Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells

Bone mesenchymal stem cells (BMSCs) have been used for the treatment of acute uterine injury (AUI)-induced intrauterine adhesion (IUA) via interacting with the endothelial progenitor cells (EPCs), and BMSCs-derived exosomes (BMSCs-exo) may be the key regulators for this process. However, the underly...

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Autores principales:	Yana Liu, Shihong Zhang, Zhiwei Xue, Xiaoxia Zhou, Lin Tong, Jiachen Liao, Huan Pan, Shu Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	bone mesenchymal stem cells endothelial progenitor cells intrauterine adhesion mir-223-3p nlrp3-mediated pyroptotic cell death Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/ed81d2433baf4bf88e0334198f8b91bd
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spelling	oai:doaj.org-article:ed81d2433baf4bf88e0334198f8b91bd2021-11-11T14:23:43ZBone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells2165-59792165-598710.1080/21655979.2021.2001185https://doaj.org/article/ed81d2433baf4bf88e0334198f8b91bd2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2001185https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Bone mesenchymal stem cells (BMSCs) have been used for the treatment of acute uterine injury (AUI)-induced intrauterine adhesion (IUA) via interacting with the endothelial progenitor cells (EPCs), and BMSCs-derived exosomes (BMSCs-exo) may be the key regulators for this process. However, the underlying mechanisms have not been studied. Based on the existed literatures, lipopolysaccharide (LPS) was used to induce AUI in mice models and EPCs to mimic the realistic pathogenesis of IUA in vivo and in vitro. Our data suggested that LPS induced apoptotic and pyroptotic cell death in mice uterine horn tissues and EPCs, and the clinical data supported that increased levels of pro-inflammatory cytokines IL-18 and IL-1β were also observed in IUA patients’ serum samples, and silencing of NLRP3 rescued cell viability in LPS-treated EPCs. Next, the LPS-treated EPCs were respectively co-cultured with BMSCs in the Transwell system and BMSCs-exo, and the results hinted that both BMSCs and BMSCs-exo reversed the promoting effects of LPS treatment-induced cell death in EPCs. Then, we screened out miR-223-3p, as the upstream regulator for NLRP3, was enriched in BMSCs-exo, and BMSCs-exo inactivated NLRP3-mediated cell pyroptosis in EPCs via delivering miR-223-3p. Interestingly, upregulation of miR-223-3p attenuated LPS-induced cell death in EPCs. Collectively, we concluded that BMSCs-exo upregulated miR-223-3p to degrade NLRP3 in EPCs, which further reversed the cytotoxic effects of LPS treatment on EPCs to ameliorate LPS-induced AUI.Yana LiuShihong ZhangZhiwei XueXiaoxia ZhouLin TongJiachen LiaoHuan PanShu ZhouTaylor & Francis Grouparticlebone mesenchymal stem cellsendothelial progenitor cellsintrauterine adhesionmir-223-3pnlrp3-mediated pyroptotic cell deathBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bone mesenchymal stem cells endothelial progenitor cells intrauterine adhesion mir-223-3p nlrp3-mediated pyroptotic cell death Biotechnology TP248.13-248.65
spellingShingle	bone mesenchymal stem cells endothelial progenitor cells intrauterine adhesion mir-223-3p nlrp3-mediated pyroptotic cell death Biotechnology TP248.13-248.65 Yana Liu Shihong Zhang Zhiwei Xue Xiaoxia Zhou Lin Tong Jiachen Liao Huan Pan Shu Zhou Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells
description	Bone mesenchymal stem cells (BMSCs) have been used for the treatment of acute uterine injury (AUI)-induced intrauterine adhesion (IUA) via interacting with the endothelial progenitor cells (EPCs), and BMSCs-derived exosomes (BMSCs-exo) may be the key regulators for this process. However, the underlying mechanisms have not been studied. Based on the existed literatures, lipopolysaccharide (LPS) was used to induce AUI in mice models and EPCs to mimic the realistic pathogenesis of IUA in vivo and in vitro. Our data suggested that LPS induced apoptotic and pyroptotic cell death in mice uterine horn tissues and EPCs, and the clinical data supported that increased levels of pro-inflammatory cytokines IL-18 and IL-1β were also observed in IUA patients’ serum samples, and silencing of NLRP3 rescued cell viability in LPS-treated EPCs. Next, the LPS-treated EPCs were respectively co-cultured with BMSCs in the Transwell system and BMSCs-exo, and the results hinted that both BMSCs and BMSCs-exo reversed the promoting effects of LPS treatment-induced cell death in EPCs. Then, we screened out miR-223-3p, as the upstream regulator for NLRP3, was enriched in BMSCs-exo, and BMSCs-exo inactivated NLRP3-mediated cell pyroptosis in EPCs via delivering miR-223-3p. Interestingly, upregulation of miR-223-3p attenuated LPS-induced cell death in EPCs. Collectively, we concluded that BMSCs-exo upregulated miR-223-3p to degrade NLRP3 in EPCs, which further reversed the cytotoxic effects of LPS treatment on EPCs to ameliorate LPS-induced AUI.
format	article
author	Yana Liu Shihong Zhang Zhiwei Xue Xiaoxia Zhou Lin Tong Jiachen Liao Huan Pan Shu Zhou
author_facet	Yana Liu Shihong Zhang Zhiwei Xue Xiaoxia Zhou Lin Tong Jiachen Liao Huan Pan Shu Zhou
author_sort	Yana Liu
title	Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells
title_short	Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells
title_full	Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells
title_fullStr	Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells
title_full_unstemmed	Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells
title_sort	bone mesenchymal stem cells-derived mir-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/ed81d2433baf4bf88e0334198f8b91bd
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Bone mesenchymal stem cells-derived miR-223-3p-containing exosomes ameliorate lipopolysaccharide-induced acute uterine injury via interacting with endothelial progenitor cells

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