Alterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes

Adipogenesis regulation is crucial for mature adipocyte function. In obesity, a major driver of type 2 diabetes (T2D), this process is disrupted and remains poorly characterized. Here we identified altered DNA methylation profiles in diabetic obese patients, during three adipocytes differentiation s...

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Autores principales:	Elaheh Mirzaeicheshmeh, Carlos Zerrweck, Federico Centeno-Cruz, Paulina Baca-Peynado, Angélica Martinez-Hernandez, Humberto García-Ortiz, Cecilia Contreras-Cubas, María Guadalupe Salas-Martínez, Yolanda Saldaña-Alvarez, Elvia C. Mendoza-Caamal, Francisco Barajas-Olmos, Lorena Orozco
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	adipogenesis type 2 diabetes obesity dna methylation Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/ed378195d09546b59a8bcf02ff88a8be
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spelling	oai:doaj.org-article:ed378195d09546b59a8bcf02ff88a8be2021-11-04T15:00:45ZAlterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes2162-39452162-397X10.1080/21623945.2021.1978157https://doaj.org/article/ed378195d09546b59a8bcf02ff88a8be2021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21623945.2021.1978157https://doaj.org/toc/2162-3945https://doaj.org/toc/2162-397XAdipogenesis regulation is crucial for mature adipocyte function. In obesity, a major driver of type 2 diabetes (T2D), this process is disrupted and remains poorly characterized. Here we identified altered DNA methylation profiles in diabetic obese patients, during three adipocytes differentiation stages. We isolated mesenchymal cells from visceral adipose tissue of obese patients with and without T2D to analyse DNA methylation profiles at 0, 3, and 18 days of ex vivo differentiation and documented their impact on gene expression. Methylation and gene expression were analysed with EPIC and Clarion S arrays, respectively. Patients with T2D had epigenetic alterations in all the analysed stages, and these were mainly observed in genes important in adipogenesis, insulin resistance, cell death programming, and immune effector processes. Importantly, at 3 days, we found six-fold more methylated CpG alterations than in the other stages. This is the first study to document epigenetic markers that persist through all three adipogenesis stages and their impact on gene expression, which could be a cellular metabolic memory involved in T2D. Our data provided evidence that, throughout the adipogenesis process, alterations occur in methylation that might impact mature adipocyte function, cause tissue malfunction, and potentially, lead to the development of T2D.Elaheh MirzaeicheshmehCarlos ZerrweckFederico Centeno-CruzPaulina Baca-PeynadoAngélica Martinez-HernandezHumberto García-OrtizCecilia Contreras-CubasMaría Guadalupe Salas-MartínezYolanda Saldaña-AlvarezElvia C. Mendoza-CaamalFrancisco Barajas-OlmosLorena OrozcoTaylor & Francis Grouparticleadipogenesistype 2 diabetesobesitydna methylationDiseases of the endocrine glands. Clinical endocrinologyRC648-665CytologyQH573-671PhysiologyQP1-981ENAdipocyte, Vol 10, Iss 1, Pp 493-504 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipogenesis type 2 diabetes obesity dna methylation Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
spellingShingle	adipogenesis type 2 diabetes obesity dna methylation Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981 Elaheh Mirzaeicheshmeh Carlos Zerrweck Federico Centeno-Cruz Paulina Baca-Peynado Angélica Martinez-Hernandez Humberto García-Ortiz Cecilia Contreras-Cubas María Guadalupe Salas-Martínez Yolanda Saldaña-Alvarez Elvia C. Mendoza-Caamal Francisco Barajas-Olmos Lorena Orozco Alterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes
description	Adipogenesis regulation is crucial for mature adipocyte function. In obesity, a major driver of type 2 diabetes (T2D), this process is disrupted and remains poorly characterized. Here we identified altered DNA methylation profiles in diabetic obese patients, during three adipocytes differentiation stages. We isolated mesenchymal cells from visceral adipose tissue of obese patients with and without T2D to analyse DNA methylation profiles at 0, 3, and 18 days of ex vivo differentiation and documented their impact on gene expression. Methylation and gene expression were analysed with EPIC and Clarion S arrays, respectively. Patients with T2D had epigenetic alterations in all the analysed stages, and these were mainly observed in genes important in adipogenesis, insulin resistance, cell death programming, and immune effector processes. Importantly, at 3 days, we found six-fold more methylated CpG alterations than in the other stages. This is the first study to document epigenetic markers that persist through all three adipogenesis stages and their impact on gene expression, which could be a cellular metabolic memory involved in T2D. Our data provided evidence that, throughout the adipogenesis process, alterations occur in methylation that might impact mature adipocyte function, cause tissue malfunction, and potentially, lead to the development of T2D.
format	article
author	Elaheh Mirzaeicheshmeh Carlos Zerrweck Federico Centeno-Cruz Paulina Baca-Peynado Angélica Martinez-Hernandez Humberto García-Ortiz Cecilia Contreras-Cubas María Guadalupe Salas-Martínez Yolanda Saldaña-Alvarez Elvia C. Mendoza-Caamal Francisco Barajas-Olmos Lorena Orozco
author_facet	Elaheh Mirzaeicheshmeh Carlos Zerrweck Federico Centeno-Cruz Paulina Baca-Peynado Angélica Martinez-Hernandez Humberto García-Ortiz Cecilia Contreras-Cubas María Guadalupe Salas-Martínez Yolanda Saldaña-Alvarez Elvia C. Mendoza-Caamal Francisco Barajas-Olmos Lorena Orozco
author_sort	Elaheh Mirzaeicheshmeh
title	Alterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes
title_short	Alterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes
title_full	Alterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes
title_fullStr	Alterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes
title_full_unstemmed	Alterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes
title_sort	alterations of dna methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/ed378195d09546b59a8bcf02ff88a8be
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Alterations of DNA methylation during adipogenesis differentiation of mesenchymal stem cells isolated from adipose tissue of patients with obesity is associated with type 2 diabetes

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