Combining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology

Background: Within the last decade, confocal microscopy has become a valuable non-invasive diagnostic tool in imaging human skin in vivo. Of the two different methods that exist, reflectance confocal microscopy (RCM) displays the backscattering signal of naturally occurring skin components, whereas...

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Autores principales:	Hans Skvara, Ulrike Plut, Johannes A. Schmid, Constanze Jonak
Formato:	article
Lenguaje:	EN
Publicado:	Mattioli1885 2012
Materias:	confocal microscopy fluorescence reflectance sodium fluorescein in vivo healthy human skin Dermatology RL1-803
Acceso en línea:	https://doaj.org/article/ed5bde5a7ede4a6bb8cd0c71f9026779
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spelling	oai:doaj.org-article:ed5bde5a7ede4a6bb8cd0c71f90267792021-11-17T08:33:18ZCombining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology10.5826/dpc.0201a022160-9381https://doaj.org/article/ed5bde5a7ede4a6bb8cd0c71f90267792012-01-01T00:00:00Zhttp://dpcj.org/index.php/dpc/article/view/994https://doaj.org/toc/2160-9381 Background: Within the last decade, confocal microscopy has become a valuable non-invasive diagnostic tool in imaging human skin in vivo. Of the two different methods that exist, reflectance confocal microscopy (RCM) displays the backscattering signal of naturally occurring skin components, whereas fluorescence confocal microscopy (FCM) provides contrast by using an exogenously applied fluorescent dye. Methodology: A newly developed multilaser device, in which both techniques are implemented, has been used to combine both methods and allows to highlight different information in one image. In our study, we applied the fluorophore sodium fluorescein (SFL) intradermally on forearm skin of 10 healthy volunteers followed by fluorescence and reflectance imaging. Results: In fluorescence mode the intercellular distribution of SFL clearly outlines every single cell in the epidermis, whereas in reflectance mode keratin and melanin-rich cells and structures provide additional information. The combination of both methods enables a clear delineation between the cell border, the cytoplasm and the nucleus. Imaging immediately, 20, 40 and 60 minutes after SFL injection, represents the dynamic distribution pattern of the dye. Conclusion: The synergism of RCM and FCM in one device delivering accurate information on skin architecture and pigmentation will have a great impact on in vivo diagnosis of human skin in the future. Hans SkvaraUlrike PlutJohannes A. SchmidConstanze JonakMattioli1885articleconfocal microscopyfluorescencereflectancesodium fluoresceinin vivohealthy human skinDermatologyRL1-803ENDermatology Practical & Conceptual (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	confocal microscopy fluorescence reflectance sodium fluorescein in vivo healthy human skin Dermatology RL1-803
spellingShingle	confocal microscopy fluorescence reflectance sodium fluorescein in vivo healthy human skin Dermatology RL1-803 Hans Skvara Ulrike Plut Johannes A. Schmid Constanze Jonak Combining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology
description	Background: Within the last decade, confocal microscopy has become a valuable non-invasive diagnostic tool in imaging human skin in vivo. Of the two different methods that exist, reflectance confocal microscopy (RCM) displays the backscattering signal of naturally occurring skin components, whereas fluorescence confocal microscopy (FCM) provides contrast by using an exogenously applied fluorescent dye. Methodology: A newly developed multilaser device, in which both techniques are implemented, has been used to combine both methods and allows to highlight different information in one image. In our study, we applied the fluorophore sodium fluorescein (SFL) intradermally on forearm skin of 10 healthy volunteers followed by fluorescence and reflectance imaging. Results: In fluorescence mode the intercellular distribution of SFL clearly outlines every single cell in the epidermis, whereas in reflectance mode keratin and melanin-rich cells and structures provide additional information. The combination of both methods enables a clear delineation between the cell border, the cytoplasm and the nucleus. Imaging immediately, 20, 40 and 60 minutes after SFL injection, represents the dynamic distribution pattern of the dye. Conclusion: The synergism of RCM and FCM in one device delivering accurate information on skin architecture and pigmentation will have a great impact on in vivo diagnosis of human skin in the future.
format	article
author	Hans Skvara Ulrike Plut Johannes A. Schmid Constanze Jonak
author_facet	Hans Skvara Ulrike Plut Johannes A. Schmid Constanze Jonak
author_sort	Hans Skvara
title	Combining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology
title_short	Combining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology
title_full	Combining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology
title_fullStr	Combining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology
title_full_unstemmed	Combining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology
title_sort	combining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology
publisher	Mattioli1885
publishDate	2012
url	https://doaj.org/article/ed5bde5a7ede4a6bb8cd0c71f9026779
work_keys_str_mv	AT hansskvara combininginvivoreflectancewithfluorescenceconfocalmicroscopyprovidesadditiveinformationonskinmorphology AT ulrikeplut combininginvivoreflectancewithfluorescenceconfocalmicroscopyprovidesadditiveinformationonskinmorphology AT johannesaschmid combininginvivoreflectancewithfluorescenceconfocalmicroscopyprovidesadditiveinformationonskinmorphology AT constanzejonak combininginvivoreflectancewithfluorescenceconfocalmicroscopyprovidesadditiveinformationonskinmorphology
_version_	1718425707838701568

Combining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology

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