An all-in-one UniSam vector system for efficient gene activation

Abstract We have generated a drug-free, all-in-one dCAS9-SAM vector that can activate endogenous gene expression with the potential to modify cell fate. We demonstrate that this strategy can be used in a number of cell lines and avoids exceptionally high levels of gene expression that are observed i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Antonella Fidanza, Martha Lopez-Yrigoyen, Nicola Romanò, Rhiannon Jones, A. Helen Taylor, Lesley M. Forrester
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/edc72eebc6cf414da606a273513ae452
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:edc72eebc6cf414da606a273513ae452
record_format	dspace
spelling	oai:doaj.org-article:edc72eebc6cf414da606a273513ae4522021-12-02T16:06:51ZAn all-in-one UniSam vector system for efficient gene activation10.1038/s41598-017-06468-62045-2322https://doaj.org/article/edc72eebc6cf414da606a273513ae4522017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06468-6https://doaj.org/toc/2045-2322Abstract We have generated a drug-free, all-in-one dCAS9-SAM vector that can activate endogenous gene expression with the potential to modify cell fate. We demonstrate that this strategy can be used in a number of cell lines and avoids exceptionally high levels of gene expression that are observed in standard transgenic approaches. Compared to the multi-plasmid system, this all-in-one vector activates gene expression to a comparable level but the reduced overall DNA content results in significantly higher viability of transfected cells. This allowed us to use the RUNX1C-GFP human embryonic stem cell reporter cell line to monitor gene activation in individual cells and to show that activation could occur at all stages of the cell cycle.Antonella FidanzaMartha Lopez-YrigoyenNicola RomanòRhiannon JonesA. Helen TaylorLesley M. ForresterNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Antonella Fidanza Martha Lopez-Yrigoyen Nicola Romanò Rhiannon Jones A. Helen Taylor Lesley M. Forrester An all-in-one UniSam vector system for efficient gene activation
description	Abstract We have generated a drug-free, all-in-one dCAS9-SAM vector that can activate endogenous gene expression with the potential to modify cell fate. We demonstrate that this strategy can be used in a number of cell lines and avoids exceptionally high levels of gene expression that are observed in standard transgenic approaches. Compared to the multi-plasmid system, this all-in-one vector activates gene expression to a comparable level but the reduced overall DNA content results in significantly higher viability of transfected cells. This allowed us to use the RUNX1C-GFP human embryonic stem cell reporter cell line to monitor gene activation in individual cells and to show that activation could occur at all stages of the cell cycle.
format	article
author	Antonella Fidanza Martha Lopez-Yrigoyen Nicola Romanò Rhiannon Jones A. Helen Taylor Lesley M. Forrester
author_facet	Antonella Fidanza Martha Lopez-Yrigoyen Nicola Romanò Rhiannon Jones A. Helen Taylor Lesley M. Forrester
author_sort	Antonella Fidanza
title	An all-in-one UniSam vector system for efficient gene activation
title_short	An all-in-one UniSam vector system for efficient gene activation
title_full	An all-in-one UniSam vector system for efficient gene activation
title_fullStr	An all-in-one UniSam vector system for efficient gene activation
title_full_unstemmed	An all-in-one UniSam vector system for efficient gene activation
title_sort	all-in-one unisam vector system for efficient gene activation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/edc72eebc6cf414da606a273513ae452
work_keys_str_mv	AT antonellafidanza anallinoneunisamvectorsystemforefficientgeneactivation AT marthalopezyrigoyen anallinoneunisamvectorsystemforefficientgeneactivation AT nicolaromano anallinoneunisamvectorsystemforefficientgeneactivation AT rhiannonjones anallinoneunisamvectorsystemforefficientgeneactivation AT ahelentaylor anallinoneunisamvectorsystemforefficientgeneactivation AT lesleymforrester anallinoneunisamvectorsystemforefficientgeneactivation AT antonellafidanza allinoneunisamvectorsystemforefficientgeneactivation AT marthalopezyrigoyen allinoneunisamvectorsystemforefficientgeneactivation AT nicolaromano allinoneunisamvectorsystemforefficientgeneactivation AT rhiannonjones allinoneunisamvectorsystemforefficientgeneactivation AT ahelentaylor allinoneunisamvectorsystemforefficientgeneactivation AT lesleymforrester allinoneunisamvectorsystemforefficientgeneactivation
_version_	1718384877025361920

An all-in-one UniSam vector system for efficient gene activation

Ejemplares similares