Proteins associated with SF3a60 in T. brucei.

Trypanosoma brucei relies on Spliced leader trans splicing to generate functional messenger RNAs. Trans splicing joins the specialized SL exon from the SL RNA to pre-mRNAs and is mediated by the trans-spliceosome, which is made up of small nuclear ribonucleoprotein particles and non-snRNP factors. A...

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Autores principales:	Benson Nyambega, Claudia Helbig, Daniel K Masiga, Christine Clayton, Mariano J Levin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/edbb0c163e064887a91b3e1e49ee53c4
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spelling	oai:doaj.org-article:edbb0c163e064887a91b3e1e49ee53c42021-11-18T08:27:04ZProteins associated with SF3a60 in T. brucei.1932-620310.1371/journal.pone.0091956https://doaj.org/article/edbb0c163e064887a91b3e1e49ee53c42014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24651488/?tool=EBIhttps://doaj.org/toc/1932-6203Trypanosoma brucei relies on Spliced leader trans splicing to generate functional messenger RNAs. Trans splicing joins the specialized SL exon from the SL RNA to pre-mRNAs and is mediated by the trans-spliceosome, which is made up of small nuclear ribonucleoprotein particles and non-snRNP factors. Although the trans spliceosome is essential for trypanosomatid gene expression, not all spliceosomal protein factors are known and of these, only a few are completely characterized. In this study, we have characterized the trypanosome Splicing Factor, SF3a60, the only currently annotated SF3a component. As expected, epitope-tagged SF3a60 localizes in the trypanosome nucleus. SF3a60 is essential for cell viability but its depletion seem to have no detectable effect on trans-splicing. In addition, we used SF3a60 as bait in a Yeast-2-hybrid system screen and identified its interacting protein factors. The interactions with SF3a120, SF3a66 and SAP130 were confirmed by tandem affinity purification and mass spectrometry.Benson NyambegaClaudia HelbigDaniel K MasigaChristine ClaytonMariano J LevinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91956 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benson Nyambega Claudia Helbig Daniel K Masiga Christine Clayton Mariano J Levin Proteins associated with SF3a60 in T. brucei.
description	Trypanosoma brucei relies on Spliced leader trans splicing to generate functional messenger RNAs. Trans splicing joins the specialized SL exon from the SL RNA to pre-mRNAs and is mediated by the trans-spliceosome, which is made up of small nuclear ribonucleoprotein particles and non-snRNP factors. Although the trans spliceosome is essential for trypanosomatid gene expression, not all spliceosomal protein factors are known and of these, only a few are completely characterized. In this study, we have characterized the trypanosome Splicing Factor, SF3a60, the only currently annotated SF3a component. As expected, epitope-tagged SF3a60 localizes in the trypanosome nucleus. SF3a60 is essential for cell viability but its depletion seem to have no detectable effect on trans-splicing. In addition, we used SF3a60 as bait in a Yeast-2-hybrid system screen and identified its interacting protein factors. The interactions with SF3a120, SF3a66 and SAP130 were confirmed by tandem affinity purification and mass spectrometry.
format	article
author	Benson Nyambega Claudia Helbig Daniel K Masiga Christine Clayton Mariano J Levin
author_facet	Benson Nyambega Claudia Helbig Daniel K Masiga Christine Clayton Mariano J Levin
author_sort	Benson Nyambega
title	Proteins associated with SF3a60 in T. brucei.
title_short	Proteins associated with SF3a60 in T. brucei.
title_full	Proteins associated with SF3a60 in T. brucei.
title_fullStr	Proteins associated with SF3a60 in T. brucei.
title_full_unstemmed	Proteins associated with SF3a60 in T. brucei.
title_sort	proteins associated with sf3a60 in t. brucei.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/edbb0c163e064887a91b3e1e49ee53c4
work_keys_str_mv	AT bensonnyambega proteinsassociatedwithsf3a60intbrucei AT claudiahelbig proteinsassociatedwithsf3a60intbrucei AT danielkmasiga proteinsassociatedwithsf3a60intbrucei AT christineclayton proteinsassociatedwithsf3a60intbrucei AT marianojlevin proteinsassociatedwithsf3a60intbrucei
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Proteins associated with SF3a60 in T. brucei.

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