producer and the need of finding novel ant...

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Autores principales:	PRAMESITA PRAWADIKA APSARI, SRI BUDIARTI, ARIS TRI WAHYUDI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	actinomycetes, antibacterials, gcms, urinary tract infection Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ed2292bd069c4459bf669a596a09d494
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spelling	oai:doaj.org-article:ed2292bd069c4459bf669a596a09d4942021-11-21T21:40:43ZActinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria1412-033X2085-472210.13057/biodiv/d200504https://doaj.org/article/ed2292bd069c4459bf669a596a09d4942019-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3178https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Apsari PP, Budiarti S, Wahyudi AT. 2019. Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria. Biodiversitas 20: 1259-1265. Based on the ability of actinomycetes as an antibacterial compounds producer and the need of finding novel antibacterial compounds, this study aims to look for antibacterial compounds from rhizosphere actinomycetes against bacteria in urine of UTI patients (Escherichia coli, Citrobacter braakii, Acinetobacter calcoaceticus, and Klebsiella pneumoniae). The screening of 21 actinomycetes was conducted based on the paper disc method. Potential actinomycetes that inhibited test bacteria were, then cultured in liquid medium and the supernatant was tested to six test bacteria. Then, the supernatant was extracted using ethyl acetate and crude extract from extraction process was tested to test bacteria. Afterward, the crude extract was scanned for bioactive compounds by GC-MS. Potential actinomycetes were identified by 16S rRNA gene to reveal the species. The screening results showed that ARJ 16, 24, and 36 had a wider inhibition zone than others. All of them showed that the supernatant and the crude extract could inhibit UTI's bacteria. The highest abundance of bioactive compound of crude extract was found in Propane, 1,2-dichloro, n-Hexadecanoic acid, and Carbonochloridic acid, 2-chloroethyl ester, respectively. Identification of potential actinomycetes based on 16S rRNA gene showed that ARJ 16 and ARJ 24 were highly similar to Streptomyces sp. in 99% and ARJ 36 was similar to Streptomyces tendae in 98%.PRAMESITA PRAWADIKA APSARISRI BUDIARTIARIS TRI WAHYUDIMBI & UNS Soloarticleactinomycetes, antibacterials, gcms, urinary tract infectionBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	actinomycetes, antibacterials, gcms, urinary tract infection Biology (General) QH301-705.5
spellingShingle	actinomycetes, antibacterials, gcms, urinary tract infection Biology (General) QH301-705.5 PRAMESITA PRAWADIKA APSARI SRI BUDIARTI ARIS TRI WAHYUDI Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria
description	Abstract. Apsari PP, Budiarti S, Wahyudi AT. 2019. Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria. Biodiversitas 20: 1259-1265. Based on the ability of actinomycetes as an antibacterial compounds producer and the need of finding novel antibacterial compounds, this study aims to look for antibacterial compounds from rhizosphere actinomycetes against bacteria in urine of UTI patients (Escherichia coli, Citrobacter braakii, Acinetobacter calcoaceticus, and Klebsiella pneumoniae). The screening of 21 actinomycetes was conducted based on the paper disc method. Potential actinomycetes that inhibited test bacteria were, then cultured in liquid medium and the supernatant was tested to six test bacteria. Then, the supernatant was extracted using ethyl acetate and crude extract from extraction process was tested to test bacteria. Afterward, the crude extract was scanned for bioactive compounds by GC-MS. Potential actinomycetes were identified by 16S rRNA gene to reveal the species. The screening results showed that ARJ 16, 24, and 36 had a wider inhibition zone than others. All of them showed that the supernatant and the crude extract could inhibit UTI's bacteria. The highest abundance of bioactive compound of crude extract was found in Propane, 1,2-dichloro, n-Hexadecanoic acid, and Carbonochloridic acid, 2-chloroethyl ester, respectively. Identification of potential actinomycetes based on 16S rRNA gene showed that ARJ 16 and ARJ 24 were highly similar to Streptomyces sp. in 99% and ARJ 36 was similar to Streptomyces tendae in 98%.
format	article
author	PRAMESITA PRAWADIKA APSARI SRI BUDIARTI ARIS TRI WAHYUDI
author_facet	PRAMESITA PRAWADIKA APSARI SRI BUDIARTI ARIS TRI WAHYUDI
author_sort	PRAMESITA PRAWADIKA APSARI
title	Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria
title_short	Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria
title_full	Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria
title_fullStr	Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria
title_full_unstemmed	Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria
title_sort	actinomycetes of rhizosphere soil producing antibacterial compounds against urinary tract infection bacteria
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/ed2292bd069c4459bf669a596a09d494
work_keys_str_mv	AT pramesitaprawadikaapsari actinomycetesofrhizospheresoilproducingantibacterialcompoundsagainsturinarytractinfectionbacteria AT sribudiarti actinomycetesofrhizospheresoilproducingantibacterialcompoundsagainsturinarytractinfectionbacteria AT aristriwahyudi actinomycetesofrhizospheresoilproducingantibacterialcompoundsagainsturinarytractinfectionbacteria
_version_	1718418677138718720

Actinomycetes of rhizosphere soil producing antibacterial compounds against Urinary Tract Infection bacteria

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