Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti

Abstract. Prayogo NA, Wijayanti GE, Sulistyo I. Sukardi P. 2016. Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti. Biodiversitas 17: xxx. Gonadotropin-releasing hormone (GnRH) is synthesized in the brain and acts in the anterior pit...

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Autores principales:	N. A. PRAYOGO, G. E. WIJAYANTI, I. SULISTYO, P. SUKARDI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2016
Materias:	cgnrh-ii, type 1 and 2, real time-pcr, amino acid Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/edb929ef7d1549f8a05fcdc72e3c8e8b
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spelling	oai:doaj.org-article:edb929ef7d1549f8a05fcdc72e3c8e8b2021-11-14T02:27:09ZIdentification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti1412-033X2085-472210.13057/biodiv/d170222https://doaj.org/article/edb929ef7d1549f8a05fcdc72e3c8e8b2016-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/326https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Prayogo NA, Wijayanti GE, Sulistyo I. Sukardi P. 2016. Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti. Biodiversitas 17: xxx. Gonadotropin-releasing hormone (GnRH) is synthesized in the brain and acts in the anterior pituitary to stimulate the release of gonadotropins in fishes as well as in other vertebrates. Genomic DNAs and cDNAs of two chicken-type GnRH-II genes of hard-lipped barb, namely cGnRH-II type 1 and type 2, were cloned. The length of cloned genomic DNA of cGnRH-II type 1 was 580 bp and cDNA was 206 bp. The length of cloned genomic DNA of cGnRH-II type 2 was 570 bp and cDNA was 196 bp. The cGnRH-II type 1 and type 2 cDNAs encode precursors of 68 and 63 amino acids, respectively. Those precursors consist of a signal peptide, cGnRH-II decapeptide and a GnRH-associated peptide (GAP) linked by a Gly-Lys-Arg proteolytic site. Using quantitative Real Time-PCR, expression levels of these two cGnRH-II genes were detected in the brain, liver and gonad of hard-lipped barb. Expression of the GnRH-II type 1 gene was found only in the brain and liver, on the other hand, expression of the cGnRH-II type 2 gene was found in the gonad, in addition to the brain and liver. The expression of the cGnRH-II genes outside the brain suggested that cGnRH-II might act as an autocrine or paracrine regulator.N. A. PRAYOGOG. E. WIJAYANTII. SULISTYOP. SUKARDIMBI & UNS Soloarticlecgnrh-ii, type 1 and 2, real time-pcr, amino acidBiology (General)QH301-705.5ENBiodiversitas, Vol 17, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	cgnrh-ii, type 1 and 2, real time-pcr, amino acid Biology (General) QH301-705.5
spellingShingle	cgnrh-ii, type 1 and 2, real time-pcr, amino acid Biology (General) QH301-705.5 N. A. PRAYOGO G. E. WIJAYANTI I. SULISTYO P. SUKARDI Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti
description	Abstract. Prayogo NA, Wijayanti GE, Sulistyo I. Sukardi P. 2016. Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti. Biodiversitas 17: xxx. Gonadotropin-releasing hormone (GnRH) is synthesized in the brain and acts in the anterior pituitary to stimulate the release of gonadotropins in fishes as well as in other vertebrates. Genomic DNAs and cDNAs of two chicken-type GnRH-II genes of hard-lipped barb, namely cGnRH-II type 1 and type 2, were cloned. The length of cloned genomic DNA of cGnRH-II type 1 was 580 bp and cDNA was 206 bp. The length of cloned genomic DNA of cGnRH-II type 2 was 570 bp and cDNA was 196 bp. The cGnRH-II type 1 and type 2 cDNAs encode precursors of 68 and 63 amino acids, respectively. Those precursors consist of a signal peptide, cGnRH-II decapeptide and a GnRH-associated peptide (GAP) linked by a Gly-Lys-Arg proteolytic site. Using quantitative Real Time-PCR, expression levels of these two cGnRH-II genes were detected in the brain, liver and gonad of hard-lipped barb. Expression of the GnRH-II type 1 gene was found only in the brain and liver, on the other hand, expression of the cGnRH-II type 2 gene was found in the gonad, in addition to the brain and liver. The expression of the cGnRH-II genes outside the brain suggested that cGnRH-II might act as an autocrine or paracrine regulator.
format	article
author	N. A. PRAYOGO G. E. WIJAYANTI I. SULISTYO P. SUKARDI
author_facet	N. A. PRAYOGO G. E. WIJAYANTI I. SULISTYO P. SUKARDI
author_sort	N. A. PRAYOGO
title	Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti
title_short	Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti
title_full	Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti
title_fullStr	Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti
title_full_unstemmed	Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti
title_sort	identification and expression of two types of chicken gnrh-ii genes in mature hard-lipped barb, osteochilus hasselti
publisher	MBI & UNS Solo
publishDate	2016
url	https://doaj.org/article/edb929ef7d1549f8a05fcdc72e3c8e8b
work_keys_str_mv	AT naprayogo identificationandexpressionoftwotypesofchickengnrhiigenesinmaturehardlippedbarbosteochilushasselti AT gewijayanti identificationandexpressionoftwotypesofchickengnrhiigenesinmaturehardlippedbarbosteochilushasselti AT isulistyo identificationandexpressionoftwotypesofchickengnrhiigenesinmaturehardlippedbarbosteochilushasselti AT psukardi identificationandexpressionoftwotypesofchickengnrhiigenesinmaturehardlippedbarbosteochilushasselti
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Identification and expression of two types of chicken GnRH-II genes in mature hard-lipped barb, Osteochilus hasselti

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