An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches

Abstract Strict anaerobes are undeniably important residents of the cystic fibrosis (CF) lung but are still unknowns. The main objectives of this study were to describe anaerobic bacteria diversity in CF airway microbiota and to evaluate the association with lung function. An observational study was...

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Autores principales:	Claudie Lamoureux, Charles-Antoine Guilloux, Clémence Beauruelle, Stéphanie Gouriou, Sophie Ramel, Anne Dirou, Jean Le Bihan, Krista Revert, Thomas Ropars, Rosyne Lagrafeuille, Sophie Vallet, Rozenn Le Berre, Emmanuel Nowak, Geneviève Héry-Arnaud
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/edc865cbc470426ab7f11e527b09504e
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spelling	oai:doaj.org-article:edc865cbc470426ab7f11e527b09504e2021-12-02T11:45:04ZAn observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches10.1038/s41598-021-85592-w2045-2322https://doaj.org/article/edc865cbc470426ab7f11e527b09504e2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85592-whttps://doaj.org/toc/2045-2322Abstract Strict anaerobes are undeniably important residents of the cystic fibrosis (CF) lung but are still unknowns. The main objectives of this study were to describe anaerobic bacteria diversity in CF airway microbiota and to evaluate the association with lung function. An observational study was conducted during eight months. A hundred and one patients were enrolled in the study, and 150 sputum samples were collected using a sterile sample kit designed to preserve anaerobic conditions. An extended-culture approach on 112 sputa and a molecular approach (quantitative PCR targeting three of the main anaerobic genera in CF lung: Prevotella, Veillonella, and Fusobacterium) on 141 sputa were developed. On culture, 91.1% of sputa were positive for at least one anaerobic bacterial species, with an average of six anaerobic species detected per sputum. Thirty-one anaerobic genera and 69 species were found, which is the largest anaerobe diversity ever reported in CF lungs. Better lung function (defined as Forced Expiratory Volume in one second > 70%) was significantly associated with higher quantification of Veillonella. These results raise the question of the potential impact of anaerobes on lung function.Claudie LamoureuxCharles-Antoine GuillouxClémence BeauruelleStéphanie GouriouSophie RamelAnne DirouJean Le BihanKrista RevertThomas RoparsRosyne LagrafeuilleSophie ValletRozenn Le BerreEmmanuel NowakGeneviève Héry-ArnaudNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claudie Lamoureux Charles-Antoine Guilloux Clémence Beauruelle Stéphanie Gouriou Sophie Ramel Anne Dirou Jean Le Bihan Krista Revert Thomas Ropars Rosyne Lagrafeuille Sophie Vallet Rozenn Le Berre Emmanuel Nowak Geneviève Héry-Arnaud An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches
description	Abstract Strict anaerobes are undeniably important residents of the cystic fibrosis (CF) lung but are still unknowns. The main objectives of this study were to describe anaerobic bacteria diversity in CF airway microbiota and to evaluate the association with lung function. An observational study was conducted during eight months. A hundred and one patients were enrolled in the study, and 150 sputum samples were collected using a sterile sample kit designed to preserve anaerobic conditions. An extended-culture approach on 112 sputa and a molecular approach (quantitative PCR targeting three of the main anaerobic genera in CF lung: Prevotella, Veillonella, and Fusobacterium) on 141 sputa were developed. On culture, 91.1% of sputa were positive for at least one anaerobic bacterial species, with an average of six anaerobic species detected per sputum. Thirty-one anaerobic genera and 69 species were found, which is the largest anaerobe diversity ever reported in CF lungs. Better lung function (defined as Forced Expiratory Volume in one second > 70%) was significantly associated with higher quantification of Veillonella. These results raise the question of the potential impact of anaerobes on lung function.
format	article
author	Claudie Lamoureux Charles-Antoine Guilloux Clémence Beauruelle Stéphanie Gouriou Sophie Ramel Anne Dirou Jean Le Bihan Krista Revert Thomas Ropars Rosyne Lagrafeuille Sophie Vallet Rozenn Le Berre Emmanuel Nowak Geneviève Héry-Arnaud
author_facet	Claudie Lamoureux Charles-Antoine Guilloux Clémence Beauruelle Stéphanie Gouriou Sophie Ramel Anne Dirou Jean Le Bihan Krista Revert Thomas Ropars Rosyne Lagrafeuille Sophie Vallet Rozenn Le Berre Emmanuel Nowak Geneviève Héry-Arnaud
author_sort	Claudie Lamoureux
title	An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches
title_short	An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches
title_full	An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches
title_fullStr	An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches
title_full_unstemmed	An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches
title_sort	observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/edc865cbc470426ab7f11e527b09504e
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An observational study of anaerobic bacteria in cystic fibrosis lung using culture dependant and independent approaches

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