Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.

<h4>Background</h4>Mesenchymal stem cells (MSCs) participate in the regulation of inflammation and innate immunity, for example by responding to pathogen-derived signals and by regulating the function of innate immune cells. MSCs from the bone-marrow and peripheral tissues share common b...

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Autores principales:	Sven Brandau, Mark Jakob, Kirsten Bruderek, Friedrich Bootz, Bernd Giebel, Stefan Radtke, Katharina Mauel, Marcus Jäger, Stefanie B Flohé, Stephan Lang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/edaabd2e597a4f769767c1c4279d9ba1
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spelling	oai:doaj.org-article:edaabd2e597a4f769767c1c4279d9ba12021-11-25T06:00:00ZMesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.1932-620310.1371/journal.pone.0106903https://doaj.org/article/edaabd2e597a4f769767c1c4279d9ba12014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0106903https://doaj.org/toc/1932-6203<h4>Background</h4>Mesenchymal stem cells (MSCs) participate in the regulation of inflammation and innate immunity, for example by responding to pathogen-derived signals and by regulating the function of innate immune cells. MSCs from the bone-marrow and peripheral tissues share common basic cell-biological functions. However, it is unknown whether these MSCs exhibit different responses to microbial challenge and whether this response subsequently modulates the regulation of inflammatory cells by MSCs.<h4>Methodology/principal findings</h4>We isolated MSCs from human bone-marrow (bmMSCs) and human salivary gland (pgMSCs). Expression levels of TLR4 and LPS-responsive molecules were determined by flow cytometry and quantitative PCR. Cytokine release was determined by ELISA. The effect of supernatants from unstimulated and LPS-stimulated MSCs on recruitment, cytokine secretion, bacterial clearance and oxidative burst of polymorphonuclear neutrophil granulocytes (PMN) was tested in vitro. Despite minor quantitative differences, bmMSCs and pgMSCs showed a similar cell biological response to bacterial endotoxin. Both types of MSCs augmented anti-microbial functions of PMNs. LPS stimulation, particularly of bmMSCs, further augmented MSC-mediated activation of PMN [corrected].<h4>Conclusions/significance</h4>This study suggests that MSCs may contribute to the resolution of infection and inflammation by promoting the anti-microbial activity of PMNs. This property is exerted by MSCs derived from both the bone-marrow and peripheral glandular tissue.Sven BrandauMark JakobKirsten BruderekFriedrich BootzBernd GiebelStefan RadtkeKatharina MauelMarcus JägerStefanie B FlohéStephan LangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106903 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sven Brandau Mark Jakob Kirsten Bruderek Friedrich Bootz Bernd Giebel Stefan Radtke Katharina Mauel Marcus Jäger Stefanie B Flohé Stephan Lang Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.
description	<h4>Background</h4>Mesenchymal stem cells (MSCs) participate in the regulation of inflammation and innate immunity, for example by responding to pathogen-derived signals and by regulating the function of innate immune cells. MSCs from the bone-marrow and peripheral tissues share common basic cell-biological functions. However, it is unknown whether these MSCs exhibit different responses to microbial challenge and whether this response subsequently modulates the regulation of inflammatory cells by MSCs.<h4>Methodology/principal findings</h4>We isolated MSCs from human bone-marrow (bmMSCs) and human salivary gland (pgMSCs). Expression levels of TLR4 and LPS-responsive molecules were determined by flow cytometry and quantitative PCR. Cytokine release was determined by ELISA. The effect of supernatants from unstimulated and LPS-stimulated MSCs on recruitment, cytokine secretion, bacterial clearance and oxidative burst of polymorphonuclear neutrophil granulocytes (PMN) was tested in vitro. Despite minor quantitative differences, bmMSCs and pgMSCs showed a similar cell biological response to bacterial endotoxin. Both types of MSCs augmented anti-microbial functions of PMNs. LPS stimulation, particularly of bmMSCs, further augmented MSC-mediated activation of PMN [corrected].<h4>Conclusions/significance</h4>This study suggests that MSCs may contribute to the resolution of infection and inflammation by promoting the anti-microbial activity of PMNs. This property is exerted by MSCs derived from both the bone-marrow and peripheral glandular tissue.
format	article
author	Sven Brandau Mark Jakob Kirsten Bruderek Friedrich Bootz Bernd Giebel Stefan Radtke Katharina Mauel Marcus Jäger Stefanie B Flohé Stephan Lang
author_facet	Sven Brandau Mark Jakob Kirsten Bruderek Friedrich Bootz Bernd Giebel Stefan Radtke Katharina Mauel Marcus Jäger Stefanie B Flohé Stephan Lang
author_sort	Sven Brandau
title	Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.
title_short	Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.
title_full	Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.
title_fullStr	Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.
title_full_unstemmed	Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.
title_sort	mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/edaabd2e597a4f769767c1c4279d9ba1
work_keys_str_mv	AT svenbrandau mesenchymalstemcellsaugmenttheantibacterialactivityofneutrophilgranulocytes AT markjakob mesenchymalstemcellsaugmenttheantibacterialactivityofneutrophilgranulocytes AT kirstenbruderek mesenchymalstemcellsaugmenttheantibacterialactivityofneutrophilgranulocytes AT friedrichbootz mesenchymalstemcellsaugmenttheantibacterialactivityofneutrophilgranulocytes AT berndgiebel mesenchymalstemcellsaugmenttheantibacterialactivityofneutrophilgranulocytes AT stefanradtke mesenchymalstemcellsaugmenttheantibacterialactivityofneutrophilgranulocytes AT katharinamauel mesenchymalstemcellsaugmenttheantibacterialactivityofneutrophilgranulocytes AT marcusjager mesenchymalstemcellsaugmenttheantibacterialactivityofneutrophilgranulocytes AT stefaniebflohe mesenchymalstemcellsaugmenttheantibacterialactivityofneutrophilgranulocytes AT stephanlang mesenchymalstemcellsaugmenttheantibacterialactivityofneutrophilgranulocytes
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Mesenchymal stem cells augment the anti-bacterial activity of neutrophil granulocytes.

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