MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing

Abstract MicroRNA (miR)-132 has been identified as a top up-regulated miRNA during skin wound healing and its inhibition impairs wound repair. In a human in vivo surgical wound model, we showed that miR-132 was induced in epidermal as well as in dermal wound–edge compartments during healing. Moreove...

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Autores principales: Xi Li, Dongqing Li, Jakob D. Wikstrom, Andor Pivarcsi, Enikö Sonkoly, Mona Ståhle, Ning Xu Landén
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/edc64160f4cc426d8b6f4e11e8a1ef43
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spelling oai:doaj.org-article:edc64160f4cc426d8b6f4e11e8a1ef432021-12-02T15:06:03ZMicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing10.1038/s41598-017-07513-02045-2322https://doaj.org/article/edc64160f4cc426d8b6f4e11e8a1ef432017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07513-0https://doaj.org/toc/2045-2322Abstract MicroRNA (miR)-132 has been identified as a top up-regulated miRNA during skin wound healing and its inhibition impairs wound repair. In a human in vivo surgical wound model, we showed that miR-132 was induced in epidermal as well as in dermal wound–edge compartments during healing. Moreover, in a panel of cells isolated from human skin wounds, miR-132 was found highly expressed in human dermal fibroblasts (HDFs). In HDFs, miR-132 expression was upregulated by TGF-β1. By overexpression or inhibition of miR-132, we showed that miR-132 promoted HDF migration. Mechanistically, global transcriptome analysis revealed that RAS signaling pathway was regulated by miR-132 in HDFs. We found that RAS p21 protein activator 1 (RASA1), a known target of miR-132, was downregulated in HDFs upon miR-132 overexpression. Silencing of RASA1 phenocopied the pro-migratory effect of miR-132. Collectively, our study reveals an important role for miR-132 in HDFs during wound healing and indicates a therapeutic potential of miR-132 in hard-to-heal skin wounds.Xi LiDongqing LiJakob D. WikstromAndor PivarcsiEnikö SonkolyMona StåhleNing Xu LandénNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Xi Li
Dongqing Li
Jakob D. Wikstrom
Andor Pivarcsi
Enikö Sonkoly
Mona Ståhle
Ning Xu Landén
MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
description Abstract MicroRNA (miR)-132 has been identified as a top up-regulated miRNA during skin wound healing and its inhibition impairs wound repair. In a human in vivo surgical wound model, we showed that miR-132 was induced in epidermal as well as in dermal wound–edge compartments during healing. Moreover, in a panel of cells isolated from human skin wounds, miR-132 was found highly expressed in human dermal fibroblasts (HDFs). In HDFs, miR-132 expression was upregulated by TGF-β1. By overexpression or inhibition of miR-132, we showed that miR-132 promoted HDF migration. Mechanistically, global transcriptome analysis revealed that RAS signaling pathway was regulated by miR-132 in HDFs. We found that RAS p21 protein activator 1 (RASA1), a known target of miR-132, was downregulated in HDFs upon miR-132 overexpression. Silencing of RASA1 phenocopied the pro-migratory effect of miR-132. Collectively, our study reveals an important role for miR-132 in HDFs during wound healing and indicates a therapeutic potential of miR-132 in hard-to-heal skin wounds.
format article
author Xi Li
Dongqing Li
Jakob D. Wikstrom
Andor Pivarcsi
Enikö Sonkoly
Mona Ståhle
Ning Xu Landén
author_facet Xi Li
Dongqing Li
Jakob D. Wikstrom
Andor Pivarcsi
Enikö Sonkoly
Mona Ståhle
Ning Xu Landén
author_sort Xi Li
title MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
title_short MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
title_full MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
title_fullStr MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
title_full_unstemmed MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
title_sort microrna-132 promotes fibroblast migration via regulating ras p21 protein activator 1 in skin wound healing
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/edc64160f4cc426d8b6f4e11e8a1ef43
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