Circular RNA UBR1 promotes the proliferation, migration and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis

Lung cancer (LC) is a malignant tumor with the highest incidence in the world, and its specific pathogenesis is still unclear. Circular RNAs (circRNAs) are a group of non-coding RNAs that play a key role in the development and progression of various cancers. The expression pattern and function of ci...

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Autores principales:	Peng Su, Feng Mao, Jian Zhang, Hui Zhang, MingBo Wang, YanZhao Xu, ZiQiang Tian
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	lung cancer circular rna ubr1 microrna-545-5p ssfa2 proliferation migration invasion apoptosis Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/ed4fb673b5504bc9a79ad17d910caab1
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spelling	oai:doaj.org-article:ed4fb673b5504bc9a79ad17d910caab12021-11-26T11:19:49ZCircular RNA UBR1 promotes the proliferation, migration and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis2165-59792165-598710.1080/21655979.2021.2004977https://doaj.org/article/ed4fb673b5504bc9a79ad17d910caab12021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2004977https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Lung cancer (LC) is a malignant tumor with the highest incidence in the world, and its specific pathogenesis is still unclear. Circular RNAs (circRNAs) are a group of non-coding RNAs that play a key role in the development and progression of various cancers. The expression pattern and function of circRNAs in LC are still not completely distinct. In this study, it was aimed to study the expression and potential mechanism of circ-UBR1 in LC cells. Then it was found that circ-UBR1 was up-regulated in LC cells, and had microRNA (miR)-545-5p binding sites. Meanwhile, it was confirmed by dual luciferase reporter assay that, circ-UBR1 directly bound to miR-545-5p and then repressed its expression. MiR-545-5p was down-regulated in LC cells and refrained its expression by binding to the downstream target gene SSFA2. Knockdown circ-UBR1 or enhancive miR-545-5p repressed A549 cell proliferation, migration and invasion, but accelerated apoptosis. After transfection with circ-UBR1 low expression vector, upregulation of SSFA2 apparently reversed the depression of reduced circ-UBR1 on cell proliferation, migration and invasion, and the promotion of cell apoptosis. Further tumor xenograft experiments in nude mice also confirmed that knockdown of circ-UBR1 could increase the expression of miR-545-5p, but decrease the expression of SSFA2, thus alleviating the progression of LC in vivo. Therefore, these results fully indicates that circ-UBR1 promotes LC cell proliferation, migration and invasion, but represses apoptosis via the circ-UBR1 axis, which may be a closely related marker and therapeutic target of LC.Peng SuFeng MaoJian ZhangHui ZhangMingBo WangYanZhao XuZiQiang TianTaylor & Francis Grouparticlelung cancercircular rna ubr1microrna-545-5pssfa2proliferationmigrationinvasionapoptosisBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lung cancer circular rna ubr1 microrna-545-5p ssfa2 proliferation migration invasion apoptosis Biotechnology TP248.13-248.65
spellingShingle	lung cancer circular rna ubr1 microrna-545-5p ssfa2 proliferation migration invasion apoptosis Biotechnology TP248.13-248.65 Peng Su Feng Mao Jian Zhang Hui Zhang MingBo Wang YanZhao Xu ZiQiang Tian Circular RNA UBR1 promotes the proliferation, migration and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis
description	Lung cancer (LC) is a malignant tumor with the highest incidence in the world, and its specific pathogenesis is still unclear. Circular RNAs (circRNAs) are a group of non-coding RNAs that play a key role in the development and progression of various cancers. The expression pattern and function of circRNAs in LC are still not completely distinct. In this study, it was aimed to study the expression and potential mechanism of circ-UBR1 in LC cells. Then it was found that circ-UBR1 was up-regulated in LC cells, and had microRNA (miR)-545-5p binding sites. Meanwhile, it was confirmed by dual luciferase reporter assay that, circ-UBR1 directly bound to miR-545-5p and then repressed its expression. MiR-545-5p was down-regulated in LC cells and refrained its expression by binding to the downstream target gene SSFA2. Knockdown circ-UBR1 or enhancive miR-545-5p repressed A549 cell proliferation, migration and invasion, but accelerated apoptosis. After transfection with circ-UBR1 low expression vector, upregulation of SSFA2 apparently reversed the depression of reduced circ-UBR1 on cell proliferation, migration and invasion, and the promotion of cell apoptosis. Further tumor xenograft experiments in nude mice also confirmed that knockdown of circ-UBR1 could increase the expression of miR-545-5p, but decrease the expression of SSFA2, thus alleviating the progression of LC in vivo. Therefore, these results fully indicates that circ-UBR1 promotes LC cell proliferation, migration and invasion, but represses apoptosis via the circ-UBR1 axis, which may be a closely related marker and therapeutic target of LC.
format	article
author	Peng Su Feng Mao Jian Zhang Hui Zhang MingBo Wang YanZhao Xu ZiQiang Tian
author_facet	Peng Su Feng Mao Jian Zhang Hui Zhang MingBo Wang YanZhao Xu ZiQiang Tian
author_sort	Peng Su
title	Circular RNA UBR1 promotes the proliferation, migration and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis
title_short	Circular RNA UBR1 promotes the proliferation, migration and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis
title_full	Circular RNA UBR1 promotes the proliferation, migration and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis
title_fullStr	Circular RNA UBR1 promotes the proliferation, migration and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis
title_full_unstemmed	Circular RNA UBR1 promotes the proliferation, migration and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis
title_sort	circular rna ubr1 promotes the proliferation, migration and invasion but represses apoptosis of lung cancer cells via modulating microrna-545-5p/ssfa2 axis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/ed4fb673b5504bc9a79ad17d910caab1
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Circular RNA UBR1 promotes the proliferation, migration and invasion but represses apoptosis of lung cancer cells via modulating microRNA-545-5p/SSFA2 axis

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