Cellular interaction of folic acid conjugated superparamagnetic iron oxide nanoparticles and its use as contrast agent for targeted magnetic imaging of tumor cells

Manoj Kumar,1 Gurpal Singh,1 Vikas Arora,2 Sujeet Mewar,3 Uma Sharma,3 NR Jagannathan,3 Sameer Sapra,2 Amit K Dinda,4 Surender Kharbanda,5 Harpal Singh11Center for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi-110016, India; 2Department of Chemistry, Indian Institute o...

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Autores principales:	Kumar M, Singh G, Arora V, Mewar S, Sharma U, Jagannathan NR, Sapra S, Dinda AK, Kharbanda S, Singh H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ed32453999f0418ab397e9f5a124ae01
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spelling	oai:doaj.org-article:ed32453999f0418ab397e9f5a124ae012021-12-02T08:37:12ZCellular interaction of folic acid conjugated superparamagnetic iron oxide nanoparticles and its use as contrast agent for targeted magnetic imaging of tumor cells1176-91141178-2013https://doaj.org/article/ed32453999f0418ab397e9f5a124ae012012-07-01T00:00:00Zhttp://www.dovepress.com/cellular-interaction-of-folic-acid-conjugated-superparamagnetic-iron-o-a10325https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Manoj Kumar,1 Gurpal Singh,1 Vikas Arora,2 Sujeet Mewar,3 Uma Sharma,3 NR Jagannathan,3 Sameer Sapra,2 Amit K Dinda,4 Surender Kharbanda,5 Harpal Singh11Center for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi-110016, India; 2Department of Chemistry, Indian Institute of Technology, Delhi Hauz Khas, New Delhi-110016, India; 3Department Of NMR, All India Institute of Medical Sciences, Ansari Nagar New Delhi-110029, India; 4Department Of Pathology, All India Institute of Medical Sciences, Ansari Nagar New Delhi-110029, India; 5Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USAAbstract: The purpose of the study was to develop tumor specific, water dispersible superparamagnetic iron oxide nanoparticles (SPIONs) and evaluate their efficacy as a contrast agent in magnetic resonance imaging (MRI). We have developed SPIONs capped with citric acid/2-bromo-2-methylpropionic acid which are compact, water dispersible, biocompatible having narrow range of size dispersity (8&ndash;10 nm), and relatively high T2 relaxivity (R2 = 222L &middot; mmol-1 &middot; sec-l). The targeting efficacy of unconjugated and folic acid-conjugated SPIONs (FA-SPIONS) was evaluated in a folic acid receptor overexpressing and negative tumor cell lines. Folic acid receptor-positive cells incubated with FA-SPIONs showed much higher intracellular iron content without any cytotoxicity. Ultrastructurally, SPIONs were seen as clustered inside the various stages of endocytic pathways without damaging cellular organelles and possible mechanism for their entry is via receptor mediated endocytosis. In vitro MRI studies on tumor cells showed better T2-weighted images in FA-SPIONs. These findings indicate that FA-SPIONs possess high colloidal stability with excellent sensitivity of imaging and can be a useful MRI contrast agent for the detection of cancer.Keywords: superparamagnetic iron oxide nanoparticles (SPION), cellular internalization, magnetic resonance imaging (MRI), cancer-cell targeting ligandKumar MSingh GArora VMewar SSharma UJagannathan NRSapra SDinda AKKharbanda SSingh HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3503-3516 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kumar M Singh G Arora V Mewar S Sharma U Jagannathan NR Sapra S Dinda AK Kharbanda S Singh H Cellular interaction of folic acid conjugated superparamagnetic iron oxide nanoparticles and its use as contrast agent for targeted magnetic imaging of tumor cells
description	Manoj Kumar,1 Gurpal Singh,1 Vikas Arora,2 Sujeet Mewar,3 Uma Sharma,3 NR Jagannathan,3 Sameer Sapra,2 Amit K Dinda,4 Surender Kharbanda,5 Harpal Singh11Center for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi-110016, India; 2Department of Chemistry, Indian Institute of Technology, Delhi Hauz Khas, New Delhi-110016, India; 3Department Of NMR, All India Institute of Medical Sciences, Ansari Nagar New Delhi-110029, India; 4Department Of Pathology, All India Institute of Medical Sciences, Ansari Nagar New Delhi-110029, India; 5Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USAAbstract: The purpose of the study was to develop tumor specific, water dispersible superparamagnetic iron oxide nanoparticles (SPIONs) and evaluate their efficacy as a contrast agent in magnetic resonance imaging (MRI). We have developed SPIONs capped with citric acid/2-bromo-2-methylpropionic acid which are compact, water dispersible, biocompatible having narrow range of size dispersity (8&ndash;10 nm), and relatively high T2 relaxivity (R2 = 222L &middot; mmol-1 &middot; sec-l). The targeting efficacy of unconjugated and folic acid-conjugated SPIONs (FA-SPIONS) was evaluated in a folic acid receptor overexpressing and negative tumor cell lines. Folic acid receptor-positive cells incubated with FA-SPIONs showed much higher intracellular iron content without any cytotoxicity. Ultrastructurally, SPIONs were seen as clustered inside the various stages of endocytic pathways without damaging cellular organelles and possible mechanism for their entry is via receptor mediated endocytosis. In vitro MRI studies on tumor cells showed better T2-weighted images in FA-SPIONs. These findings indicate that FA-SPIONs possess high colloidal stability with excellent sensitivity of imaging and can be a useful MRI contrast agent for the detection of cancer.Keywords: superparamagnetic iron oxide nanoparticles (SPION), cellular internalization, magnetic resonance imaging (MRI), cancer-cell targeting ligand
format	article
author	Kumar M Singh G Arora V Mewar S Sharma U Jagannathan NR Sapra S Dinda AK Kharbanda S Singh H
author_facet	Kumar M Singh G Arora V Mewar S Sharma U Jagannathan NR Sapra S Dinda AK Kharbanda S Singh H
author_sort	Kumar M
title	Cellular interaction of folic acid conjugated superparamagnetic iron oxide nanoparticles and its use as contrast agent for targeted magnetic imaging of tumor cells
title_short	Cellular interaction of folic acid conjugated superparamagnetic iron oxide nanoparticles and its use as contrast agent for targeted magnetic imaging of tumor cells
title_full	Cellular interaction of folic acid conjugated superparamagnetic iron oxide nanoparticles and its use as contrast agent for targeted magnetic imaging of tumor cells
title_fullStr	Cellular interaction of folic acid conjugated superparamagnetic iron oxide nanoparticles and its use as contrast agent for targeted magnetic imaging of tumor cells
title_full_unstemmed	Cellular interaction of folic acid conjugated superparamagnetic iron oxide nanoparticles and its use as contrast agent for targeted magnetic imaging of tumor cells
title_sort	cellular interaction of folic acid conjugated superparamagnetic iron oxide nanoparticles and its use as contrast agent for targeted magnetic imaging of tumor cells
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/ed32453999f0418ab397e9f5a124ae01
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Cellular interaction of folic acid conjugated superparamagnetic iron oxide nanoparticles and its use as contrast agent for targeted magnetic imaging of tumor cells

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