Genetic relationship between cocirculating Human enteroviruses species C.

Recombination events between human enteroviruses (HEV) are known to occur frequently and to participate in the evolution of these viruses. In a previous study, we reported the isolation of a panel of viruses belonging to the Human enterovirus species C (HEV-C) that had been cocirculating in a small...

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Autores principales:	Maël Bessaud, Marie-Line Joffret, Barbara Holmblat, Richter Razafindratsimandresy, Francis Delpeyroux
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed5c29fae7604bbc836c8f8163c5cb3a
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spelling	oai:doaj.org-article:ed5c29fae7604bbc836c8f8163c5cb3a2021-11-04T06:08:47ZGenetic relationship between cocirculating Human enteroviruses species C.1932-620310.1371/journal.pone.0024823https://doaj.org/article/ed5c29fae7604bbc836c8f8163c5cb3a2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21931857/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Recombination events between human enteroviruses (HEV) are known to occur frequently and to participate in the evolution of these viruses. In a previous study, we reported the isolation of a panel of viruses belonging to the Human enterovirus species C (HEV-C) that had been cocirculating in a small geographic area of Madagascar in 2002. This panel included type 2 vaccine-derived polioviruses (PV) that had caused several cases of acute flaccid paralysis in humans. Previous partial sequencing of the genome of these HEV-C isolates revealed considerable genetic diversity, mostly due to recombination. In the work presented herein, we carried out a more detailed characterization of the genomes of viruses from this collection. First, we determined the full VP1 sequence of 41 of these isolates of different types. These sequences were compared with those of HEV-C isolates obtained from other countries or in other contexts. The sequences of the Madagascan isolates of a given type formed specific clusters clearly differentiated from those formed by other strains of the same type isolated elsewhere. Second, we sequenced the entire genome of 10 viruses representing most of the lineages present in this panel. All but one of the genomes appeared to be mosaic assemblies of different genomic fragments generated by intra- and intertypic recombination. The location of the breakpoints suggested potential preferred genomic regions for recombination. Our results also suggest that recombination between type HEV-99 and other HEV-C may be quite rare. This first exhaustive genomic analysis of a panel of non-PV HEV-C cocirculating in a small human population highlights the high frequency of inter and intra-typic genetic recombination, constituting a widespread mechanism of genetic plasticity and continually shifting the HEV-C biodiversity.Maël BessaudMarie-Line JoffretBarbara HolmblatRichter RazafindratsimandresyFrancis DelpeyrouxPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24823 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maël Bessaud Marie-Line Joffret Barbara Holmblat Richter Razafindratsimandresy Francis Delpeyroux Genetic relationship between cocirculating Human enteroviruses species C.
description	Recombination events between human enteroviruses (HEV) are known to occur frequently and to participate in the evolution of these viruses. In a previous study, we reported the isolation of a panel of viruses belonging to the Human enterovirus species C (HEV-C) that had been cocirculating in a small geographic area of Madagascar in 2002. This panel included type 2 vaccine-derived polioviruses (PV) that had caused several cases of acute flaccid paralysis in humans. Previous partial sequencing of the genome of these HEV-C isolates revealed considerable genetic diversity, mostly due to recombination. In the work presented herein, we carried out a more detailed characterization of the genomes of viruses from this collection. First, we determined the full VP1 sequence of 41 of these isolates of different types. These sequences were compared with those of HEV-C isolates obtained from other countries or in other contexts. The sequences of the Madagascan isolates of a given type formed specific clusters clearly differentiated from those formed by other strains of the same type isolated elsewhere. Second, we sequenced the entire genome of 10 viruses representing most of the lineages present in this panel. All but one of the genomes appeared to be mosaic assemblies of different genomic fragments generated by intra- and intertypic recombination. The location of the breakpoints suggested potential preferred genomic regions for recombination. Our results also suggest that recombination between type HEV-99 and other HEV-C may be quite rare. This first exhaustive genomic analysis of a panel of non-PV HEV-C cocirculating in a small human population highlights the high frequency of inter and intra-typic genetic recombination, constituting a widespread mechanism of genetic plasticity and continually shifting the HEV-C biodiversity.
format	article
author	Maël Bessaud Marie-Line Joffret Barbara Holmblat Richter Razafindratsimandresy Francis Delpeyroux
author_facet	Maël Bessaud Marie-Line Joffret Barbara Holmblat Richter Razafindratsimandresy Francis Delpeyroux
author_sort	Maël Bessaud
title	Genetic relationship between cocirculating Human enteroviruses species C.
title_short	Genetic relationship between cocirculating Human enteroviruses species C.
title_full	Genetic relationship between cocirculating Human enteroviruses species C.
title_fullStr	Genetic relationship between cocirculating Human enteroviruses species C.
title_full_unstemmed	Genetic relationship between cocirculating Human enteroviruses species C.
title_sort	genetic relationship between cocirculating human enteroviruses species c.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/ed5c29fae7604bbc836c8f8163c5cb3a
work_keys_str_mv	AT maelbessaud geneticrelationshipbetweencocirculatinghumanenterovirusesspeciesc AT marielinejoffret geneticrelationshipbetweencocirculatinghumanenterovirusesspeciesc AT barbaraholmblat geneticrelationshipbetweencocirculatinghumanenterovirusesspeciesc AT richterrazafindratsimandresy geneticrelationshipbetweencocirculatinghumanenterovirusesspeciesc AT francisdelpeyroux geneticrelationshipbetweencocirculatinghumanenterovirusesspeciesc
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Genetic relationship between cocirculating Human enteroviruses species C.

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