Overexpression of ZNT1 and NRAMP4 from the Ni Hyperaccumulator Noccaea caerulescens Population Monte Prinzera in Arabidopsis thaliana Perturbs Fe, Mn, and Ni Accumulation

Metalliferous soils are characterized by a high content of metal compounds that can hamper plant growth. The pseudometallophyte Noccaea caerulescens is able to grow on metalliferous substrates by implementing both tolerance and accumulation of usually toxic metal ions. Expression...

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Auteurs principaux:	Elisa Fasani, Giovanni DalCorso, Gianluca Zorzi, Caterina Agrimonti, Rosaria Fragni, Giovanna Visioli, Antonella Furini
Format:	article
Langue:	EN
Publié:	MDPI AG 2021
Sujets:	heavy metals hyperaccumulation <i>Noccaea caerulescens</i> ecotype Monte Prinzera heavy metal accumulation in plants Biology (General) QH301-705.5 Chemistry QD1-999
Accès en ligne:	https://doaj.org/article/ed7f69f72e9745f48d586f37ea2a598d
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spelling	oai:doaj.org-article:ed7f69f72e9745f48d586f37ea2a598d2021-11-11T17:18:42ZOverexpression of ZNT1 and NRAMP4 from the Ni Hyperaccumulator <i>Noccaea caerulescens</i> Population Monte Prinzera in <i>Arabidopsis thaliana</i> Perturbs Fe, Mn, and Ni Accumulation10.3390/ijms2221118961422-00671661-6596https://doaj.org/article/ed7f69f72e9745f48d586f37ea2a598d2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11896https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Metalliferous soils are characterized by a high content of metal compounds that can hamper plant growth. The pseudometallophyte <i>Noccaea caerulescens</i> is able to grow on metalliferous substrates by implementing both tolerance and accumulation of usually toxic metal ions. Expression of particular transmembrane transporter proteins (e.g., members of the ZIP and NRAMP families) leads to metal tolerance and accumulation, and its comparison between hyperaccumulator <i>N. caerulescens</i> with non-accumulator relatives <i>Arabidopsis thaliana</i> and <i>Thlaspi arvense</i> has deepened our knowledge on mechanisms adopted by plants to survive in metalliferous soils. In this work, two transporters, <i>ZNT1</i> and <i>NRAMP4,</i> expressed in a serpentinic population of <i>N. caerulescens</i> identified on the Monte Prinzera (Italy) are considered, and their expression has been induced in yeast and in <i>A. thaliana</i>. In the latter, single transgenic lines were crossed to test the effect of the combined over-expression of the two transporters. An enhanced iron and manganese translocation towards the shoot was induced by overexpression of <i>NcZNT1</i>. The combined overexpression of <i>NcZNT1</i> and <i>NcNRAMP4</i> did perturb the metal accumulation in plants.Elisa FasaniGiovanni DalCorsoGianluca ZorziCaterina AgrimontiRosaria FragniGiovanna VisioliAntonella FuriniMDPI AGarticleheavy metalshyperaccumulation<i>Noccaea caerulescens</i> ecotype Monte Prinzeraheavy metal accumulation in plantsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11896, p 11896 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	heavy metals hyperaccumulation <i>Noccaea caerulescens</i> ecotype Monte Prinzera heavy metal accumulation in plants Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	heavy metals hyperaccumulation <i>Noccaea caerulescens</i> ecotype Monte Prinzera heavy metal accumulation in plants Biology (General) QH301-705.5 Chemistry QD1-999 Elisa Fasani Giovanni DalCorso Gianluca Zorzi Caterina Agrimonti Rosaria Fragni Giovanna Visioli Antonella Furini Overexpression of ZNT1 and NRAMP4 from the Ni Hyperaccumulator <i>Noccaea caerulescens</i> Population Monte Prinzera in <i>Arabidopsis thaliana</i> Perturbs Fe, Mn, and Ni Accumulation
description	Metalliferous soils are characterized by a high content of metal compounds that can hamper plant growth. The pseudometallophyte <i>Noccaea caerulescens</i> is able to grow on metalliferous substrates by implementing both tolerance and accumulation of usually toxic metal ions. Expression of particular transmembrane transporter proteins (e.g., members of the ZIP and NRAMP families) leads to metal tolerance and accumulation, and its comparison between hyperaccumulator <i>N. caerulescens</i> with non-accumulator relatives <i>Arabidopsis thaliana</i> and <i>Thlaspi arvense</i> has deepened our knowledge on mechanisms adopted by plants to survive in metalliferous soils. In this work, two transporters, <i>ZNT1</i> and <i>NRAMP4,</i> expressed in a serpentinic population of <i>N. caerulescens</i> identified on the Monte Prinzera (Italy) are considered, and their expression has been induced in yeast and in <i>A. thaliana</i>. In the latter, single transgenic lines were crossed to test the effect of the combined over-expression of the two transporters. An enhanced iron and manganese translocation towards the shoot was induced by overexpression of <i>NcZNT1</i>. The combined overexpression of <i>NcZNT1</i> and <i>NcNRAMP4</i> did perturb the metal accumulation in plants.
format	article
author	Elisa Fasani Giovanni DalCorso Gianluca Zorzi Caterina Agrimonti Rosaria Fragni Giovanna Visioli Antonella Furini
author_facet	Elisa Fasani Giovanni DalCorso Gianluca Zorzi Caterina Agrimonti Rosaria Fragni Giovanna Visioli Antonella Furini
author_sort	Elisa Fasani
title	Overexpression of ZNT1 and NRAMP4 from the Ni Hyperaccumulator <i>Noccaea caerulescens</i> Population Monte Prinzera in <i>Arabidopsis thaliana</i> Perturbs Fe, Mn, and Ni Accumulation
title_short	Overexpression of ZNT1 and NRAMP4 from the Ni Hyperaccumulator <i>Noccaea caerulescens</i> Population Monte Prinzera in <i>Arabidopsis thaliana</i> Perturbs Fe, Mn, and Ni Accumulation
title_full	Overexpression of ZNT1 and NRAMP4 from the Ni Hyperaccumulator <i>Noccaea caerulescens</i> Population Monte Prinzera in <i>Arabidopsis thaliana</i> Perturbs Fe, Mn, and Ni Accumulation
title_fullStr	Overexpression of ZNT1 and NRAMP4 from the Ni Hyperaccumulator <i>Noccaea caerulescens</i> Population Monte Prinzera in <i>Arabidopsis thaliana</i> Perturbs Fe, Mn, and Ni Accumulation
title_full_unstemmed	Overexpression of ZNT1 and NRAMP4 from the Ni Hyperaccumulator <i>Noccaea caerulescens</i> Population Monte Prinzera in <i>Arabidopsis thaliana</i> Perturbs Fe, Mn, and Ni Accumulation
title_sort	overexpression of znt1 and nramp4 from the ni hyperaccumulator <i>noccaea caerulescens</i> population monte prinzera in <i>arabidopsis thaliana</i> perturbs fe, mn, and ni accumulation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ed7f69f72e9745f48d586f37ea2a598d
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Overexpression of ZNT1 and NRAMP4 from the Ni Hyperaccumulator <i>Noccaea caerulescens</i> Population Monte Prinzera in <i>Arabidopsis thaliana</i> Perturbs Fe, Mn, and Ni Accumulation

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