Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress

Abstract Dehydrins belong to the group 2 family LEA (Late Embryogenesis Abundant) proteins, which are up-regulated in most plants during cold and drought stress. According to the number and order of the Y-, S- and K-segments, dehydrins are classified into five subclasses: YnSKn, YnKn, SKn, Kn and Kn...

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Autores principales:	Yang Liu, Li Wang, Tianpeng Zhang, Xinghong Yang, Dequan Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed470b0c0e9044369edac78c8f8e234d
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spelling	oai:doaj.org-article:ed470b0c0e9044369edac78c8f8e234d2021-12-02T16:06:49ZFunctional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress10.1038/s41598-017-07852-y2045-2322https://doaj.org/article/ed470b0c0e9044369edac78c8f8e234d2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07852-yhttps://doaj.org/toc/2045-2322Abstract Dehydrins belong to the group 2 family LEA (Late Embryogenesis Abundant) proteins, which are up-regulated in most plants during cold and drought stress. According to the number and order of the Y-, S- and K-segments, dehydrins are classified into five subclasses: YnSKn, YnKn, SKn, Kn and KnS. Here, the maize (Zea mays L.) KS-type dehydrin gene, ZmDHN13, was identified and later characterized. Expression profiling demonstrated that ZmDHN13 was constitutively expressed, but its expression was also altered by high osmosis, low temperature, oxidative stress and abscisic acid (ABA). Furthermore, the roles of the three conserved segments in phosphorylation, localization, binding metal ions and physiological functions were explored. ZmDHN13 was mainly localized in the nucleus, depending on phosphorylation status. Additional studies indicated that ZmDHN13 could be phosphorylated by CKII (casein kinase II), when the NLS (nuclear localization signal) segment and the S-segment were core sequences. The overexpression of ZmDHN13 enhanced transgenic tobacco tolerance to oxidative stress, and the three conserved segments exhibited a cooperative effect in response to environmental stresses in vivo.Yang LiuLi WangTianpeng ZhangXinghong YangDequan LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Liu Li Wang Tianpeng Zhang Xinghong Yang Dequan Li Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress
description	Abstract Dehydrins belong to the group 2 family LEA (Late Embryogenesis Abundant) proteins, which are up-regulated in most plants during cold and drought stress. According to the number and order of the Y-, S- and K-segments, dehydrins are classified into five subclasses: YnSKn, YnKn, SKn, Kn and KnS. Here, the maize (Zea mays L.) KS-type dehydrin gene, ZmDHN13, was identified and later characterized. Expression profiling demonstrated that ZmDHN13 was constitutively expressed, but its expression was also altered by high osmosis, low temperature, oxidative stress and abscisic acid (ABA). Furthermore, the roles of the three conserved segments in phosphorylation, localization, binding metal ions and physiological functions were explored. ZmDHN13 was mainly localized in the nucleus, depending on phosphorylation status. Additional studies indicated that ZmDHN13 could be phosphorylated by CKII (casein kinase II), when the NLS (nuclear localization signal) segment and the S-segment were core sequences. The overexpression of ZmDHN13 enhanced transgenic tobacco tolerance to oxidative stress, and the three conserved segments exhibited a cooperative effect in response to environmental stresses in vivo.
format	article
author	Yang Liu Li Wang Tianpeng Zhang Xinghong Yang Dequan Li
author_facet	Yang Liu Li Wang Tianpeng Zhang Xinghong Yang Dequan Li
author_sort	Yang Liu
title	Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress
title_short	Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress
title_full	Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress
title_fullStr	Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress
title_full_unstemmed	Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress
title_sort	functional characterization of ks-type dehydrin zmdhn13 and its related conserved domains under oxidative stress
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ed470b0c0e9044369edac78c8f8e234d
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_version_	1718384876603834368

Functional characterization of KS-type dehydrin ZmDHN13 and its related conserved domains under oxidative stress

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