MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing

Abstract MicroRNA (miR)-132 has been identified as a top up-regulated miRNA during skin wound healing and its inhibition impairs wound repair. In a human in vivo surgical wound model, we showed that miR-132 was induced in epidermal as well as in dermal wound–edge compartments during healing. Moreove...

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Autores principales:	Xi Li, Dongqing Li, Jakob D. Wikstrom, Andor Pivarcsi, Enikö Sonkoly, Mona Ståhle, Ning Xu Landén
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/edc64160f4cc426d8b6f4e11e8a1ef43
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spelling	oai:doaj.org-article:edc64160f4cc426d8b6f4e11e8a1ef432021-12-02T15:06:03ZMicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing10.1038/s41598-017-07513-02045-2322https://doaj.org/article/edc64160f4cc426d8b6f4e11e8a1ef432017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07513-0https://doaj.org/toc/2045-2322Abstract MicroRNA (miR)-132 has been identified as a top up-regulated miRNA during skin wound healing and its inhibition impairs wound repair. In a human in vivo surgical wound model, we showed that miR-132 was induced in epidermal as well as in dermal wound–edge compartments during healing. Moreover, in a panel of cells isolated from human skin wounds, miR-132 was found highly expressed in human dermal fibroblasts (HDFs). In HDFs, miR-132 expression was upregulated by TGF-β1. By overexpression or inhibition of miR-132, we showed that miR-132 promoted HDF migration. Mechanistically, global transcriptome analysis revealed that RAS signaling pathway was regulated by miR-132 in HDFs. We found that RAS p21 protein activator 1 (RASA1), a known target of miR-132, was downregulated in HDFs upon miR-132 overexpression. Silencing of RASA1 phenocopied the pro-migratory effect of miR-132. Collectively, our study reveals an important role for miR-132 in HDFs during wound healing and indicates a therapeutic potential of miR-132 in hard-to-heal skin wounds.Xi LiDongqing LiJakob D. WikstromAndor PivarcsiEnikö SonkolyMona StåhleNing Xu LandénNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xi Li Dongqing Li Jakob D. Wikstrom Andor Pivarcsi Enikö Sonkoly Mona Ståhle Ning Xu Landén MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
description	Abstract MicroRNA (miR)-132 has been identified as a top up-regulated miRNA during skin wound healing and its inhibition impairs wound repair. In a human in vivo surgical wound model, we showed that miR-132 was induced in epidermal as well as in dermal wound–edge compartments during healing. Moreover, in a panel of cells isolated from human skin wounds, miR-132 was found highly expressed in human dermal fibroblasts (HDFs). In HDFs, miR-132 expression was upregulated by TGF-β1. By overexpression or inhibition of miR-132, we showed that miR-132 promoted HDF migration. Mechanistically, global transcriptome analysis revealed that RAS signaling pathway was regulated by miR-132 in HDFs. We found that RAS p21 protein activator 1 (RASA1), a known target of miR-132, was downregulated in HDFs upon miR-132 overexpression. Silencing of RASA1 phenocopied the pro-migratory effect of miR-132. Collectively, our study reveals an important role for miR-132 in HDFs during wound healing and indicates a therapeutic potential of miR-132 in hard-to-heal skin wounds.
format	article
author	Xi Li Dongqing Li Jakob D. Wikstrom Andor Pivarcsi Enikö Sonkoly Mona Ståhle Ning Xu Landén
author_facet	Xi Li Dongqing Li Jakob D. Wikstrom Andor Pivarcsi Enikö Sonkoly Mona Ståhle Ning Xu Landén
author_sort	Xi Li
title	MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
title_short	MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
title_full	MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
title_fullStr	MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
title_full_unstemmed	MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
title_sort	microrna-132 promotes fibroblast migration via regulating ras p21 protein activator 1 in skin wound healing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/edc64160f4cc426d8b6f4e11e8a1ef43
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MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing

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