Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence

Abstract Exophiala dermatitidis causes chromoblastomycosis, phaeohyphomycosis and fatal infections of the central nervous system of patients with Asian background. It is also found in respiratory secretions from cystic fibrosis (CF) patients. In this study a variety of E. dermatitidis strains (isola...

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Autores principales:	Maike Olsowski, Frederike Hoffmann, Andrea Hain, Lisa Kirchhoff, Dirk Theegarten, Daniel Todt, Eike Steinmann, Jan Buer, Peter-Michael Rath, Joerg Steinmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed9bb42ede9848de978771796193e1af
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spelling	oai:doaj.org-article:ed9bb42ede9848de978771796193e1af2021-12-02T15:09:11ZExophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence10.1038/s41598-018-30909-52045-2322https://doaj.org/article/ed9bb42ede9848de978771796193e1af2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30909-5https://doaj.org/toc/2045-2322Abstract Exophiala dermatitidis causes chromoblastomycosis, phaeohyphomycosis and fatal infections of the central nervous system of patients with Asian background. It is also found in respiratory secretions from cystic fibrosis (CF) patients. In this study a variety of E. dermatitidis strains (isolates from Asia, environmental and CF) were characterized in their pathogenicity by survival analyzes using two different invertebrate host organisms, Caenorhabditis elegans and Galleria mellonella. Furthermore, the morphological development of hyphal formation was analyzed. E. dermatitidis exhibited pathogenicity in C. elegans. The virulence varied in a strain-dependent manner, but the nematodes were a limited model to study hyphal formation. Analysis of a melanin-deficient mutant (Mel-3) indicates that melanin plays a role during virulence processes in C. elegans. The strains isolated from Asian patients exhibited significantly higher virulence in G. mellonella compared to strains from other sources. Histological analyzes also revealed a higher potential of invasive hyphal growth in strains isolated from Asian patients. Interestingly, no significant difference was found in virulence between the Mel-3 mutant and their wild type counterpart during infection in G. mellonella. In conclusion, invasive hyphal formation of E. dermatitidis was associated with increased virulence. This work is the basis for future studies concerning E. dermatitidis virulence.Maike OlsowskiFrederike HoffmannAndrea HainLisa KirchhoffDirk TheegartenDaniel TodtEike SteinmannJan BuerPeter-Michael RathJoerg SteinmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maike Olsowski Frederike Hoffmann Andrea Hain Lisa Kirchhoff Dirk Theegarten Daniel Todt Eike Steinmann Jan Buer Peter-Michael Rath Joerg Steinmann Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence
description	Abstract Exophiala dermatitidis causes chromoblastomycosis, phaeohyphomycosis and fatal infections of the central nervous system of patients with Asian background. It is also found in respiratory secretions from cystic fibrosis (CF) patients. In this study a variety of E. dermatitidis strains (isolates from Asia, environmental and CF) were characterized in their pathogenicity by survival analyzes using two different invertebrate host organisms, Caenorhabditis elegans and Galleria mellonella. Furthermore, the morphological development of hyphal formation was analyzed. E. dermatitidis exhibited pathogenicity in C. elegans. The virulence varied in a strain-dependent manner, but the nematodes were a limited model to study hyphal formation. Analysis of a melanin-deficient mutant (Mel-3) indicates that melanin plays a role during virulence processes in C. elegans. The strains isolated from Asian patients exhibited significantly higher virulence in G. mellonella compared to strains from other sources. Histological analyzes also revealed a higher potential of invasive hyphal growth in strains isolated from Asian patients. Interestingly, no significant difference was found in virulence between the Mel-3 mutant and their wild type counterpart during infection in G. mellonella. In conclusion, invasive hyphal formation of E. dermatitidis was associated with increased virulence. This work is the basis for future studies concerning E. dermatitidis virulence.
format	article
author	Maike Olsowski Frederike Hoffmann Andrea Hain Lisa Kirchhoff Dirk Theegarten Daniel Todt Eike Steinmann Jan Buer Peter-Michael Rath Joerg Steinmann
author_facet	Maike Olsowski Frederike Hoffmann Andrea Hain Lisa Kirchhoff Dirk Theegarten Daniel Todt Eike Steinmann Jan Buer Peter-Michael Rath Joerg Steinmann
author_sort	Maike Olsowski
title	Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence
title_short	Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence
title_full	Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence
title_fullStr	Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence
title_full_unstemmed	Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence
title_sort	exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (caenorhabditis elegans and galleria mellonella) to determine virulence
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/ed9bb42ede9848de978771796193e1af
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Exophiala dermatitidis isolates from various sources: using alternative invertebrate host organisms (Caenorhabditis elegans and Galleria mellonella) to determine virulence

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