Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits

Abstract Background Cartilage damage is a common medical issue in clinical practice. Complete cartilage repair remains a significant challenge owing to the inferior quality of regenerative tissue. Safe and non-invasive magnetic therapy combined with tissue engineering to repair cartilage may be a pr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiyuan Yan, Chaoxu Liu, Chang Tu, Ruizhuo Zhang, Xiangyu Tang, Hao Li, Huaixi Wang, Yongzhuang Ma, Yingchi Zhang, Hua Wu, Gaohong Sheng
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Hydrogel Hydroxyapatite Monomeric Collagen type I (Col1) Electromagnetic fields Osteochondral defects Mesenchymal stem cells Medicine (General) R5-920 Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/edd0c633f2bd45948604944c65a74d95
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:edd0c633f2bd45948604944c65a74d95
record_format	dspace
spelling	oai:doaj.org-article:edd0c633f2bd45948604944c65a74d952021-11-14T12:08:05ZHydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits10.1186/s13287-021-02638-61757-6512https://doaj.org/article/edd0c633f2bd45948604944c65a74d952021-11-01T00:00:00Zhttps://doi.org/10.1186/s13287-021-02638-6https://doaj.org/toc/1757-6512Abstract Background Cartilage damage is a common medical issue in clinical practice. Complete cartilage repair remains a significant challenge owing to the inferior quality of regenerative tissue. Safe and non-invasive magnetic therapy combined with tissue engineering to repair cartilage may be a promising breakthrough. Methods In this study, a composite scaffold made of Hydroxyapatite-Collagen type-I (HAC) and PLGA-PEG-PLGA thermogel was produced to match the cartilage and subchondral layers in osteochondral defects, respectively. Bone marrow mesenchymal stem cells (BMSC) encapsulated in the thermogel were stimulated by an electromagnetic field (EMF). Effect of EMF on the proliferation and chondrogenic differentiation potential was evaluated in vitro. 4 mm femoral condyle defect was constructed in rabbits. The scaffolds loaded with BMSCs were implanted into the defects with or without EMF treatment. Effects of the combination treatment of the EMF and composite scaffold on rabbit osteochondral defect was detected in vivo. Results In vitro experiments showed that EMF could promote proliferation and chondrogenic differentiation of BMSCs partly by activating the PI3K/AKT/mTOR and Wnt1/LRP6/β-catenin signaling pathway. In vivo results further confirmed that the scaffold with EMF enhances the repair of osteochondral defects in rabbits, and, in particular, cartilage repair. Conclusion Hydrogel-Hydroxyapatite-Monomeric Collagen type-I scaffold with low-frequency EMF treatment has the potential to enhance osteochondral repair.Jiyuan YanChaoxu LiuChang TuRuizhuo ZhangXiangyu TangHao LiHuaixi WangYongzhuang MaYingchi ZhangHua WuGaohong ShengBMCarticleHydrogelHydroxyapatiteMonomeric Collagen type I (Col1)Electromagnetic fieldsOsteochondral defectsMesenchymal stem cellsMedicine (General)R5-920BiochemistryQD415-436ENStem Cell Research & Therapy, Vol 12, Iss 1, Pp 1-21 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hydrogel Hydroxyapatite Monomeric Collagen type I (Col1) Electromagnetic fields Osteochondral defects Mesenchymal stem cells Medicine (General) R5-920 Biochemistry QD415-436
spellingShingle	Hydrogel Hydroxyapatite Monomeric Collagen type I (Col1) Electromagnetic fields Osteochondral defects Mesenchymal stem cells Medicine (General) R5-920 Biochemistry QD415-436 Jiyuan Yan Chaoxu Liu Chang Tu Ruizhuo Zhang Xiangyu Tang Hao Li Huaixi Wang Yongzhuang Ma Yingchi Zhang Hua Wu Gaohong Sheng Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits
description	Abstract Background Cartilage damage is a common medical issue in clinical practice. Complete cartilage repair remains a significant challenge owing to the inferior quality of regenerative tissue. Safe and non-invasive magnetic therapy combined with tissue engineering to repair cartilage may be a promising breakthrough. Methods In this study, a composite scaffold made of Hydroxyapatite-Collagen type-I (HAC) and PLGA-PEG-PLGA thermogel was produced to match the cartilage and subchondral layers in osteochondral defects, respectively. Bone marrow mesenchymal stem cells (BMSC) encapsulated in the thermogel were stimulated by an electromagnetic field (EMF). Effect of EMF on the proliferation and chondrogenic differentiation potential was evaluated in vitro. 4 mm femoral condyle defect was constructed in rabbits. The scaffolds loaded with BMSCs were implanted into the defects with or without EMF treatment. Effects of the combination treatment of the EMF and composite scaffold on rabbit osteochondral defect was detected in vivo. Results In vitro experiments showed that EMF could promote proliferation and chondrogenic differentiation of BMSCs partly by activating the PI3K/AKT/mTOR and Wnt1/LRP6/β-catenin signaling pathway. In vivo results further confirmed that the scaffold with EMF enhances the repair of osteochondral defects in rabbits, and, in particular, cartilage repair. Conclusion Hydrogel-Hydroxyapatite-Monomeric Collagen type-I scaffold with low-frequency EMF treatment has the potential to enhance osteochondral repair.
format	article
author	Jiyuan Yan Chaoxu Liu Chang Tu Ruizhuo Zhang Xiangyu Tang Hao Li Huaixi Wang Yongzhuang Ma Yingchi Zhang Hua Wu Gaohong Sheng
author_facet	Jiyuan Yan Chaoxu Liu Chang Tu Ruizhuo Zhang Xiangyu Tang Hao Li Huaixi Wang Yongzhuang Ma Yingchi Zhang Hua Wu Gaohong Sheng
author_sort	Jiyuan Yan
title	Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits
title_short	Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits
title_full	Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits
title_fullStr	Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits
title_full_unstemmed	Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits
title_sort	hydrogel-hydroxyapatite-monomeric collagen type-i scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits
publisher	BMC
publishDate	2021
url	https://doaj.org/article/edd0c633f2bd45948604944c65a74d95
work_keys_str_mv	AT jiyuanyan hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits AT chaoxuliu hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits AT changtu hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits AT ruizhuozhang hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits AT xiangyutang hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits AT haoli hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits AT huaixiwang hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits AT yongzhuangma hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits AT yingchizhang hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits AT huawu hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits AT gaohongsheng hydrogelhydroxyapatitemonomericcollagentypeiscaffoldwithlowfrequencyelectromagneticfieldtreatmentenhancesosteochondralrepairinrabbits
_version_	1718429440222953472

Hydrogel-hydroxyapatite-monomeric collagen type-I scaffold with low-frequency electromagnetic field treatment enhances osteochondral repair in rabbits

Ejemplares similares