Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system

Abstract Bacterial culture and drug susceptibility testing are used to identify pathogen infections. Nevertheless, the process requires several days from collection to the identification of bacterial species and drug-resistance patterns. The digital PCR system is a rapidly developing quantitative de...

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Autores principales:	Tiantian Zhang, Zhiqiang Niu, Feng Wu, Zongkun Chen, Jun Xu, Kewei Jiang, Zhiyong Lai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ed9e2a0bf4b349cba6458268688acc72
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spelling	oai:doaj.org-article:ed9e2a0bf4b349cba6458268688acc722021-12-02T18:27:48ZQualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system10.1038/s41598-021-87824-52045-2322https://doaj.org/article/ed9e2a0bf4b349cba6458268688acc722021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87824-5https://doaj.org/toc/2045-2322Abstract Bacterial culture and drug susceptibility testing are used to identify pathogen infections. Nevertheless, the process requires several days from collection to the identification of bacterial species and drug-resistance patterns. The digital PCR system is a rapidly developing quantitative detection technology widely applied to molecular diagnosis, including copy number variations, single nucleotide variant analysis, cancer biomarker discovery, and pathogen identification. This study aimed to use a droplet digital PCR system to identify bacteria in blood samples and explore its ability to identify pathogen in bacteremia. Then, we designed primers and probes of SWG-9 and COA gene for E. coli and S. aureus to identify in blood samples with the ddPCR system. The system had demonstrated extremely high detection accuracy in blood samples, and the detection rate of E. coli was 13.1–21.4%, and that of S. aureus was 50–88.3%. Finally, blood samples containing both E. coli and S. aureus were tested to evaluate further the accuracy and applicability of this method, indicating the detection rates range from 18.1% to 97%. The ddPCR system is highly promising as a qualitatively and quantitatively screening method for rapidly detecting pathogen.Tiantian ZhangZhiqiang NiuFeng WuZongkun ChenJun XuKewei JiangZhiyong LaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tiantian Zhang Zhiqiang Niu Feng Wu Zongkun Chen Jun Xu Kewei Jiang Zhiyong Lai Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system
description	Abstract Bacterial culture and drug susceptibility testing are used to identify pathogen infections. Nevertheless, the process requires several days from collection to the identification of bacterial species and drug-resistance patterns. The digital PCR system is a rapidly developing quantitative detection technology widely applied to molecular diagnosis, including copy number variations, single nucleotide variant analysis, cancer biomarker discovery, and pathogen identification. This study aimed to use a droplet digital PCR system to identify bacteria in blood samples and explore its ability to identify pathogen in bacteremia. Then, we designed primers and probes of SWG-9 and COA gene for E. coli and S. aureus to identify in blood samples with the ddPCR system. The system had demonstrated extremely high detection accuracy in blood samples, and the detection rate of E. coli was 13.1–21.4%, and that of S. aureus was 50–88.3%. Finally, blood samples containing both E. coli and S. aureus were tested to evaluate further the accuracy and applicability of this method, indicating the detection rates range from 18.1% to 97%. The ddPCR system is highly promising as a qualitatively and quantitatively screening method for rapidly detecting pathogen.
format	article
author	Tiantian Zhang Zhiqiang Niu Feng Wu Zongkun Chen Jun Xu Kewei Jiang Zhiyong Lai
author_facet	Tiantian Zhang Zhiqiang Niu Feng Wu Zongkun Chen Jun Xu Kewei Jiang Zhiyong Lai
author_sort	Tiantian Zhang
title	Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system
title_short	Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system
title_full	Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system
title_fullStr	Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system
title_full_unstemmed	Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system
title_sort	qualitative and quantitative detection of surgical pathogenic microorganisms escherichia coli and staphylococcus aureus based on ddpcr system
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ed9e2a0bf4b349cba6458268688acc72
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Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system

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