Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research

Abstract There is emerging evidence that microRNAs (miRNAs) dysregulation is involved in the genesis and the progression of Prostate Cancer (PCa), thus potentially increasing their use in urological clinical practice. This is the first pilot study which utilizes Illumina Deep Sequencing to examine t...

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Autores principales:	Gabriella Guelfi, Giovanni Cochetti, Valentina Stefanetti, Danilo Zampini, Silvana Diverio, Andrea Boni, Ettore Mearini
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed8ff32997bd4cbd83124e080b3d91c3
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spelling	oai:doaj.org-article:ed8ff32997bd4cbd83124e080b3d91c32021-12-02T16:08:13ZNext Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research10.1038/s41598-018-24236-y2045-2322https://doaj.org/article/ed8ff32997bd4cbd83124e080b3d91c32018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24236-yhttps://doaj.org/toc/2045-2322Abstract There is emerging evidence that microRNAs (miRNAs) dysregulation is involved in the genesis and the progression of Prostate Cancer (PCa), thus potentially increasing their use in urological clinical practice. This is the first pilot study which utilizes Illumina Deep Sequencing to examine the entire miRNAs spectrum existent in urine exfoliated prostate cells (UEPCs) of PCa patients. A total of 11 male patients with histological diagnosis of PCa were enrolled in the present study. First-catch urine (30 mL) was collected following a prostate massage. Total RNA was extracted from urine and sequenced using an HiSeq2500 System (Illumina). QPCR assay was used to validate the highest NGS results in PCA patients and in age-matched, caucasian men. Remarkably, PCA let-7 family was down-regulated (P < 0.01), compared to the controls. The results of our study support the notion of a relatively high diagnostic value of miRNA family for PCa detection, especially in the let-7 family. The present research confirmed the potential use of miRNAs as non-invasive biomarkers in the diagnosis of PCa, potentially reducing the invasiveness of actual clinical strategy.Gabriella GuelfiGiovanni CochettiValentina StefanettiDanilo ZampiniSilvana DiverioAndrea BoniEttore MeariniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gabriella Guelfi Giovanni Cochetti Valentina Stefanetti Danilo Zampini Silvana Diverio Andrea Boni Ettore Mearini Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research
description	Abstract There is emerging evidence that microRNAs (miRNAs) dysregulation is involved in the genesis and the progression of Prostate Cancer (PCa), thus potentially increasing their use in urological clinical practice. This is the first pilot study which utilizes Illumina Deep Sequencing to examine the entire miRNAs spectrum existent in urine exfoliated prostate cells (UEPCs) of PCa patients. A total of 11 male patients with histological diagnosis of PCa were enrolled in the present study. First-catch urine (30 mL) was collected following a prostate massage. Total RNA was extracted from urine and sequenced using an HiSeq2500 System (Illumina). QPCR assay was used to validate the highest NGS results in PCA patients and in age-matched, caucasian men. Remarkably, PCA let-7 family was down-regulated (P < 0.01), compared to the controls. The results of our study support the notion of a relatively high diagnostic value of miRNA family for PCa detection, especially in the let-7 family. The present research confirmed the potential use of miRNAs as non-invasive biomarkers in the diagnosis of PCa, potentially reducing the invasiveness of actual clinical strategy.
format	article
author	Gabriella Guelfi Giovanni Cochetti Valentina Stefanetti Danilo Zampini Silvana Diverio Andrea Boni Ettore Mearini
author_facet	Gabriella Guelfi Giovanni Cochetti Valentina Stefanetti Danilo Zampini Silvana Diverio Andrea Boni Ettore Mearini
author_sort	Gabriella Guelfi
title	Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research
title_short	Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research
title_full	Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research
title_fullStr	Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research
title_full_unstemmed	Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research
title_sort	next generation sequencing of urine exfoliated cells: an approach of prostate cancer micrornas research
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ed8ff32997bd4cbd83124e080b3d91c3
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Next Generation Sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research

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