Manipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions.

Microalgae are a promising feedstock for renewable fuels, and algal metabolic engineering can lead to crop improvement, thus accelerating the development of commercially viable biodiesel production from algae biomass. We demonstrate that protein-protein interactions between the fatty acid acyl carri...

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Autores principales:	Jillian L Blatti, Joris Beld, Craig A Behnke, Michael Mendez, Stephen P Mayfield, Michael D Burkart
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed25096f3b4a4d7eacdeaf814c0baeb3
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spelling	oai:doaj.org-article:ed25096f3b4a4d7eacdeaf814c0baeb32021-11-18T07:05:45ZManipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions.1932-620310.1371/journal.pone.0042949https://doaj.org/article/ed25096f3b4a4d7eacdeaf814c0baeb32012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028438/?tool=EBIhttps://doaj.org/toc/1932-6203Microalgae are a promising feedstock for renewable fuels, and algal metabolic engineering can lead to crop improvement, thus accelerating the development of commercially viable biodiesel production from algae biomass. We demonstrate that protein-protein interactions between the fatty acid acyl carrier protein (ACP) and thioesterase (TE) govern fatty acid hydrolysis within the algal chloroplast. Using green microalga Chlamydomonas reinhardtii (Cr) as a model, a structural simulation of docking CrACP to CrTE identifies a protein-protein recognition surface between the two domains. A virtual screen reveals plant TEs with similar in silico binding to CrACP. Employing an activity-based crosslinking probe designed to selectively trap transient protein-protein interactions between the TE and ACP, we demonstrate in vitro that CrTE must functionally interact with CrACP to release fatty acids, while TEs of vascular plants show no mechanistic crosslinking to CrACP. This is recapitulated in vivo, where overproduction of the endogenous CrTE increased levels of short-chain fatty acids and engineering plant TEs into the C. reinhardtii chloroplast did not alter the fatty acid profile. These findings highlight the critical role of protein-protein interactions in manipulating fatty acid biosynthesis for algae biofuel engineering as illuminated by activity-based probes.Jillian L BlattiJoris BeldCraig A BehnkeMichael MendezStephen P MayfieldMichael D BurkartPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e42949 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jillian L Blatti Joris Beld Craig A Behnke Michael Mendez Stephen P Mayfield Michael D Burkart Manipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions.
description	Microalgae are a promising feedstock for renewable fuels, and algal metabolic engineering can lead to crop improvement, thus accelerating the development of commercially viable biodiesel production from algae biomass. We demonstrate that protein-protein interactions between the fatty acid acyl carrier protein (ACP) and thioesterase (TE) govern fatty acid hydrolysis within the algal chloroplast. Using green microalga Chlamydomonas reinhardtii (Cr) as a model, a structural simulation of docking CrACP to CrTE identifies a protein-protein recognition surface between the two domains. A virtual screen reveals plant TEs with similar in silico binding to CrACP. Employing an activity-based crosslinking probe designed to selectively trap transient protein-protein interactions between the TE and ACP, we demonstrate in vitro that CrTE must functionally interact with CrACP to release fatty acids, while TEs of vascular plants show no mechanistic crosslinking to CrACP. This is recapitulated in vivo, where overproduction of the endogenous CrTE increased levels of short-chain fatty acids and engineering plant TEs into the C. reinhardtii chloroplast did not alter the fatty acid profile. These findings highlight the critical role of protein-protein interactions in manipulating fatty acid biosynthesis for algae biofuel engineering as illuminated by activity-based probes.
format	article
author	Jillian L Blatti Joris Beld Craig A Behnke Michael Mendez Stephen P Mayfield Michael D Burkart
author_facet	Jillian L Blatti Joris Beld Craig A Behnke Michael Mendez Stephen P Mayfield Michael D Burkart
author_sort	Jillian L Blatti
title	Manipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions.
title_short	Manipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions.
title_full	Manipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions.
title_fullStr	Manipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions.
title_full_unstemmed	Manipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions.
title_sort	manipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ed25096f3b4a4d7eacdeaf814c0baeb3
work_keys_str_mv	AT jillianlblatti manipulatingfattyacidbiosynthesisinmicroalgaeforbiofuelthroughproteinproteininteractions AT jorisbeld manipulatingfattyacidbiosynthesisinmicroalgaeforbiofuelthroughproteinproteininteractions AT craigabehnke manipulatingfattyacidbiosynthesisinmicroalgaeforbiofuelthroughproteinproteininteractions AT michaelmendez manipulatingfattyacidbiosynthesisinmicroalgaeforbiofuelthroughproteinproteininteractions AT stephenpmayfield manipulatingfattyacidbiosynthesisinmicroalgaeforbiofuelthroughproteinproteininteractions AT michaeldburkart manipulatingfattyacidbiosynthesisinmicroalgaeforbiofuelthroughproteinproteininteractions
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Manipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions.

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