Encapsulation of α-Pinene in Delivery Systems Based on Liposomes and Cyclodextrins

The essential oil component α-pinene has multiple biological activities. However, its application is limited owing to its volatility, low aqueous solubility, and chemical instability. For the aim of improving its physicochemical properties, α-pinene was encapsulated in conventional liposomes (CLs) a...

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Autores principales:	Zahraa Hammoud, Maya Kayouka, Adriana Trifan, Elwira Sieniawska, Jouda Mediouni Ben Jemâa, Abdelhamid Elaissari, Hélène Greige-Gerges
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	α-pinene drug-in-cyclodextrin-in-liposomes encapsulation hydroxypropyl-β-cyclodextrin liposomes Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/ed8285fd22f241bea79bc7fd7bc45e05
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spelling	oai:doaj.org-article:ed8285fd22f241bea79bc7fd7bc45e052021-11-25T18:27:39ZEncapsulation of α-Pinene in Delivery Systems Based on Liposomes and Cyclodextrins10.3390/molecules262268401420-3049https://doaj.org/article/ed8285fd22f241bea79bc7fd7bc45e052021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6840https://doaj.org/toc/1420-3049The essential oil component α-pinene has multiple biological activities. However, its application is limited owing to its volatility, low aqueous solubility, and chemical instability. For the aim of improving its physicochemical properties, α-pinene was encapsulated in conventional liposomes (CLs) and drug-in-cyclodextrin-in-liposomes (DCLs). Hydroxypropyl-β-cyclodextrin/α-pinene (HP-β-CD/α-pinene) inclusion complexes were prepared in aqueous solution, and the optimal solubilization of α-pinene occurred at HP-β-CD:α-pinene molar ratio of 7.5:1. The ethanol-injection method was applied to produce different formulations using saturated (Phospholipon 90H) or unsaturated (Lipoid S100) phospholipids in combination with cholesterol. The size, the phospholipid and cholesterol incorporation rates, the encapsulation efficiency (EE), and the loading rate (LR) of α-pinene were determined, and the storage stability of liposomes was assessed. The results showed that α-pinene was efficiently entrapped in CLs and DCLs with high EE values. Moreover, Lipoid S100 CLs displayed the highest LR (22.9 ± 2.2%) of α-pinene compared to the other formulations. Both carrier systems HP-β-CD/α-pinene inclusion complex and Lipoid S100 CLs presented a gradual release of α-pinene. Furthermore, the DPPH radical scavenging activity of α-pinene was maintained upon encapsulation in Lipoid S100 CLs. Finally, it was found that all formulations were stable after three months of storage at 4 °C.Zahraa HammoudMaya KayoukaAdriana TrifanElwira SieniawskaJouda Mediouni Ben JemâaAbdelhamid ElaissariHélène Greige-GergesMDPI AGarticleα-pinenedrug-in-cyclodextrin-in-liposomesencapsulationhydroxypropyl-β-cyclodextrinliposomesOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6840, p 6840 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	α-pinene drug-in-cyclodextrin-in-liposomes encapsulation hydroxypropyl-β-cyclodextrin liposomes Organic chemistry QD241-441
spellingShingle	α-pinene drug-in-cyclodextrin-in-liposomes encapsulation hydroxypropyl-β-cyclodextrin liposomes Organic chemistry QD241-441 Zahraa Hammoud Maya Kayouka Adriana Trifan Elwira Sieniawska Jouda Mediouni Ben Jemâa Abdelhamid Elaissari Hélène Greige-Gerges Encapsulation of α-Pinene in Delivery Systems Based on Liposomes and Cyclodextrins
description	The essential oil component α-pinene has multiple biological activities. However, its application is limited owing to its volatility, low aqueous solubility, and chemical instability. For the aim of improving its physicochemical properties, α-pinene was encapsulated in conventional liposomes (CLs) and drug-in-cyclodextrin-in-liposomes (DCLs). Hydroxypropyl-β-cyclodextrin/α-pinene (HP-β-CD/α-pinene) inclusion complexes were prepared in aqueous solution, and the optimal solubilization of α-pinene occurred at HP-β-CD:α-pinene molar ratio of 7.5:1. The ethanol-injection method was applied to produce different formulations using saturated (Phospholipon 90H) or unsaturated (Lipoid S100) phospholipids in combination with cholesterol. The size, the phospholipid and cholesterol incorporation rates, the encapsulation efficiency (EE), and the loading rate (LR) of α-pinene were determined, and the storage stability of liposomes was assessed. The results showed that α-pinene was efficiently entrapped in CLs and DCLs with high EE values. Moreover, Lipoid S100 CLs displayed the highest LR (22.9 ± 2.2%) of α-pinene compared to the other formulations. Both carrier systems HP-β-CD/α-pinene inclusion complex and Lipoid S100 CLs presented a gradual release of α-pinene. Furthermore, the DPPH radical scavenging activity of α-pinene was maintained upon encapsulation in Lipoid S100 CLs. Finally, it was found that all formulations were stable after three months of storage at 4 °C.
format	article
author	Zahraa Hammoud Maya Kayouka Adriana Trifan Elwira Sieniawska Jouda Mediouni Ben Jemâa Abdelhamid Elaissari Hélène Greige-Gerges
author_facet	Zahraa Hammoud Maya Kayouka Adriana Trifan Elwira Sieniawska Jouda Mediouni Ben Jemâa Abdelhamid Elaissari Hélène Greige-Gerges
author_sort	Zahraa Hammoud
title	Encapsulation of α-Pinene in Delivery Systems Based on Liposomes and Cyclodextrins
title_short	Encapsulation of α-Pinene in Delivery Systems Based on Liposomes and Cyclodextrins
title_full	Encapsulation of α-Pinene in Delivery Systems Based on Liposomes and Cyclodextrins
title_fullStr	Encapsulation of α-Pinene in Delivery Systems Based on Liposomes and Cyclodextrins
title_full_unstemmed	Encapsulation of α-Pinene in Delivery Systems Based on Liposomes and Cyclodextrins
title_sort	encapsulation of α-pinene in delivery systems based on liposomes and cyclodextrins
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ed8285fd22f241bea79bc7fd7bc45e05
work_keys_str_mv	AT zahraahammoud encapsulationofapineneindeliverysystemsbasedonliposomesandcyclodextrins AT mayakayouka encapsulationofapineneindeliverysystemsbasedonliposomesandcyclodextrins AT adrianatrifan encapsulationofapineneindeliverysystemsbasedonliposomesandcyclodextrins AT elwirasieniawska encapsulationofapineneindeliverysystemsbasedonliposomesandcyclodextrins AT joudamediounibenjemaa encapsulationofapineneindeliverysystemsbasedonliposomesandcyclodextrins AT abdelhamidelaissari encapsulationofapineneindeliverysystemsbasedonliposomesandcyclodextrins AT helenegreigegerges encapsulationofapineneindeliverysystemsbasedonliposomesandcyclodextrins
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Encapsulation of α-Pinene in Delivery Systems Based on Liposomes and Cyclodextrins

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