MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing
Abstract MicroRNA (miR)-132 has been identified as a top up-regulated miRNA during skin wound healing and its inhibition impairs wound repair. In a human in vivo surgical wound model, we showed that miR-132 was induced in epidermal as well as in dermal wound–edge compartments during healing. Moreove...
Guardado en:
Autores principales: | , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2017
|
Materias: | |
Acceso en línea: | https://doaj.org/article/edc64160f4cc426d8b6f4e11e8a1ef43 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:edc64160f4cc426d8b6f4e11e8a1ef43 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:edc64160f4cc426d8b6f4e11e8a1ef432021-12-02T15:06:03ZMicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing10.1038/s41598-017-07513-02045-2322https://doaj.org/article/edc64160f4cc426d8b6f4e11e8a1ef432017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07513-0https://doaj.org/toc/2045-2322Abstract MicroRNA (miR)-132 has been identified as a top up-regulated miRNA during skin wound healing and its inhibition impairs wound repair. In a human in vivo surgical wound model, we showed that miR-132 was induced in epidermal as well as in dermal wound–edge compartments during healing. Moreover, in a panel of cells isolated from human skin wounds, miR-132 was found highly expressed in human dermal fibroblasts (HDFs). In HDFs, miR-132 expression was upregulated by TGF-β1. By overexpression or inhibition of miR-132, we showed that miR-132 promoted HDF migration. Mechanistically, global transcriptome analysis revealed that RAS signaling pathway was regulated by miR-132 in HDFs. We found that RAS p21 protein activator 1 (RASA1), a known target of miR-132, was downregulated in HDFs upon miR-132 overexpression. Silencing of RASA1 phenocopied the pro-migratory effect of miR-132. Collectively, our study reveals an important role for miR-132 in HDFs during wound healing and indicates a therapeutic potential of miR-132 in hard-to-heal skin wounds.Xi LiDongqing LiJakob D. WikstromAndor PivarcsiEnikö SonkolyMona StåhleNing Xu LandénNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Xi Li Dongqing Li Jakob D. Wikstrom Andor Pivarcsi Enikö Sonkoly Mona Ståhle Ning Xu Landén MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing |
description |
Abstract MicroRNA (miR)-132 has been identified as a top up-regulated miRNA during skin wound healing and its inhibition impairs wound repair. In a human in vivo surgical wound model, we showed that miR-132 was induced in epidermal as well as in dermal wound–edge compartments during healing. Moreover, in a panel of cells isolated from human skin wounds, miR-132 was found highly expressed in human dermal fibroblasts (HDFs). In HDFs, miR-132 expression was upregulated by TGF-β1. By overexpression or inhibition of miR-132, we showed that miR-132 promoted HDF migration. Mechanistically, global transcriptome analysis revealed that RAS signaling pathway was regulated by miR-132 in HDFs. We found that RAS p21 protein activator 1 (RASA1), a known target of miR-132, was downregulated in HDFs upon miR-132 overexpression. Silencing of RASA1 phenocopied the pro-migratory effect of miR-132. Collectively, our study reveals an important role for miR-132 in HDFs during wound healing and indicates a therapeutic potential of miR-132 in hard-to-heal skin wounds. |
format |
article |
author |
Xi Li Dongqing Li Jakob D. Wikstrom Andor Pivarcsi Enikö Sonkoly Mona Ståhle Ning Xu Landén |
author_facet |
Xi Li Dongqing Li Jakob D. Wikstrom Andor Pivarcsi Enikö Sonkoly Mona Ståhle Ning Xu Landén |
author_sort |
Xi Li |
title |
MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing |
title_short |
MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing |
title_full |
MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing |
title_fullStr |
MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing |
title_full_unstemmed |
MicroRNA-132 promotes fibroblast migration via regulating RAS p21 protein activator 1 in skin wound healing |
title_sort |
microrna-132 promotes fibroblast migration via regulating ras p21 protein activator 1 in skin wound healing |
publisher |
Nature Portfolio |
publishDate |
2017 |
url |
https://doaj.org/article/edc64160f4cc426d8b6f4e11e8a1ef43 |
work_keys_str_mv |
AT xili microrna132promotesfibroblastmigrationviaregulatingrasp21proteinactivator1inskinwoundhealing AT dongqingli microrna132promotesfibroblastmigrationviaregulatingrasp21proteinactivator1inskinwoundhealing AT jakobdwikstrom microrna132promotesfibroblastmigrationviaregulatingrasp21proteinactivator1inskinwoundhealing AT andorpivarcsi microrna132promotesfibroblastmigrationviaregulatingrasp21proteinactivator1inskinwoundhealing AT enikosonkoly microrna132promotesfibroblastmigrationviaregulatingrasp21proteinactivator1inskinwoundhealing AT monastahle microrna132promotesfibroblastmigrationviaregulatingrasp21proteinactivator1inskinwoundhealing AT ningxulanden microrna132promotesfibroblastmigrationviaregulatingrasp21proteinactivator1inskinwoundhealing |
_version_ |
1718388617329508352 |