A role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.

Methyl-CpG-binding protein 2 (MeCP2) is generally considered to act as a transcriptional repressor, whereas recent studies suggest that MeCP2 is also involved in transcription activation. To gain insight into this dual function of MeCP2, we assessed the impact of MeCP2 on higher-order chromatin stru...

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Autores principales:	Maartje C Brink, Diewertje G E Piebes, Marloes L de Groote, Martijn S Luijsterburg, Corella S Casas-Delucchi, Roel van Driel, Marianne G Rots, M Cristina Cardoso, Pernette J Verschure
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed6fb092cce4456897a64f41a187c22f
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spelling	oai:doaj.org-article:ed6fb092cce4456897a64f41a187c22f2021-11-18T09:03:18ZA role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.1932-620310.1371/journal.pone.0069347https://doaj.org/article/ed6fb092cce4456897a64f41a187c22f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23935992/?tool=EBIhttps://doaj.org/toc/1932-6203Methyl-CpG-binding protein 2 (MeCP2) is generally considered to act as a transcriptional repressor, whereas recent studies suggest that MeCP2 is also involved in transcription activation. To gain insight into this dual function of MeCP2, we assessed the impact of MeCP2 on higher-order chromatin structure in living cells using mammalian cell systems harbouring a lactose operator and reporter gene-containing chromosomal domain to assess the effect of lactose repressor-tagged MeCP2 (and separate MeCP2 domains) binding in living cells. Our data reveal that targeted binding of MeCP2 elicits extensive chromatin unfolding. MeCP2-induced chromatin unfolding is triggered independently of the methyl-cytosine-binding domain. Interestingly, MeCP2 binding triggers the loss of HP1γ at the chromosomal domain and an increased HP1γ mobility, which is not observed for HP1α and HP1β. Surprisingly, MeCP2-induced chromatin unfolding is not associated with transcriptional activation. Our study suggests a novel role for MeCP2 in reorganizing chromatin to facilitate a switch in gene activity.Maartje C BrinkDiewertje G E PiebesMarloes L de GrooteMartijn S LuijsterburgCorella S Casas-DelucchiRoel van DrielMarianne G RotsM Cristina CardosoPernette J VerschurePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e69347 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maartje C Brink Diewertje G E Piebes Marloes L de Groote Martijn S Luijsterburg Corella S Casas-Delucchi Roel van Driel Marianne G Rots M Cristina Cardoso Pernette J Verschure A role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.
description	Methyl-CpG-binding protein 2 (MeCP2) is generally considered to act as a transcriptional repressor, whereas recent studies suggest that MeCP2 is also involved in transcription activation. To gain insight into this dual function of MeCP2, we assessed the impact of MeCP2 on higher-order chromatin structure in living cells using mammalian cell systems harbouring a lactose operator and reporter gene-containing chromosomal domain to assess the effect of lactose repressor-tagged MeCP2 (and separate MeCP2 domains) binding in living cells. Our data reveal that targeted binding of MeCP2 elicits extensive chromatin unfolding. MeCP2-induced chromatin unfolding is triggered independently of the methyl-cytosine-binding domain. Interestingly, MeCP2 binding triggers the loss of HP1γ at the chromosomal domain and an increased HP1γ mobility, which is not observed for HP1α and HP1β. Surprisingly, MeCP2-induced chromatin unfolding is not associated with transcriptional activation. Our study suggests a novel role for MeCP2 in reorganizing chromatin to facilitate a switch in gene activity.
format	article
author	Maartje C Brink Diewertje G E Piebes Marloes L de Groote Martijn S Luijsterburg Corella S Casas-Delucchi Roel van Driel Marianne G Rots M Cristina Cardoso Pernette J Verschure
author_facet	Maartje C Brink Diewertje G E Piebes Marloes L de Groote Martijn S Luijsterburg Corella S Casas-Delucchi Roel van Driel Marianne G Rots M Cristina Cardoso Pernette J Verschure
author_sort	Maartje C Brink
title	A role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.
title_short	A role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.
title_full	A role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.
title_fullStr	A role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.
title_full_unstemmed	A role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.
title_sort	role for mecp2 in switching gene activity via chromatin unfolding and hp1γ displacement.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ed6fb092cce4456897a64f41a187c22f
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A role for MeCP2 in switching gene activity via chromatin unfolding and HP1γ displacement.

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