Flexibility of a eukaryotic lipidome--insights from yeast lipidomics.

Mass spectrometry-based shotgun lipidomics has enabled the quantitative and comprehensive assessment of cellular lipid compositions. The yeast Saccharomyces cerevisiae has proven to be a particularly valuable experimental system for studying lipid-related cellular processes. Here, by applying our sh...

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Autores principales:	Christian Klose, Michal A Surma, Mathias J Gerl, Felix Meyenhofer, Andrej Shevchenko, Kai Simons
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed88b3b0e3264d01a5c7f84b3dea3c6d
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spelling	oai:doaj.org-article:ed88b3b0e3264d01a5c7f84b3dea3c6d2021-11-18T07:21:47ZFlexibility of a eukaryotic lipidome--insights from yeast lipidomics.1932-620310.1371/journal.pone.0035063https://doaj.org/article/ed88b3b0e3264d01a5c7f84b3dea3c6d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22529973/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Mass spectrometry-based shotgun lipidomics has enabled the quantitative and comprehensive assessment of cellular lipid compositions. The yeast Saccharomyces cerevisiae has proven to be a particularly valuable experimental system for studying lipid-related cellular processes. Here, by applying our shotgun lipidomics platform, we investigated the influence of a variety of commonly used growth conditions on the yeast lipidome, including glycerophospholipids, triglycerides, ergosterol as well as complex sphingolipids. This extensive dataset allowed for a quantitative description of the intrinsic flexibility of a eukaryotic lipidome, thereby providing new insights into the adjustments of lipid biosynthetic pathways. In addition, we established a baseline for future lipidomic experiments in yeast. Finally, flexibility of lipidomic features is proposed as a new parameter for the description of the physiological state of an organism.Christian KloseMichal A SurmaMathias J GerlFelix MeyenhoferAndrej ShevchenkoKai SimonsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35063 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Klose Michal A Surma Mathias J Gerl Felix Meyenhofer Andrej Shevchenko Kai Simons Flexibility of a eukaryotic lipidome--insights from yeast lipidomics.
description	Mass spectrometry-based shotgun lipidomics has enabled the quantitative and comprehensive assessment of cellular lipid compositions. The yeast Saccharomyces cerevisiae has proven to be a particularly valuable experimental system for studying lipid-related cellular processes. Here, by applying our shotgun lipidomics platform, we investigated the influence of a variety of commonly used growth conditions on the yeast lipidome, including glycerophospholipids, triglycerides, ergosterol as well as complex sphingolipids. This extensive dataset allowed for a quantitative description of the intrinsic flexibility of a eukaryotic lipidome, thereby providing new insights into the adjustments of lipid biosynthetic pathways. In addition, we established a baseline for future lipidomic experiments in yeast. Finally, flexibility of lipidomic features is proposed as a new parameter for the description of the physiological state of an organism.
format	article
author	Christian Klose Michal A Surma Mathias J Gerl Felix Meyenhofer Andrej Shevchenko Kai Simons
author_facet	Christian Klose Michal A Surma Mathias J Gerl Felix Meyenhofer Andrej Shevchenko Kai Simons
author_sort	Christian Klose
title	Flexibility of a eukaryotic lipidome--insights from yeast lipidomics.
title_short	Flexibility of a eukaryotic lipidome--insights from yeast lipidomics.
title_full	Flexibility of a eukaryotic lipidome--insights from yeast lipidomics.
title_fullStr	Flexibility of a eukaryotic lipidome--insights from yeast lipidomics.
title_full_unstemmed	Flexibility of a eukaryotic lipidome--insights from yeast lipidomics.
title_sort	flexibility of a eukaryotic lipidome--insights from yeast lipidomics.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ed88b3b0e3264d01a5c7f84b3dea3c6d
work_keys_str_mv	AT christianklose flexibilityofaeukaryoticlipidomeinsightsfromyeastlipidomics AT michalasurma flexibilityofaeukaryoticlipidomeinsightsfromyeastlipidomics AT mathiasjgerl flexibilityofaeukaryoticlipidomeinsightsfromyeastlipidomics AT felixmeyenhofer flexibilityofaeukaryoticlipidomeinsightsfromyeastlipidomics AT andrejshevchenko flexibilityofaeukaryoticlipidomeinsightsfromyeastlipidomics AT kaisimons flexibilityofaeukaryoticlipidomeinsightsfromyeastlipidomics
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Flexibility of a eukaryotic lipidome--insights from yeast lipidomics.

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