Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing

Abstract The black blow fly, Phormia regina (Meigen) (Diptera: Calliphoridae) is one of the most abundant carrion flies in North America. Calliphorids are important in agriculture and animal production, veterinary sciences, forensics and medical entomology. While the role of flies in the epidemiolog...

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Autores principales:	Jean M. Deguenon, Nicholas Travanty, Jiwei Zhu, Ann Carr, Steven Denning, Michael H. Reiskind, David W. Watson, R. Michael Roe, Loganathan Ponnusamy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed6b5f44df394811b67999fe3a9830b8
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spelling	oai:doaj.org-article:ed6b5f44df394811b67999fe3a9830b82021-12-02T15:12:22ZExogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing10.1038/s41598-019-56733-z2045-2322https://doaj.org/article/ed6b5f44df394811b67999fe3a9830b82019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-56733-zhttps://doaj.org/toc/2045-2322Abstract The black blow fly, Phormia regina (Meigen) (Diptera: Calliphoridae) is one of the most abundant carrion flies in North America. Calliphorids are important in agriculture and animal production, veterinary sciences, forensics and medical entomology. While the role of flies in the epidemiology of human and animal diseases is an active area of research, little is known about the microorganisms associated with these insects. We examined the diversity of wild-caught black blow fly endogenous (internal body) and exogenous (external body) microbial communities using 16S rRNA gene sequencing. Overall, 27 phyla, 171 families and 533 genera were detected, and diversity was significantly higher (P < 0.05) on external body surfaces. At the genus level, Dysgonomonas, Ignatzschineria, Acinetobacter, Vagococcus, Myroides, and Wohlfahrtiimonas were predominant. Cloning and sequencing of nearly full-length fragments of the 16S rRNA gene showed that some of the species identified are known to be pathogenic to humans, animals, and plants. Myroides odoratimimus and Acinetobacter radioresistens are well-known, multi-drug resistant bacteria. These results provide a snapshot of the microbial communities harbored by adult black blow flies and call for more comprehensive studies to better characterize the role these flies may play in the transmission of pathogenic microorganisms.Jean M. DeguenonNicholas TravantyJiwei ZhuAnn CarrSteven DenningMichael H. ReiskindDavid W. WatsonR. Michael RoeLoganathan PonnusamyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jean M. Deguenon Nicholas Travanty Jiwei Zhu Ann Carr Steven Denning Michael H. Reiskind David W. Watson R. Michael Roe Loganathan Ponnusamy Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing
description	Abstract The black blow fly, Phormia regina (Meigen) (Diptera: Calliphoridae) is one of the most abundant carrion flies in North America. Calliphorids are important in agriculture and animal production, veterinary sciences, forensics and medical entomology. While the role of flies in the epidemiology of human and animal diseases is an active area of research, little is known about the microorganisms associated with these insects. We examined the diversity of wild-caught black blow fly endogenous (internal body) and exogenous (external body) microbial communities using 16S rRNA gene sequencing. Overall, 27 phyla, 171 families and 533 genera were detected, and diversity was significantly higher (P < 0.05) on external body surfaces. At the genus level, Dysgonomonas, Ignatzschineria, Acinetobacter, Vagococcus, Myroides, and Wohlfahrtiimonas were predominant. Cloning and sequencing of nearly full-length fragments of the 16S rRNA gene showed that some of the species identified are known to be pathogenic to humans, animals, and plants. Myroides odoratimimus and Acinetobacter radioresistens are well-known, multi-drug resistant bacteria. These results provide a snapshot of the microbial communities harbored by adult black blow flies and call for more comprehensive studies to better characterize the role these flies may play in the transmission of pathogenic microorganisms.
format	article
author	Jean M. Deguenon Nicholas Travanty Jiwei Zhu Ann Carr Steven Denning Michael H. Reiskind David W. Watson R. Michael Roe Loganathan Ponnusamy
author_facet	Jean M. Deguenon Nicholas Travanty Jiwei Zhu Ann Carr Steven Denning Michael H. Reiskind David W. Watson R. Michael Roe Loganathan Ponnusamy
author_sort	Jean M. Deguenon
title	Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing
title_short	Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing
title_full	Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing
title_fullStr	Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing
title_full_unstemmed	Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing
title_sort	exogenous and endogenous microbiomes of wild-caught phormia regina (diptera: calliphoridae) flies from a suburban farm by 16s rrna gene sequencing
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ed6b5f44df394811b67999fe3a9830b8
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Exogenous and endogenous microbiomes of wild-caught Phormia regina (Diptera: Calliphoridae) flies from a suburban farm by 16S rRNA gene sequencing

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