DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.

The development of efficient vaccines against COVID-19 is an emergent need for global public health. The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a major target for the COVID-19 vaccine. To quickly respond to the outbreak of the SARS-CoV-2 pandemic, a nucleic...

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Autores principales:	Kit Man Chai, Tsai-Teng Tzeng, Kuan-Yin Shen, Hung-Chun Liao, Jhe-Jhih Lin, Mei-Yu Chen, Guann-Yi Yu, Horng-Yunn Dou, Ching-Len Liao, Hsin-Wei Chen, Shih-Jen Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/ed966694d2fb48dfa78c64314db836a2
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spelling	oai:doaj.org-article:ed966694d2fb48dfa78c64314db836a22021-12-02T20:24:18ZDNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.1935-27271935-273510.1371/journal.pntd.0009374https://doaj.org/article/ed966694d2fb48dfa78c64314db836a22021-05-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009374https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735The development of efficient vaccines against COVID-19 is an emergent need for global public health. The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a major target for the COVID-19 vaccine. To quickly respond to the outbreak of the SARS-CoV-2 pandemic, a nucleic acid-based vaccine is a novel option, beyond the traditional inactivated virus vaccine or recombinant protein vaccine. Here, we report a DNA vaccine containing the spike gene for delivery via electroporation. The spike genes of SARS-CoV and SARS-CoV-2 were codon optimized for mammalian cell expression and then cloned into mammalian cell expression vectors, called pSARS-S and pSARS2-S, respectively. Spike protein expression was confirmed by immunoblotting after transient expression in HEK293T cells. After immunization, sera were collected for antigen-specific antibody and neutralizing antibody titer analyses. We found that both pSARS-S and pSARS2-S immunization induced similar levels of antibodies against S2 of SARS-CoV-2. In contrast, only pSARS2-S immunization induced antibodies against the receptor-binding domain of SARS-CoV-2. We further found that pSARS2-S immunization, but not pSARS-S immunization, could induce very high titers of neutralizing antibodies against SARS-CoV-2. We further analyzed SARS-CoV-2 S protein-specific T cell responses and found that the immune responses were biased toward Th1. Importantly, pSARS2-S immunization in hamsters could induce protective immunity against SARS-CoV-2 challenge in vivo. These data suggest that DNA vaccination could be a promising approach for protecting against COVID-19.Kit Man ChaiTsai-Teng TzengKuan-Yin ShenHung-Chun LiaoJhe-Jhih LinMei-Yu ChenGuann-Yi YuHorng-Yunn DouChing-Len LiaoHsin-Wei ChenShih-Jen LiuPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 5, p e0009374 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kit Man Chai Tsai-Teng Tzeng Kuan-Yin Shen Hung-Chun Liao Jhe-Jhih Lin Mei-Yu Chen Guann-Yi Yu Horng-Yunn Dou Ching-Len Liao Hsin-Wei Chen Shih-Jen Liu DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.
description	The development of efficient vaccines against COVID-19 is an emergent need for global public health. The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a major target for the COVID-19 vaccine. To quickly respond to the outbreak of the SARS-CoV-2 pandemic, a nucleic acid-based vaccine is a novel option, beyond the traditional inactivated virus vaccine or recombinant protein vaccine. Here, we report a DNA vaccine containing the spike gene for delivery via electroporation. The spike genes of SARS-CoV and SARS-CoV-2 were codon optimized for mammalian cell expression and then cloned into mammalian cell expression vectors, called pSARS-S and pSARS2-S, respectively. Spike protein expression was confirmed by immunoblotting after transient expression in HEK293T cells. After immunization, sera were collected for antigen-specific antibody and neutralizing antibody titer analyses. We found that both pSARS-S and pSARS2-S immunization induced similar levels of antibodies against S2 of SARS-CoV-2. In contrast, only pSARS2-S immunization induced antibodies against the receptor-binding domain of SARS-CoV-2. We further found that pSARS2-S immunization, but not pSARS-S immunization, could induce very high titers of neutralizing antibodies against SARS-CoV-2. We further analyzed SARS-CoV-2 S protein-specific T cell responses and found that the immune responses were biased toward Th1. Importantly, pSARS2-S immunization in hamsters could induce protective immunity against SARS-CoV-2 challenge in vivo. These data suggest that DNA vaccination could be a promising approach for protecting against COVID-19.
format	article
author	Kit Man Chai Tsai-Teng Tzeng Kuan-Yin Shen Hung-Chun Liao Jhe-Jhih Lin Mei-Yu Chen Guann-Yi Yu Horng-Yunn Dou Ching-Len Liao Hsin-Wei Chen Shih-Jen Liu
author_facet	Kit Man Chai Tsai-Teng Tzeng Kuan-Yin Shen Hung-Chun Liao Jhe-Jhih Lin Mei-Yu Chen Guann-Yi Yu Horng-Yunn Dou Ching-Len Liao Hsin-Wei Chen Shih-Jen Liu
author_sort	Kit Man Chai
title	DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.
title_short	DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.
title_full	DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.
title_fullStr	DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.
title_full_unstemmed	DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.
title_sort	dna vaccination induced protective immunity against sars cov-2 infection in hamsterss.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ed966694d2fb48dfa78c64314db836a2
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DNA vaccination induced protective immunity against SARS CoV-2 infection in hamsterss.

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