Variances in the Expression of mRNAs and miRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer

Research has indicated higher concentrations of histamine and polyamine in endometrioid tissue in comparison with healthy tissue. The aim of this study was to evaluate changes in the expression patterns of messenger RNA (mRNAs) and microRNA (miRNAs) related to the histaminergic system in endometrial...

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Autores principales:	Michał Czerwiński, Anna Bednarska-Czerwińska, Paweł Ordon, Magdalena Gradzik, Marcin Oplawski, Dariusz Boroń, Hanna Zientek, Oskar Ogloszka, Beniamin Oskar Grabarek
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	endometrioid endometrial cancer grading microRNA histaminergic system molecular marker Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ed84f8afada04594a65174da611e7192
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spelling	oai:doaj.org-article:ed84f8afada04594a65174da611e71922021-11-25T16:48:41ZVariances in the Expression of mRNAs and miRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer10.3390/biomedicines91115352227-9059https://doaj.org/article/ed84f8afada04594a65174da611e71922021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1535https://doaj.org/toc/2227-9059Research has indicated higher concentrations of histamine and polyamine in endometrioid tissue in comparison with healthy tissue. The aim of this study was to evaluate changes in the expression patterns of messenger RNA (mRNAs) and microRNA (miRNAs) related to the histaminergic system in endometrial samples and whole blood in women with endometrioid endometrial cancer. The study group consisted of 30 women with endometrioid endometrial cancer qualified for hysterectomy (G1 well-differentiated, 15 cases; G2 moderately differentiated, 8 cases; and G3 poorly differentiated, 7 cases). The control group included 30 women with no neoplastic changes during routine gynecological examinations. The molecular analysis consisted of the microarray analysis of mRNAs and miRNAs related to the histaminergic system, reverse-transcription quantitative polymerase chain reaction (RTqPCR), and enzyme-linked immunosorbent assay (ELISA). Out of 65 mRNAs connected with the histaminergic system, 10 differentiate the samples of tissue and blood obtained from patients with endometrioid endometrial cancer in comparison with the control group (<i>p</i> < 0.05). mRNA histamine receptor 1,3 (<i>HRH1</i>, <i>HRH3</i>), and solute carrier family 22 member 3 (<i>SLC23A2</i>) differentiating samples of endometrioid endometrial cancer independent of either G or control. The highest probability of interaction, based on the target score miRDB, between the selected miRNAs and mRNAs was found for the hybrids hsa-miR-1-3p and endothelin 1 (<i>END1</i>), hsa-miR-27a-5β and <i>SLC23A2</i>. The selected mRNA and miRNA transcripts seem to be promising for molecularly targeted therapies in the context of endometrioid endometrial cancer.Michał CzerwińskiAnna Bednarska-CzerwińskaPaweł OrdonMagdalena GradzikMarcin OplawskiDariusz BorońHanna ZientekOskar OgloszkaBeniamin Oskar GrabarekMDPI AGarticleendometrioid endometrial cancergradingmicroRNAhistaminergic systemmolecular markerBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1535, p 1535 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endometrioid endometrial cancer grading microRNA histaminergic system molecular marker Biology (General) QH301-705.5
spellingShingle	endometrioid endometrial cancer grading microRNA histaminergic system molecular marker Biology (General) QH301-705.5 Michał Czerwiński Anna Bednarska-Czerwińska Paweł Ordon Magdalena Gradzik Marcin Oplawski Dariusz Boroń Hanna Zientek Oskar Ogloszka Beniamin Oskar Grabarek Variances in the Expression of mRNAs and miRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer
description	Research has indicated higher concentrations of histamine and polyamine in endometrioid tissue in comparison with healthy tissue. The aim of this study was to evaluate changes in the expression patterns of messenger RNA (mRNAs) and microRNA (miRNAs) related to the histaminergic system in endometrial samples and whole blood in women with endometrioid endometrial cancer. The study group consisted of 30 women with endometrioid endometrial cancer qualified for hysterectomy (G1 well-differentiated, 15 cases; G2 moderately differentiated, 8 cases; and G3 poorly differentiated, 7 cases). The control group included 30 women with no neoplastic changes during routine gynecological examinations. The molecular analysis consisted of the microarray analysis of mRNAs and miRNAs related to the histaminergic system, reverse-transcription quantitative polymerase chain reaction (RTqPCR), and enzyme-linked immunosorbent assay (ELISA). Out of 65 mRNAs connected with the histaminergic system, 10 differentiate the samples of tissue and blood obtained from patients with endometrioid endometrial cancer in comparison with the control group (<i>p</i> < 0.05). mRNA histamine receptor 1,3 (<i>HRH1</i>, <i>HRH3</i>), and solute carrier family 22 member 3 (<i>SLC23A2</i>) differentiating samples of endometrioid endometrial cancer independent of either G or control. The highest probability of interaction, based on the target score miRDB, between the selected miRNAs and mRNAs was found for the hybrids hsa-miR-1-3p and endothelin 1 (<i>END1</i>), hsa-miR-27a-5β and <i>SLC23A2</i>. The selected mRNA and miRNA transcripts seem to be promising for molecularly targeted therapies in the context of endometrioid endometrial cancer.
format	article
author	Michał Czerwiński Anna Bednarska-Czerwińska Paweł Ordon Magdalena Gradzik Marcin Oplawski Dariusz Boroń Hanna Zientek Oskar Ogloszka Beniamin Oskar Grabarek
author_facet	Michał Czerwiński Anna Bednarska-Czerwińska Paweł Ordon Magdalena Gradzik Marcin Oplawski Dariusz Boroń Hanna Zientek Oskar Ogloszka Beniamin Oskar Grabarek
author_sort	Michał Czerwiński
title	Variances in the Expression of mRNAs and miRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer
title_short	Variances in the Expression of mRNAs and miRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer
title_full	Variances in the Expression of mRNAs and miRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer
title_fullStr	Variances in the Expression of mRNAs and miRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer
title_full_unstemmed	Variances in the Expression of mRNAs and miRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer
title_sort	variances in the expression of mrnas and mirnas related to the histaminergic system in endometrioid endometrial cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ed84f8afada04594a65174da611e7192
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Variances in the Expression of mRNAs and miRNAs Related to the Histaminergic System in Endometrioid Endometrial Cancer

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