3D Liquid Marble Microbioreactors Support <i>In Vitro</i> Maturation of Prepubertal Ovine Oocytes and Affect Expression of Oocyte-Specific Factors

In vitro oocyte maturation (IVM) is a well-established technique. Despite the high IVM rates obtained in most mammalian species, the developmental competence of IVM oocytes is suboptimal. The aim of this work was to evaluate the potential beneficial effects of a liquid marble microbioreactor (LM) as...

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Autores principales:	Daniela Bebbere, Stefano Mario Nieddu, Federica Ariu, Davide Piras, Sergio Ledda
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	low developmental competence gene expression 3D in vitro culture Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/edb1ff66e8994df2854e245369ef0617
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spelling	oai:doaj.org-article:edb1ff66e8994df2854e245369ef06172021-11-25T16:47:00Z3D Liquid Marble Microbioreactors Support <i>In Vitro</i> Maturation of Prepubertal Ovine Oocytes and Affect Expression of Oocyte-Specific Factors10.3390/biology101111012079-7737https://doaj.org/article/edb1ff66e8994df2854e245369ef06172021-10-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1101https://doaj.org/toc/2079-7737In vitro oocyte maturation (IVM) is a well-established technique. Despite the high IVM rates obtained in most mammalian species, the developmental competence of IVM oocytes is suboptimal. The aim of this work was to evaluate the potential beneficial effects of a liquid marble microbioreactor (LM) as a 3D culture system to mature in vitro prepubertal ovine oocytes, as models of oocytes with intrinsic low competence. Cumulus–oocyte complexes of prepubertal sheep ovaries were in vitro matured in a LM system with hydrophobic fumed-silica-nanoparticles (LM group) or in standard conditions (4W control group). We evaluated: (a) maturation and (b) developmental rates following in vitro fertilization (IVF) and embryo culture; (c) expression of a panel of genes. LM and 4W groups showed similar IVM and IVF rates, while in vitro development to blastocyst stage approached significance (4W: 14.1% vs. LM: 28.3%; <i>p</i> = 0.066). The expression of <i>GDF9</i>, of enzymes involved in DNA methylation reprogramming and of the subcortical maternal complex was affected by the IVM system, while no difference was observed in terms of cell-stress-response. LM microbioreactors provide a suitable microenvironment to induce prepubertal sheep oocyte IVM and should be considered to enhance the developmental competence of oocytes with reduced potential also in other species, including humans.Daniela BebbereStefano Mario NiedduFederica AriuDavide PirasSergio LeddaMDPI AGarticlelow developmental competencegene expression3D in vitro cultureBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1101, p 1101 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	low developmental competence gene expression 3D in vitro culture Biology (General) QH301-705.5
spellingShingle	low developmental competence gene expression 3D in vitro culture Biology (General) QH301-705.5 Daniela Bebbere Stefano Mario Nieddu Federica Ariu Davide Piras Sergio Ledda 3D Liquid Marble Microbioreactors Support <i>In Vitro</i> Maturation of Prepubertal Ovine Oocytes and Affect Expression of Oocyte-Specific Factors
description	In vitro oocyte maturation (IVM) is a well-established technique. Despite the high IVM rates obtained in most mammalian species, the developmental competence of IVM oocytes is suboptimal. The aim of this work was to evaluate the potential beneficial effects of a liquid marble microbioreactor (LM) as a 3D culture system to mature in vitro prepubertal ovine oocytes, as models of oocytes with intrinsic low competence. Cumulus–oocyte complexes of prepubertal sheep ovaries were in vitro matured in a LM system with hydrophobic fumed-silica-nanoparticles (LM group) or in standard conditions (4W control group). We evaluated: (a) maturation and (b) developmental rates following in vitro fertilization (IVF) and embryo culture; (c) expression of a panel of genes. LM and 4W groups showed similar IVM and IVF rates, while in vitro development to blastocyst stage approached significance (4W: 14.1% vs. LM: 28.3%; <i>p</i> = 0.066). The expression of <i>GDF9</i>, of enzymes involved in DNA methylation reprogramming and of the subcortical maternal complex was affected by the IVM system, while no difference was observed in terms of cell-stress-response. LM microbioreactors provide a suitable microenvironment to induce prepubertal sheep oocyte IVM and should be considered to enhance the developmental competence of oocytes with reduced potential also in other species, including humans.
format	article
author	Daniela Bebbere Stefano Mario Nieddu Federica Ariu Davide Piras Sergio Ledda
author_facet	Daniela Bebbere Stefano Mario Nieddu Federica Ariu Davide Piras Sergio Ledda
author_sort	Daniela Bebbere
title	3D Liquid Marble Microbioreactors Support <i>In Vitro</i> Maturation of Prepubertal Ovine Oocytes and Affect Expression of Oocyte-Specific Factors
title_short	3D Liquid Marble Microbioreactors Support <i>In Vitro</i> Maturation of Prepubertal Ovine Oocytes and Affect Expression of Oocyte-Specific Factors
title_full	3D Liquid Marble Microbioreactors Support <i>In Vitro</i> Maturation of Prepubertal Ovine Oocytes and Affect Expression of Oocyte-Specific Factors
title_fullStr	3D Liquid Marble Microbioreactors Support <i>In Vitro</i> Maturation of Prepubertal Ovine Oocytes and Affect Expression of Oocyte-Specific Factors
title_full_unstemmed	3D Liquid Marble Microbioreactors Support <i>In Vitro</i> Maturation of Prepubertal Ovine Oocytes and Affect Expression of Oocyte-Specific Factors
title_sort	3d liquid marble microbioreactors support <i>in vitro</i> maturation of prepubertal ovine oocytes and affect expression of oocyte-specific factors
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/edb1ff66e8994df2854e245369ef0617
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3D Liquid Marble Microbioreactors Support <i>In Vitro</i> Maturation of Prepubertal Ovine Oocytes and Affect Expression of Oocyte-Specific Factors

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