Combined Therapy with microRNA-Expressing <i>Salmonella</i> and Irradiation in Melanoma

Anticancer treatment strategies using bacteria as a vector are currently expanding with the development of anticancer drugs. Here, we present a research strategy to develop anticancer drugs using bacteria that contain miRNAs. We also present a strategy for the development of novel bacterial anticanc...

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Autores principales:	Wonsuck Yoon, Yongsung Park, Seunghyun Kim, Yongkeun Park, Chul Yong Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	melanoma bacterial treatment <i>Salmonella</i> <i>Typhimurium</i> radiation therapy Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ed3b0de0d90c479a9950630de53caa3c
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spelling	oai:doaj.org-article:ed3b0de0d90c479a9950630de53caa3c2021-11-25T18:25:46ZCombined Therapy with microRNA-Expressing <i>Salmonella</i> and Irradiation in Melanoma10.3390/microorganisms91124082076-2607https://doaj.org/article/ed3b0de0d90c479a9950630de53caa3c2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2408https://doaj.org/toc/2076-2607Anticancer treatment strategies using bacteria as a vector are currently expanding with the development of anticancer drugs. Here, we present a research strategy to develop anticancer drugs using bacteria that contain miRNAs. We also present a strategy for the development of novel bacterial anticancer drugs in combination with radiation. <i>Salmonella</i> strains expressing miRNA were produced by modifying the miRNA expression vector encoding INHA, a radiation-resistant gene developed previously. The anticancer effect of INHA was confirmed using skin cancer cell lines. We also tested a combination strategy comprising bacteria and radiation for its anticancer efficacy against radiation-resistant mouse melanoma to increase the efficacy of radiation therapy as a novel strategy. The recombinant strain was confirmed to promote effective cell death even when combined with radiation therapy, which exerts its cytotoxicity by enhancing reactive oxygen species production. Moreover, a combination of bacterial and radiation therapy enhanced radiotherapy efficacy. When combined with radiation therapy, bacterial therapy exhibited effective anti-cancer properties even when administered to animals harboring radiation-resistant tumors. This strategy may promote the secretion of cytokines in cells and more effectively reduce the number of bacteria remaining in the animal. Thus, this study may lead to the development of a strategy to improve the effectiveness of radiation therapy using <i>Salmonella</i> expressing cancer-specific miRNA for intractable cancers such as those resistant to radiation.Wonsuck YoonYongsung ParkSeunghyun KimYongkeun ParkChul Yong KimMDPI AGarticlemelanomabacterial treatment<i>Salmonella</i> <i>Typhimurium</i>radiation therapyBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2408, p 2408 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	melanoma bacterial treatment <i>Salmonella</i> <i>Typhimurium</i> radiation therapy Biology (General) QH301-705.5
spellingShingle	melanoma bacterial treatment <i>Salmonella</i> <i>Typhimurium</i> radiation therapy Biology (General) QH301-705.5 Wonsuck Yoon Yongsung Park Seunghyun Kim Yongkeun Park Chul Yong Kim Combined Therapy with microRNA-Expressing <i>Salmonella</i> and Irradiation in Melanoma
description	Anticancer treatment strategies using bacteria as a vector are currently expanding with the development of anticancer drugs. Here, we present a research strategy to develop anticancer drugs using bacteria that contain miRNAs. We also present a strategy for the development of novel bacterial anticancer drugs in combination with radiation. <i>Salmonella</i> strains expressing miRNA were produced by modifying the miRNA expression vector encoding INHA, a radiation-resistant gene developed previously. The anticancer effect of INHA was confirmed using skin cancer cell lines. We also tested a combination strategy comprising bacteria and radiation for its anticancer efficacy against radiation-resistant mouse melanoma to increase the efficacy of radiation therapy as a novel strategy. The recombinant strain was confirmed to promote effective cell death even when combined with radiation therapy, which exerts its cytotoxicity by enhancing reactive oxygen species production. Moreover, a combination of bacterial and radiation therapy enhanced radiotherapy efficacy. When combined with radiation therapy, bacterial therapy exhibited effective anti-cancer properties even when administered to animals harboring radiation-resistant tumors. This strategy may promote the secretion of cytokines in cells and more effectively reduce the number of bacteria remaining in the animal. Thus, this study may lead to the development of a strategy to improve the effectiveness of radiation therapy using <i>Salmonella</i> expressing cancer-specific miRNA for intractable cancers such as those resistant to radiation.
format	article
author	Wonsuck Yoon Yongsung Park Seunghyun Kim Yongkeun Park Chul Yong Kim
author_facet	Wonsuck Yoon Yongsung Park Seunghyun Kim Yongkeun Park Chul Yong Kim
author_sort	Wonsuck Yoon
title	Combined Therapy with microRNA-Expressing <i>Salmonella</i> and Irradiation in Melanoma
title_short	Combined Therapy with microRNA-Expressing <i>Salmonella</i> and Irradiation in Melanoma
title_full	Combined Therapy with microRNA-Expressing <i>Salmonella</i> and Irradiation in Melanoma
title_fullStr	Combined Therapy with microRNA-Expressing <i>Salmonella</i> and Irradiation in Melanoma
title_full_unstemmed	Combined Therapy with microRNA-Expressing <i>Salmonella</i> and Irradiation in Melanoma
title_sort	combined therapy with microrna-expressing <i>salmonella</i> and irradiation in melanoma
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ed3b0de0d90c479a9950630de53caa3c
work_keys_str_mv	AT wonsuckyoon combinedtherapywithmicrornaexpressingisalmonellaiandirradiationinmelanoma AT yongsungpark combinedtherapywithmicrornaexpressingisalmonellaiandirradiationinmelanoma AT seunghyunkim combinedtherapywithmicrornaexpressingisalmonellaiandirradiationinmelanoma AT yongkeunpark combinedtherapywithmicrornaexpressingisalmonellaiandirradiationinmelanoma AT chulyongkim combinedtherapywithmicrornaexpressingisalmonellaiandirradiationinmelanoma
_version_	1718411168497795072

Combined Therapy with microRNA-Expressing <i>Salmonella</i> and Irradiation in Melanoma

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