Remote ischemic preconditioning (RIPC) modifies plasma proteome in humans.

Remote Ischemic Preconditioning (RIPC) induced by brief episodes of ischemia of the limb protects against multi-organ damage by ischemia-reperfusion (IR). Although it has been demonstrated that RIPC affects gene expression, the proteomic response to RIPC has not been determined. This study aimed to...

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Autores principales:	Michele Hepponstall, Vera Ignjatovic, Steve Binos, Paul Monagle, Bryn Jones, Michael H H Cheung, Yves d'Udekem, Igor E Konstantinov
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed3f6752fd1f475e8a73de915b32030b
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spelling	oai:doaj.org-article:ed3f6752fd1f475e8a73de915b32030b2021-11-18T08:10:06ZRemote ischemic preconditioning (RIPC) modifies plasma proteome in humans.1932-620310.1371/journal.pone.0048284https://doaj.org/article/ed3f6752fd1f475e8a73de915b32030b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23139772/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Remote Ischemic Preconditioning (RIPC) induced by brief episodes of ischemia of the limb protects against multi-organ damage by ischemia-reperfusion (IR). Although it has been demonstrated that RIPC affects gene expression, the proteomic response to RIPC has not been determined. This study aimed to examine RIPC induced changes in the plasma proteome. Five healthy adult volunteers had 4 cycles of 5 min ischemia alternating with 5 min reperfusion of the forearm. Blood samples were taken from the ipsilateral arm prior to first ischaemia, immediately after each episode of ischemia as well as, at 15 min and 24 h after the last episode of ischemia. Plasma samples from five individuals were analysed using two complementary techniques. Individual samples were analysed using 2Dimensional Difference in gel electrophoresis (2D DIGE) and mass spectrometry (MS). Pooled samples for each of the time-points underwent trypsin digestion and peptides generated were analysed in triplicate using Liquid Chromatography and MS (LC-MS). Six proteins changed in response to RIPC using 2D DIGE analysis, while 48 proteins were found to be differentially regulated using LC-MS. The proteins of interest were involved in acute phase response signalling, and physiological molecular and cellular functions. The RIPC stimulus modifies the plasma protein content in blood taken from the ischemic arm in a cumulative fashion and evokes a proteomic response in peripheral blood.Michele HepponstallVera IgnjatovicSteve BinosPaul MonagleBryn JonesMichael H H CheungYves d'UdekemIgor E KonstantinovPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48284 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michele Hepponstall Vera Ignjatovic Steve Binos Paul Monagle Bryn Jones Michael H H Cheung Yves d'Udekem Igor E Konstantinov Remote ischemic preconditioning (RIPC) modifies plasma proteome in humans.
description	Remote Ischemic Preconditioning (RIPC) induced by brief episodes of ischemia of the limb protects against multi-organ damage by ischemia-reperfusion (IR). Although it has been demonstrated that RIPC affects gene expression, the proteomic response to RIPC has not been determined. This study aimed to examine RIPC induced changes in the plasma proteome. Five healthy adult volunteers had 4 cycles of 5 min ischemia alternating with 5 min reperfusion of the forearm. Blood samples were taken from the ipsilateral arm prior to first ischaemia, immediately after each episode of ischemia as well as, at 15 min and 24 h after the last episode of ischemia. Plasma samples from five individuals were analysed using two complementary techniques. Individual samples were analysed using 2Dimensional Difference in gel electrophoresis (2D DIGE) and mass spectrometry (MS). Pooled samples for each of the time-points underwent trypsin digestion and peptides generated were analysed in triplicate using Liquid Chromatography and MS (LC-MS). Six proteins changed in response to RIPC using 2D DIGE analysis, while 48 proteins were found to be differentially regulated using LC-MS. The proteins of interest were involved in acute phase response signalling, and physiological molecular and cellular functions. The RIPC stimulus modifies the plasma protein content in blood taken from the ischemic arm in a cumulative fashion and evokes a proteomic response in peripheral blood.
format	article
author	Michele Hepponstall Vera Ignjatovic Steve Binos Paul Monagle Bryn Jones Michael H H Cheung Yves d'Udekem Igor E Konstantinov
author_facet	Michele Hepponstall Vera Ignjatovic Steve Binos Paul Monagle Bryn Jones Michael H H Cheung Yves d'Udekem Igor E Konstantinov
author_sort	Michele Hepponstall
title	Remote ischemic preconditioning (RIPC) modifies plasma proteome in humans.
title_short	Remote ischemic preconditioning (RIPC) modifies plasma proteome in humans.
title_full	Remote ischemic preconditioning (RIPC) modifies plasma proteome in humans.
title_fullStr	Remote ischemic preconditioning (RIPC) modifies plasma proteome in humans.
title_full_unstemmed	Remote ischemic preconditioning (RIPC) modifies plasma proteome in humans.
title_sort	remote ischemic preconditioning (ripc) modifies plasma proteome in humans.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ed3f6752fd1f475e8a73de915b32030b
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Remote ischemic preconditioning (RIPC) modifies plasma proteome in humans.

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