Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina.

Mitochondria are descendants of the endosymbiotic α-proteobacterium most likely engulfed by the ancestral eukaryotic cells, and the proto-mitochondrial genome should have been severely streamlined in terms of both genome size and gene repertoire. In addition, mitochondrial (mt) sequence data indicat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuki Nishimura, Ryoma Kamikawa, Tetsuo Hashimoto, Yuji Inagaki
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed5c89c4307b40ebbea6435e986713db
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ed5c89c4307b40ebbea6435e986713db
record_format	dspace
spelling	oai:doaj.org-article:ed5c89c4307b40ebbea6435e986713db2021-11-18T07:19:07ZSeparate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina.1932-620310.1371/journal.pone.0037307https://doaj.org/article/ed5c89c4307b40ebbea6435e986713db2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22606358/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Mitochondria are descendants of the endosymbiotic α-proteobacterium most likely engulfed by the ancestral eukaryotic cells, and the proto-mitochondrial genome should have been severely streamlined in terms of both genome size and gene repertoire. In addition, mitochondrial (mt) sequence data indicated that frequent intron gain/loss events contributed to shaping the modern mt genome organizations, resulting in the homologous introns being shared between two distantly related mt genomes. Unfortunately, the bulk of mt sequence data currently available are of phylogenetically restricted lineages, i.e., metazoans, fungi, and land plants, and are insufficient to elucidate the entire picture of intron evolution in mt genomes. In this work, we sequenced a 12 kbp-fragment of the mt genome of the katablepharid Leucocryptos marina. Among nine protein-coding genes included in the mt genome fragment, the genes encoding cytochrome b and cytochrome c oxidase subunit I (cob and cox1) were interrupted by group I introns. We further identified that the cob and cox1 introns host open reading frames for homing endonucleases (HEs) belonging to distantly related superfamilies. Phylogenetic analyses recovered an affinity between the HE in the Leucocryptos cob intron and two green algal HEs, and that between the HE in the Leucocryptos cox1 intron and a fungal HE, suggesting that the Leucocryptos cob and cox1 introns possess distinct evolutionary origins. Although the current intron (and intronic HE) data are insufficient to infer how the homologous introns were distributed to distantly related mt genomes, the results presented here successfully expanded the evolutionary dynamism of group I introns in mt genomes.Yuki NishimuraRyoma KamikawaTetsuo HashimotoYuji InagakiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37307 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuki Nishimura Ryoma Kamikawa Tetsuo Hashimoto Yuji Inagaki Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina.
description	Mitochondria are descendants of the endosymbiotic α-proteobacterium most likely engulfed by the ancestral eukaryotic cells, and the proto-mitochondrial genome should have been severely streamlined in terms of both genome size and gene repertoire. In addition, mitochondrial (mt) sequence data indicated that frequent intron gain/loss events contributed to shaping the modern mt genome organizations, resulting in the homologous introns being shared between two distantly related mt genomes. Unfortunately, the bulk of mt sequence data currently available are of phylogenetically restricted lineages, i.e., metazoans, fungi, and land plants, and are insufficient to elucidate the entire picture of intron evolution in mt genomes. In this work, we sequenced a 12 kbp-fragment of the mt genome of the katablepharid Leucocryptos marina. Among nine protein-coding genes included in the mt genome fragment, the genes encoding cytochrome b and cytochrome c oxidase subunit I (cob and cox1) were interrupted by group I introns. We further identified that the cob and cox1 introns host open reading frames for homing endonucleases (HEs) belonging to distantly related superfamilies. Phylogenetic analyses recovered an affinity between the HE in the Leucocryptos cob intron and two green algal HEs, and that between the HE in the Leucocryptos cox1 intron and a fungal HE, suggesting that the Leucocryptos cob and cox1 introns possess distinct evolutionary origins. Although the current intron (and intronic HE) data are insufficient to infer how the homologous introns were distributed to distantly related mt genomes, the results presented here successfully expanded the evolutionary dynamism of group I introns in mt genomes.
format	article
author	Yuki Nishimura Ryoma Kamikawa Tetsuo Hashimoto Yuji Inagaki
author_facet	Yuki Nishimura Ryoma Kamikawa Tetsuo Hashimoto Yuji Inagaki
author_sort	Yuki Nishimura
title	Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina.
title_short	Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina.
title_full	Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina.
title_fullStr	Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina.
title_full_unstemmed	Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina.
title_sort	separate origins of group i introns in two mitochondrial genes of the katablepharid leucocryptos marina.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ed5c89c4307b40ebbea6435e986713db
work_keys_str_mv	AT yukinishimura separateoriginsofgroupiintronsintwomitochondrialgenesofthekatablepharidleucocryptosmarina AT ryomakamikawa separateoriginsofgroupiintronsintwomitochondrialgenesofthekatablepharidleucocryptosmarina AT tetsuohashimoto separateoriginsofgroupiintronsintwomitochondrialgenesofthekatablepharidleucocryptosmarina AT yujiinagaki separateoriginsofgroupiintronsintwomitochondrialgenesofthekatablepharidleucocryptosmarina
_version_	1718423636277198848

Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina.

Ejemplares similares